High-speed video gait analysis reveals early and characteristic locomotor phenotypes in mouse models of neurodegenerative movement disorders

Preisig, Daniel F.; Kulic, Luka; Krüger, Maik; Wirth, Fabian; McAfoose, Jordan; Späni, Claudia; Gantenbein, Pascal; Derungs, Rebecca; Nitsch, Roger M.; Welt, Tobias

doi:10.1016/j.bbr.2016.04.044

Cited by 40 publications

(47 citation statements)

References 46 publications

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“…SOD1 G93A mice moved slowly with alternating lateral displacements of the body, which were represented by characteristic zigzag tracks at the 30 fps video sampling rate (Figure 3D, Supplementary Videos 3, 4). This tottering movement of SOD1 G93A mice may be related to the gait differences reported previously for this ALS animal model (18–21). Meanwhile, wild-type mice moved quickly and balanced, yielding smooth tracks without zigzag tracks.…”

Section: Resultssupporting

confidence: 67%

Mouse Behavior Tracker: An Economical Method for Tracking Behavior in Home Cages

et al. 2017

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Analysis of mouse behavior often requires expensive equipment and transfer of the mice to new test en- vironments, which could trigger confounding behavior alterations. Here, we describe a system for track- ing mouse behavior in home cages using a low-cost USB webcam and free software (Fiji and wrMTrck). We demonstrate the effectiveness of this method by tracking differences in distance traveled, speed, and movement tracks between wild-type mice and amyotrophic lateral sclerosis (ALS) model mice (SOD1G93A).

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Section: Resultssupporting

confidence: 67%

Mouse Behavior Tracker: An Economical Method for Tracking Behavior in Home Cages

et al. 2017

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“…The high-speed video analysis system MotoRater (TSE Systems, Bad Homburg, Germany) was used to evaluate the gait pattern of intracerebroventricularly treated SOD1 G93A tg animals according to Preisig et al (21). Motor coordination was measured using a rotarod apparatus (Ugo Basile, Monvalle, Italy).…”

Section: Motor Performance Assessment By Video Gait Analysis Rotarodmentioning

confidence: 99%

A human-derived antibody targets misfolded SOD1 and ameliorates motor symptoms in mouse models of amyotrophic lateral sclerosis

et al. 2018

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Mutations in the gene encoding superoxide dismutase 1 (SOD1) lead to misfolding and aggregation of SOD1 and cause familial amyotrophic lateral sclerosis (FALS). However, the implications of wild-type SOD1 misfolding in sporadic forms of ALS (SALS) remain unclear. By screening human memory B cells from a large cohort of healthy elderly subjects, we generated a recombinant human monoclonal antibody (α-miSOD1) that selectively bound to misfolded SOD1, but not to physiological SOD1 dimers. On postmortem spinal cord sections from 121 patients with ALS, α-miSOD1 antibody identified misfolded SOD1 in a majority of cases, regardless of their SOD1 genotype. In contrast, the α-miSOD1 antibody did not bind to its epitope in most of the 41 postmortem spinal cord sections from non-neurological control (NNC) patients. In transgenic mice overexpressing disease-causing human SOD1G37R or SOD1G93A mutations, treatment with the α-miSOD1 antibody delayed the onset of motor symptoms, extended survival by up to 2 months, and reduced aggregation of misfolded SOD1 and motor neuron degeneration. These effects were obtained whether α-miSOD1 antibody treatment was administered by direct brain infusion or peripheral administration. These results support the further development of α-miSOD1 antibody as a candidate treatment for ALS involving misfolding of SOD1.

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“…Many aspects of movement, such as locomotion and balance, are affected in neurological conditions (Mandillo et al, 2014;Preisig et al, 2016;Tucci et al, 2007). This has driven the development of a large number of tests, each recording different neuromuscular parameters, such as activity, motor coordination, and grip strength (Carter et al, 1999;Fernagut, Diguet, Labattu, & Tison, 2002;Tucci et al, 2007;Wooley et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…This has driven the development of a large number of tests, each recording different neuromuscular parameters, such as activity, motor coordination, and grip strength (Carter et al, 1999;Fernagut, Diguet, Labattu, & Tison, 2002;Tucci et al, 2007;Wooley et al, 2005). However, these tests can be influenced by external factors such as experimenter expertise, testing conditions, and the motivation of the test subject (Preisig et al, 2016), which will inevitably lead to experimental variability. Consequently, the reproducibility of test outcomes across laboratories can be an issue.…”

Section: Introductionmentioning

confidence: 99%

Phenotyping in Mice Using Continuous Home Cage Monitoring and Ultrasonic Vocalization Recordings

et al. 2020

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Over the last century, the study of mouse behavior has uncovered insights into brain molecular mechanisms while revealing potential causes of many neurological disorders. To this end, researchers have widely exploited the use of mutant strains, including those generated in mutagenesis screens and those produced using increasingly sophisticated genome engineering technologies. It is now relatively easy to access mouse models carrying alleles that faithfully recapitulate changes found in human patients or bearing variants of genes that provide data on those genes’ functions. Concurrent with these developments has been an appreciation of the limitations of some current testing platforms, especially those monitoring complex behaviors. Out‐of‐cage observational testing is useful in describing overt persistent phenotypes but risks missing sporadic or intermittent events. Furthermore, measuring the progression of a phenotype, potentially over many months, can be difficult while relying on assays that may be susceptible to changes in the testing environment. In recent years, there has also been increasing awareness that measurement of behaviors in isolation can be limiting, given that mice attempt to hide behavioral cues of vulnerability. To overcome these limitations, laboratory animal science is capitalizing on progress in data capture and processing expertise. Moreover, as additional recording modes become commonplace, ultrasonic vocalization recording is an appealing focus, as mice use vocalizations in various social contexts. Using video and audio technologies, we record the voluntary, unprovoked behaviors and vocalizations of mice in social groups. Adoption of these approaches is undoubtedly set to increase, as they capture the round‐the‐clock behavior of mouse strains. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Continuous recording of home cage activity using the Home Cage Analyzer (HCA) system Support Protocol: Subcutaneous insertion of a radio frequency identification microchip in the inguinal area Basic Protocol 2: Continuous recording of mouse ultrasonic vocalizations in the home cage.

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High-speed video gait analysis reveals early and characteristic locomotor phenotypes in mouse models of neurodegenerative movement disorders

Cited by 40 publications

References 46 publications

Mouse Behavior Tracker: An Economical Method for Tracking Behavior in Home Cages

Mouse Behavior Tracker: An Economical Method for Tracking Behavior in Home Cages

A human-derived antibody targets misfolded SOD1 and ameliorates motor symptoms in mouse models of amyotrophic lateral sclerosis

Phenotyping in Mice Using Continuous Home Cage Monitoring and Ultrasonic Vocalization Recordings

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