High starch duckweed biomass production and its highly-efficient conversion to bioethanol

Guo, Ling; Fang, Yang; Jin, Yanling; He, Kai; Zhao, Hai

doi:10.1016/j.eti.2023.103296

Cited by 5 publications

(1 citation statement)

References 42 publications

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“…For these reasons, we aimed to screen endogenous strong promoters of duckweed for application in transgenic technology to augment the expression of exogenous proteins and explore potential molecular mechanisms. Duckweed exhibits a simple leaf–stem structure known as a frond, yet it demonstrates rapid growth, high starch accumulation capacity, and exceptional photosynthetic efficiency ( Chen et al., 2022 ; Guo et al., 2023 ). In various plant species, promoters such as the sucrose transporter 2 promoter, Rubisco promoter, ubiquitin promoter, and others have demonstrated favorable outcomes in transgenic technology ( Christensen and Quail, 1996 ; Stadler and Sauer, 2019 ; Boruah et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Wei,

Hu,

Wang

et al. 2024

Front. Plant Sci.

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Promoters are one of the most critical elements in regulating gene expression. They are considered essential biotechnological tools for heterologous protein production. The one most widely used in plants is the 35S promoter from cauliflower mosaic virus. However, our study for the first time discovered the 35S promoter reduced the expression of exogenous proteins under increased antibiotic stress. We discovered an endogenous strong promoter from duckweed named LpSUT2 that keeps higher initiation activity under antibiotic stress. Stable transformation in duckweed showed that the gene expression of eGFP in the LpSUT2:eGFP was 1.76 times that of the 35S:eGFP at 100 mg.L-1 G418 and 6.18 times at 500 mg.L-1 G418. Notably, with the increase of G418 concentration, the gene expression and the fluorescence signal of eGFP in the 35S:eGFP were weakened, while the LpSUT2:eGFP only changed slightly. This is because, under high antibiotic stress, the 35S promoter was methylated, leading to the gene silencing of the eGFP gene. Meanwhile, the LpSUT2 promoter was not methylated and maintained high activity. This is a previously unknown mechanism that provides us with new insights into screening more stable promoters that are less affected by environmental stress. These outcomes suggest that the LpSUT2 promoter has a high capacity to initiate the expression of exogenous proteins. In conclusion, our study provides a promoter tool with potential application for plant genetic engineering and also provides new insights into screening promoters.

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Section: Introductionmentioning

confidence: 99%

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Wei,

Hu,

Wang

et al. 2024

Front. Plant Sci.

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Screening potential antileukemia agents from duckweed: Integration of chemical profiling, network pharmacology, and experimental validation

Liu,

Huang,

Yang

et al. 2024

Phytochemical Analysis

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IntroductionThe identification of active dietary flavonoids in food is promising for novel drug discovery. The active ingredients of duckweed (a widely recognized food and herb with abundant flavonoids) that are associated with acute myeloid leukemia (AML) have yet to be identified, and their underlying mechanisms have not been elucidated.ObjectivesThe objective of this study was to identify novel constituents exhibiting antileukemia activity in duckweed through the integration of chemical profiling, network pharmacology, and experimental validation.MethodsFirst, high performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS) was used to characterize the primary constituents of duckweed. Subsequently, AML cell‐xenograft tumor models were used to validate the anticancer effect of duckweed extract. Furthermore, network pharmacology analysis was conducted to predict the potential active compounds and drug targets against AML. Lastly, based on these findings, two monomers (apiin and luteoloside) were selected for experimental validation.ResultsA total of 17 compounds, all of which are apigenin and luteolin derivatives, were identified in duckweed. The duckweed extract significantly inhibited AML cell growth in vivo. Furthermore, a total of 88 targets for duckweed against AML were predicted, with key targets including PTGS2, MYC, MDM2, VEGFA, CTNNB1, CASP3, EGFR, TP53, HSP90AA1, CCND1, MMP9, TNF, and MAPK1. GO and KEGG pathway enrichment analyses indicated that these targets were primarily involved in the apoptotic signaling pathway. Lastly, both apiin and luteoloside effectively induced apoptosis through CASP3 activation, and this effect could be partially reversed by a caspase inhibitor (Z‐VAD).ConclusionDuckweed extract has an antileukemic effect, and apiin derived from duckweed shows potential as a treatment for AML.

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Phytoremediation potential of duckweed (Lemna minor L.) for hexavalent chromium removal in synthetic wastewater: unveiling physiological response and defense mechanisms against excessive heavy metal uptake

Aslanzadeh,

Saboora,

Moradlou

2024

Int. J. Environ. Sci. Technol.

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High starch duckweed biomass production and its highly-efficient conversion to bioethanol

Cited by 5 publications

References 42 publications

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants

Screening potential antileukemia agents from duckweed: Integration of chemical profiling, network pharmacology, and experimental validation

Phytoremediation potential of duckweed (Lemna minor L.) for hexavalent chromium removal in synthetic wastewater: unveiling physiological response and defense mechanisms against excessive heavy metal uptake

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