High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Han, Sumin; Im, Kang Bin; Park, Hwajoon; Kim, Dong–Sun; Kim, Beop Min

doi:10.1117/12.591465

Cited by 3 publications

(2 citation statements)

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“…This approach has been in use along with acousto-optical devices, generating frame rates in excess of 100 Hz (Han, Im, Park, Kim, & Kim, 2005), or in combination with light sheet excitation to improve optical sectioning (Baumgart & Kubitscheck, 2012). The principle behind this approach is to increase the vertical size of galvanometer sweeps, and thus increase the rates of acquisition.…”

Section: Of 17mentioning

confidence: 99%

“…The principle behind this approach is to increase the vertical size of galvanometer sweeps, and thus increase the rates of acquisition. This approach has been in use along with acousto-optical devices, generating frame rates in excess of 100 Hz (Han, Im, Park, Kim, & Kim, 2005), or in combination with light sheet excitation to improve optical sectioning (Baumgart & Kubitscheck, 2012). Slit-confocal microscopy is limited by reduced resolution and optical sectioning abilities, when compared with point-scanning approaches.…”

Section: Bayguinov Et Almentioning

confidence: 99%

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Modern Laser Scanning Confocal Microscopy

et al. 2018

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Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three-dimensional structural studies of biological cells and tissues. The flexibility of the approach has enabled its application in a diverse array of studies, from the fast imaging of dynamic processes in living cells, to meticulous morphological analyses of tissues, and co-localization of protein expression patterns. In this chapter, we introduce the principles of confocal microscopy and discuss how the approach has become a mainstay in the biological sciences. We describe the components of a CLSM system and assess how modern implementations of the approach have further expanded the use of the technique. Finally, we briefly outline some practical considerations to take into account when acquiring data using a CLSM system. © 2018 by John Wiley & Sons, Inc.

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Section: Of 17mentioning

confidence: 99%

Section: Bayguinov Et Almentioning

confidence: 99%

Modern Laser Scanning Confocal Microscopy

et al. 2018

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三维表面形貌测量中的共聚焦显微成像技术研究进展

Wang Yueying,

Liu Xu,

Hao Xiang

2023

激光与光电子学进展

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光谱共焦显微技术研究进展

Ding Wanyun,

Wang Yuhang,

Zhang Tao

et al. 2024

激光与光电子学进展

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Chromatic confocal microscopy (CCM) combines the high spatial resolution of confocal microscope and the high wavelength resolution of spectral analysis. By virtue of the high precision, strong applicability, and nondestructive detection, it is widely used in the fields of industrial production, biomedicine, semiconductor chips, and other fields. This paper first introduces the principle of point chromatic confocal system and points out its drawback of low detection efficiency. Second, for improving the key performance indexes of chromatic confocal microscopy, the main achievements made in light source, dispersive objective lens, and spectral signal detection are described, and qualitative comparisons are made between various types of light sources. Subsequently, the scanning methods of chromatic confocal microscopy are demonstrated, the relevant research progress is sorted out, and the advantages and disadvantages of the relevant methods are summarized. Finally, the future developments of chromatic confocal microscopy are also prospected.

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High speed slit-scanning confocal laser microscopy with an acousto-optic beam deflector and a line scan camera

Cited by 3 publications

References 0 publications

Modern Laser Scanning Confocal Microscopy

Modern Laser Scanning Confocal Microscopy

三维表面形貌测量中的共聚焦显微成像技术研究进展

光谱共焦显微技术研究进展

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