High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium

Deng, Nan; Liu, Caixia; Chang, Ermei; Ji, Jing; Yao, Xiamei; Yue, Jianyun; Bartish, Igor V.; Chen, Lanzhen; Jiang, Zeping; Shi, Shengqing

doi:10.1016/j.ejbt.2016.11.001

Cited by 27 publications

(22 citation statements)

References 34 publications

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“…Genus Gnetum, comprising approximately 40 species, are woody climbers, with a few exceptions being shrubs and trees [1,10,11]. Most of Gnetum species, such as the Asian lianoid Gnetum, are rich in bioactive compounds, which can be used for medicine [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Long-Read Sequencing Reveals the Diversity of Full-Length Transcripts in Leaves of Gnetum (Gnetales)

Deng¹,

Hou

Ma³

et al. 2019

IJMS

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The limitations of RNA sequencing make it difficult to accurately predict alternative splicing (AS) and alternative polyadenylation (APA) events and long non-coding RNAs (lncRNAs), all of which reveal transcriptomic diversity and the complexity of gene regulation. Gnetum, a genus with ambiguous phylogenetic placement in seed plants, has a distinct stomatal structure and photosynthetic characteristics. In this study, a full-length transcriptome of Gnetum luofuense leaves at different developmental stages was sequenced with the latest PacBio Sequel platform. After correction by short reads generated by Illumina RNA-Seq, 80,496 full-length transcripts were obtained, of which 5269 reads were identified as isoforms of novel genes. Additionally, 1660 lncRNAs and 12,998 AS events were detected. In total, 5647 genes in the G. luofuense leaves had APA featured by at least one poly(A) site. Moreover, 67 and 30 genes from the bHLH gene family, which play an important role in stomatal development and photosynthesis, were identified from the G. luofuense genome and leaf transcripts, respectively. This leaf transcriptome supplements the reference genome of G. luofuense, and the AS events and lncRNAs detected provide valuable resources for future studies of investigating low photosynthetic capacity of Gnetum.

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Section: Introductionmentioning

confidence: 99%

Single-Molecule Long-Read Sequencing Reveals the Diversity of Full-Length Transcripts in Leaves of Gnetum (Gnetales)

Deng¹,

Hou

Ma³

et al. 2019

IJMS

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“…Recent results obtained by Deng et al (2016) showed that high temperature (40°C) induced expression of the PAL-, C4H-, 4CL-, and STS-like genes and increased piceatannol levels in the leaves of Gnetum parvifolium, but did not affect resveratrol levels. However, the following analysis revealed that the high temperature conditions inhibited the accumulation of both resveratrol and piceatannol in G. parvifolium stems and roots, while expression of the stilbene biosynthesis genes was increased with some variability (Deng et al 2017).…”

Section: Biotic and Abiotic Stresses As Regulators Of Stilbene Biosynmentioning

confidence: 99%

“…Multiple studies show that UV-C light exposure induced stilbene biosynthesis in different plant species (e.g., grapevine, pine, peanut, and gnetum), and the induction was accompanied by a transcriptional activation, protein accumulation, and enzyme activation of STS and some other stilbene biosynthesis enzymes (Wang et al 2010Tang et al 2010;Suzuki et al 2015;Deng et al 2016Deng et al , 2017Kiselev et al 2017). For grapevine berries, it has been shown that the induction of resveratrol biosynthesis after UV-C irradiation occurred primarily in the form of t-resveratrol (not piceids) and was dependent on the berry developmental stage .…”

Section: Biotic and Abiotic Stresses As Regulators Of Stilbene Biosynmentioning

confidence: 99%

Regulation of stilbene biosynthesis in plants

Dubrovina

Kiselev

2017

Planta

140

126

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Main conclusion This review analyzes the advances in understanding the natural signaling pathways and environmental factors regulating stilbene biosynthesis. We also discuss the studies reporting on stilbene content and repertoire in plants.Stilbenes, including the most-studied stilbene resveratrol, are a family of phenolic plant secondary metabolites that have been the subject of intensive research due to their valuable pharmaceutical effects and contribution to plant disease resistance. Understanding the natural mechanisms regulating stilbene biosynthesis in plants could be useful for both the development of new plant protection strategies and for commercial stilbene production. In this review, we focus on the environmental factors and cell signaling pathways regulating stilbene biosynthesis in plants and make a comparison with the regulation of flavonoid biosynthesis. This review also analyzes the recent data on stilbene biosynthetic genes and summarizes the available studies reporting on both stilbene content and stilbene composition in different plant families.

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“…Moreover, resveratrol derivative components such as gnemonoside C and gnetin C were extracted from G. gnemon seeds, and these compounds were shown to possess antiangiogenic effects [54]. This is probably the reason why Gnetum has antioxidant, anticancer, and antibacterial medicinal properties [10,11].…”

Section: Metabolism and Secondary Metabolitesmentioning

confidence: 99%

A Full-length Transcriptome and Gene Expression Analysis Reveal Genes and Molecular Elements Expressed During Seed Development in Gnetum Luofuense

Deng

Hou²,

et al. 2020

Preprint

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Abstract Background: Gnetum is an economically important tropical and subtropical gymnosperm genus with various dietary, industrial and medicinal uses. Many carbohydrates, proteins and secondary metabolic compounds accumulate during the ripening of Gnetum seeds.However, the molecular mechanisms related to this process remain unknown. Results: We therefore assembled a full-length transcriptome from immature and mature G. luofuenseseeds using PacBio sequencingreads. We identified a total of 5,726 novel genes, 9,061 alternative splicing events, 3,551 lncRNAs, 2,160 transcription factors, and 359 fusion genes, and we found that 8,512 genes possessed at least one poly(A) site. In addition, gene expression comparisons of six transcriptomes generated by Illumina sequencing showed that14,323 genes were differentially expressed from an immature stage to a mature stage with 7,891 genes upregulated and 6,432 genes downregulated. The expression of 14 differentially expressed transcription factors from the MADS-box, Aux/IAA and bHLH families was validated by qRT-PCR, suggesting that they may have important roles in seed ripening of G. luofuense. Conclusions:These findings provide a valuable molecular resource for domestication and cultivation of Gnetum species.

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High temperature and UV-C treatments affect stilbenoid accumulation and related gene expression levels in Gnetum parvifolium

Cited by 27 publications

References 34 publications

Single-Molecule Long-Read Sequencing Reveals the Diversity of Full-Length Transcripts in Leaves of Gnetum (Gnetales)

Single-Molecule Long-Read Sequencing Reveals the Diversity of Full-Length Transcripts in Leaves of Gnetum (Gnetales)

Regulation of stilbene biosynthesis in plants

A Full-length Transcriptome and Gene Expression Analysis Reveal Genes and Molecular Elements Expressed During Seed Development in Gnetum Luofuense

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