High-sugar, high-fat, and high-protein diets promote antibiotic resistance gene spreading in the mouse intestinal microbiota

Tan, Rong; Jin, Min; Shao, Yi-fan; Yin, Jing; Li, Haibei; Chen, Tianjiao; Shi, Danyang; Zhou, Shuqing; Lǐ, Jùnwén; Yang, Dong

doi:10.1080/19490976.2021.2022442

Cited by 32 publications

(21 citation statements)

References 67 publications

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“…Moreover, the significant shifts in the gut microbial composition after mcr-1 addition, and the increase in the abundance of genes enriched in plasmid transfer and diffusion pathways, formed an intestinal microenvironment that might promote the spread of ARGs. 36 These ARGs biomarkers were distributed in different bacteria, especially probiotics, opportunistic pathogens, and intestinal symbionts, which might interact with the host and result in damaged health.…”

Section: Discussionmentioning

confidence: 99%

Exogenous antibiotic resistance gene contributes to intestinal inflammation by modulating the gut microbiome and inflammatory cytokine responses in mouse

Tan¹,

Jin²,

Chen³

et al. 2022

Gut Microbes

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Dysregulation of the gut microbiota by environmental factors is associated with a variety of autoimmune and immune-mediated diseases. In addition, naturally-occurring extracellular antibiotic resistance genes (eARGs) might directly enter the gut via the food chain. However, following gut microbiota exposure to eARGs, the ecological processes shaping the microbiota community assembly, as well as the interplay between the microbiota composition, metabolic function, and the immune responses, are not well understood. Increasing focus on the One Health approach has led to an urgent need to investigate the direct health damage caused by eARGs. Herein, we reveal the significant influence of eARGs on microbiota communities, strongly driven by stochastic processes. How eARGs-stimulate variations in the composition and metabolomic function of the gut microbiota led to cytokine responses in mice of different age and sex were investigated. The results revealed that cytokines were significantly associated with immunomodulatory microbes, metabolites, and ARGs biomarkers. Cytokine production was associated with specific metabolic pathways (arachidonic acid and tryptophan metabolic pathways), as confirmed by ex vivo cytokine responses and recovery experiments in vivo . Furthermore, the gut microbial profile could be applied to accurately predict the degree of intestinal inflammation ascribed to the eARGs (area under the curve = 0.9616). The present study provided a comprehensive understanding of the influence of an eARGs on immune responses and intestinal barrier damage, shedding light on the interplay between eARGs, microbial, metabolites, and the gut antibiotic resistome in modulating the human immune system.

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Section: Discussionmentioning

confidence: 99%

Exogenous antibiotic resistance gene contributes to intestinal inflammation by modulating the gut microbiome and inflammatory cytokine responses in mouse

Tan¹,

Jin²,

Chen³

et al. 2022

Gut Microbes

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“…Inflammatory host responses triggered by the gut immune system (in inflammatory bowel disease patients) or by pathogens can suppress the anaerobic microbiota and boost enterobacterial colonization densities [ 63 , 64 ]. The increasing prevalence of carbapenemases and extended-spectrum beta-lactamase in the opportunistic pathogenic bacteria E. coli and K. pneumoniae is readily transmitted in Proteobacteria in the gut [ 65 ]. In a streptomycin-treated mouse model of Salmonella infection, mouse intestinal inflammation promotes the coproliferation of donor and recipient bacteria in the gut [ 66 , 67 ].…”

Section: In Vivo Horizontal Gene Transfer Of Bacterial Resistancementioning

confidence: 99%

“…e increasing prevalence of carbapenemases and extended-spectrum beta-lactamase in the opportunistic pathogenic bacteria E. coli and K. pneumoniae is readily transmitted in Proteobacteria in the gut [65]. In a streptomycin-treated mouse model of Salmonella infection, mouse intestinal inflammation promotes the coproliferation of donor and recipient bacteria in the gut [66,67].…”

