High-throughput acoustic separation of platelets from whole blood

Chen, Yuchao; Wu, Mengxi; Ren, Liqiang; Liu, Jiayang; Whitley, Pamela; Wang, Lin; Huang, Tony Jun

doi:10.1039/c6lc00682e

Cited by 121 publications

(105 citation statements)

References 59 publications

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“…10,153,154,155,156,157,158,159,160,161,162 Much of this work has focused on developing clever IDT designs, including chirped IDTs that allow for multiple resonance frequencies 10 and tilted-angle IDTs that allow for better control of the separation. 154 One particular advancement was integrating fluorescent labeling and a fluorescence detector to developed a feedback-controlled method for rapid sorting – a so-called fluorescence activated cell sorter (FACS) – that allowed for the selective separation of cell targets from a heterogeneous sample.…”

Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

“…12), and demonstrated the potential for biomarker detection by analyzing the blood from three breast cancer patients at different stages of cancer development. 158 Along those lines, much of the recent focus has been on applying SAW separation to more clinically-relevant conditions, including platelets and bacteria from whole blood 153,159 and inflammatory cells from sputum. 160 While Huang’s group has been the most prolific in this area, others have also contributed 163,164 , demonstrating, for example, SAW-based separation within a surface droplet 165 or using a traveling wave approach, rather than a standing wave.…”

Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

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Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

et al. 2017

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Surface acoustic waves (SAWs), are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for chemical sensing and analysis. The use of SAW techniques for chemical detection in both gaseous and liquid media is discussed, as well as recent fabrication advances that are pointing the way for the next generation of SAW sensors. Similarly, applications and progress in using SAW devices as microfluidic platforms are covered, ranging from atomization and mixing to new approaches to lysing and cell adhesion studies. Finally, potential new directions and perspectives on the field as it moves forward are offered, with a specific focus on potential strategies for making SAW technologies for bioanalytical applications.

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Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

Section: Saw-integrated Microfluidicsmentioning

confidence: 99%

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

et al. 2017

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“…However, its handling capability could be largely enhanced by parallelization or using a slit-shaped microchannel design, with an improved sample throughput up to 3 orders of magnitude in particle separation. 44,45 …”

Section: Resultsmentioning

confidence: 99%

Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows

et al. 2017

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Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30−200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticitybased microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size-dependent, and label-free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.

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“…However, it is now well known that cancer cells can undergo Whole blood sorting, enrichment and in situ labeling N Garg et al epithelial to mesenchymal transition (EMT) in circulation, causing undesired false negatives 41 . Another promising category of microfluidic systems is "label-free", and they are able to sort blood cells based on their sizes using known techniques such as deterministic lateral displacement (DLD) 22,42 , inertial microfluidics 43 , and acoustophoresis 20,21,44 . However, recent developments in oncology have shown heterogeneity and great size overlap between rare cells such as CTCs and healthy WBCs 19,45 .…”

Section: Discussionmentioning

confidence: 99%

Whole-blood sorting, enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming

et al. 2018

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Analyzing undiluted whole human blood is a challenge due to its complex composition of hematopoietic cellular populations, nucleic acids, metabolites, and proteins. We present a novel multi-functional microfluidic acoustic streaming platform that enables sorting, enrichment and in situ identification of cellular subsets from whole blood. This single device platform, based on lateral cavity acoustic transducers (LCAT), enables (1) the sorting of undiluted donor whole blood into its cellular subsets (platelets, RBCs, and WBCs), (2) the enrichment and retrieval of breast cancer cells (MCF-7) spiked in donor whole blood at rare cell relevant concentrations (10 mL − 1 ), and (3) on-chip immunofluorescent labeling for the detection of specific target cellular populations by their known marker expression patterns. Our approach thus demonstrates a compact system that integrates upstream sample processing with downstream separation/enrichment, to carry out multi-parametric cell analysis for blood-based diagnosis and liquid biopsy blood sampling.

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High-throughput acoustic separation of platelets from whole blood

Cited by 121 publications

References 59 publications

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows

Whole-blood sorting, enrichment and in situ immunolabeling of cellular subsets using acoustic microstreaming

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