High-temperature EBPR process: The performance, analysis of PAOs and GAOs and the fine-scale population study of Candidatus “Accumulibacter phosphatis”

Ong, Ying Hui; Chua, Adeline Seak May; Fukushima, Toshikazu; Ngoh, Gek Cheng; Shoji, Tadashi; Michinaka, Atsuko

doi:10.1016/j.watres.2014.06.038

Cited by 99 publications

(51 citation statements)

References 34 publications

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“…Moreover, Yu et al (2014) revealed that higher COD loading would favor the growth of glycogen-accumulating organisms (GAOs) over PAOs, resulting the competing for carbon substrates in the anaerobic condition. The GAOs phenotype was clearly identified and the organisms were named ''Candidatus_Competibacter" in the previous studies (Oehmen et al, 2007;Ong et al, 2014). However, the proportion of Candidatus_Com-petibacter was below 3.5% in the three samples, which indicated that the GAOs was presented in relatively low fractions during the whole operation process in the system.…”

Section: High-through Pyrosequencing and Microbial Communitymentioning

confidence: 85%

Long term operation of continuous-flow system with enhanced biological phosphorus removal granules at different COD loading

Zeng

et al. 2016

Bioresource Technology

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Section: High-through Pyrosequencing and Microbial Communitymentioning

confidence: 85%

Long term operation of continuous-flow system with enhanced biological phosphorus removal granules at different COD loading

Zeng

et al. 2016

Bioresource Technology

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“…They indicated that an appropriate carbon concentration and dosing mode essentially determined DPR performance considering its provision of the main substrate source. A higher temperature was advantageous to accelerate the metabolism and growth speed of these clades (47). Anaerobic-released phosphorus positively relating with IID and IIF showed that the increases of these two clades were always followed by the increase of anaerobic P release.…”

Section: Resultsmentioning

confidence: 91%

Population Structure and Morphotype Analysis of “ Candidatus Accumulibacter” Using Fluorescence In Situ Hybridization-Staining-Flow Cytometry

Zeng

Guo

et al. 2019

Appl Environ Microbiol

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“Candidatus Accumulibacter” is the dominant polyphosphate-accumulating organism (PAO) in denitrifying phosphorus removal (DPR) systems. In order to investigate the community structure and clade morphotypes of “Candidatus Accumulibacter” in DPR systems through flow cytometry (FCM), denitrifying phosphorus removal of almost 100% using nitrite and nitrate as the electron acceptor was achieved in sequencing batch reactors (SBRs). An optimal method of flow cytometry combined with fluorescence in situ hybridization and SYBR green I staining (FISH-staining-flow cytometry) was developed to quantify PAOs in DPR systems. By setting the width value of FCM, bacterial cells in a sludge sample were divided into three groups in different morphotypes, namely, coccus, coccobacillus, and bacillus. Average percentages that the three different PAO populations accounted for among total bacteria from SBR1 (SBR2) were 42% (45%), 14% (13%), and 4% (2%). FCM showed that the ratios of PAOs to total bacteria in the two reactors were 61% and 59%, and the quantitative PCR (qPCR) results indicated that IIC was the dominant “Candidatus Accumulibacter” clade in both denitrifying phosphorus removal systems, reaching 50% of the total “Candidatus Accumulibacter” bacteria. The subdominant clade in the reactor with nitrite as the electron acceptor was IID, accounting for 31% of the total “Candidatus Accumulibacter” bacteria. The FCM and qPCR results suggested that clades IIC and IID were both coccus, clade IIF was coccobacillus, and clade IA was bacillus. FISH analysis also indicated that PAOs were major cocci in the systems. An equivalence test of FCM-based quantification confirmed the accuracy of FISH-staining-flow cytometry, which can meet the quantitative requirements for PAOs in complex activated sludge samples. IMPORTANCE As one group of the most important functional phosphorus removal organisms, “Candidatus Accumulibacter,” affiliated with the Rhodocyclus group of the Betaproteobacteria, is a widely recognized and studied PAO in the field of biological wastewater treatment. The morphotypes and population structure of clade-level “Candidatus Accumulibacter” were studied through novel FISH-staining-flow cytometry, which involved denitrifying phosphorus removal (DPR) achieving carbon and energy savings and simultaneous removal of N and P, thus inferring the different denitrifying phosphorus removal abilities of these clades. Additionally, based on this method, in situ quantification for specific polyphosphate-accumulating organisms (PAOs) enables a more efficient process and more accurate result. The establishment of FISH-staining-flow cytometry makes cell sorting of clade-level noncultivated organisms available.

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“…Generally, the aggregation of GAO is not conducive to phosphorus removal. However, it has also been reported that the abundance of GAO exceeds that of PAO at 32℃, but the removal effect is not affected [45]. Factors known that affect the abundance of PAO and GAO include carbon source, temperature and pH value [46][47][48].…”

Section: Variation Of the Diversity Of Microbial Communities On Phylumentioning

confidence: 99%

Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

Huang

Zhang

et al. 2020

Preprint

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In order to further understand the influence of different temperature conditions in the low temperature range on the microbial community structure in the Anearobic-Anoxic-Oxic waste water treatment process on the plateau, four temperature conditions were designed in the research, including 25°C, 20°C, 15°C and 10°C. Each working condition lasted three days. Activated sludge from anaerobic tank, anoxic tank and oxic tank under each working condition was analyzed based on the 16S rRNA high-throughput sequencing technology. The result shows that the plateau temperature conditions have little influence on the level abundance of phylum. Under all conditions, Bacteroidetes, Proteobacteria and Actinobacteria are the main phyla. The abundance of nitrifying bacterium and phosphorus removal bacteria rose in the first three conditions and fell at 10 °C. The abundance of Denitrification bacteria and Nitrite oxidizing bacteria was significantly higher than that of Ammonia oxidation bacteria and Azotobacter bacteria and Phosphorus-accumulating Organisms(PAO) had an overall advantage over Glycogen-accumulating Organisms (GAO) throughout the research.

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High-temperature EBPR process: The performance, analysis of PAOs and GAOs and the fine-scale population study of Candidatus “Accumulibacter phosphatis”

Cited by 99 publications

References 34 publications

Long term operation of continuous-flow system with enhanced biological phosphorus removal granules at different COD loading

Long term operation of continuous-flow system with enhanced biological phosphorus removal granules at different COD loading

Population Structure and Morphotype Analysis of “ Candidatus Accumulibacter” Using Fluorescence In Situ Hybridization-Staining-Flow Cytometry

Research on the Influence of Different Temperature Conditions on the Microbial Community in Anearobic-Anoxic-Oxic process on the Plateau

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