Section: Horizontal Transfer Of Antibiotic Resistance Genes In Thementioning

confidence: 99%

The Spread of Antibiotic Resistance Genes In Vivo Model

Tao

Liang

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Infections caused by antibiotic-resistant bacteria are a major public health threat. The emergence and spread of antibiotic resistance genes (ARGs) in the environment or clinical setting pose a serious threat to human and animal health worldwide. Horizontal gene transfer (HGT) of ARGs is one of the main reasons for the dissemination of antibiotic resistance in vitro and in vivo environments. There is a consensus on the role of mobile genetic elements (MGEs) in the spread of bacterial resistance. Most drug resistance genes are located on plasmids, and the spread of drug resistance genes among microorganisms through plasmid-mediated conjugation transfer is the most common and effective way for the spread of multidrug resistance. Experimental studies of the processes driving the spread of antibiotic resistance have focused on simple in vitro model systems, but the current in vitro protocols might not correctly reflect the HGT of antibiotic resistance genes in realistic conditions. This calls for better models of how resistance genes transfer and disseminate in vivo. The in vivo model can better mimic the situation that occurs in patients, helping study the situation in more detail. This is crucial to develop innovative strategies to curtail the spread of antibiotic resistance genes in the future. This review aims to give an overview of the mechanisms of the spread of antibiotic resistance genes and then demonstrate the spread of antibiotic resistance genes in the in vivo model. Finally, we discuss the challenges in controlling the spread of antibiotic resistance genes and their potential solutions.

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“…Some studies have reported that through the intestinal microbiota, HFHS influences the gut-brain axis and plays an important role in affecting the secretion of gonadal hormone levels [ 19 ]. Tan et al found that in mice colonized with fecal microbiota from women with polycystic ovarian syndrome (PCOS), the ovarian function was altered following fecal microbiota transplantation [ 15 ]. Past studies showed that cisplatin-induced POF was associated with the intestinal microbiota [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…A few studies have demonstrated that intestinal microbiota plays a significant role in regulating host metabolism, immunity, intestinal barrier, and gut homeostasis [14]. Previous studies have proved that HFHS can alter the intestinal microbiota and affect the health of the host [15][16][17][18]. Some studies have reported that through the intestinal microbiota, HFHS influences the gut-brain axis and plays an important role in affecting the secretion of gonadal hormone levels [19].…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture May Inhibit Oxidative Stress of Premature Ovarian Failure Mice by Regulating Intestinal Microbiota

Geng

Nie

Ling

et al. 2022

Oxidative Medicine and Cellular Longevity

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Premature ovarian failure (POF) is the leading cause of female infertility, and there is no optimal treatment or medication available currently. For POF, electroacupuncture (EA) has been considered a promising therapeutic approach, but the mechanism for this is not clear. In this study, we explored the effects of EA (CV4, ST36, and SP6) on oxidative stress and intestinal microbiota of high-fat and high-sugar- (HFHS-) induced POF mice. The development of mice follicles was observed by hematoxylin and eosin (HE) staining. The serum levels of estrone (E1), estrogen (E2), estriol (E3), and 21-deoxycortisol (21D) were measured by the HPLC-MS/MS method. The concentrations of Fe2+, superoxide dismutase (SOD), hydroxyl radical (·OH), glutathione (GSH), superoxide anion, and malondialdehyde (MDA) were measured by spectrophotometry. The 16S-rDNA sequencing was used to measure many parameters related to the host gut bacteriome and mycobiome composition, relative abundance, and diversity. mRNA expression levels of ferroptosis-related genes were determined by RT-qPCR. After 4 weeks of EA intervention in POF mice, mature follicles were increased and the levels of the sex hormone were improved. SOD activities, antisuperoxide activities, and GSH increased while MDA, ·OH, and Fe2+ decreased. In addition, EA also altered the intestinal microbiota. These results reveal that EA can effectively inhibit ovarian oxidative stress and the accumulation of Fe2+ in POF mice. It may be that the alteration in the intestinal microbiota is one of the potential mechanisms of EA treatment. These findings suggest that EA has clinical potential as a safe treatment for POF.

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High-sugar, high-fat, and high-protein diets promote antibiotic resistance gene spreading in the mouse intestinal microbiota

Cited by 32 publications

References 67 publications

Exogenous antibiotic resistance gene contributes to intestinal inflammation by modulating the gut microbiome and inflammatory cytokine responses in mouse

Exogenous antibiotic resistance gene contributes to intestinal inflammation by modulating the gut microbiome and inflammatory cytokine responses in mouse

The Spread of Antibiotic Resistance Genes In Vivo Model

Electroacupuncture May Inhibit Oxidative Stress of Premature Ovarian Failure Mice by Regulating Intestinal Microbiota

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