High-Throughput and Continuous Chaotic Bioprinting of Spatially Controlled Bacterial Microcosms

Ceballos-González, Carlos Fernando; Bolívar-Monsalve, Edna Johana; Quevedo‐Moreno, Diego Alonso; Lam‐Aguilar, Li Lu; Borrayo-Montaño, Karen Ixchel; León, J.; Zhang, Yu Shrike; Álvarez, Mario Moisés; Santiago, Grissel Trujillo‐de

doi:10.1021/acsbiomaterials.0c01646

Cited by 31 publications

(37 citation statements)

References 63 publications

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“…The process of generation of the microstructure during chaotic printing has been detailed in recent reports. ,− Briefly, each KSM element (Figure C) within the printhead sequentially reorients and splits the incoming flow into two streams (Figure B). The two streams of distinct materials originally fed at the top of the printhead are only reoriented in the first element and reoriented and divided into four intercalated layers by the action of the second KSM element.…”

Section: Resultsmentioning

confidence: 99%

Fabrication of Multilayered Composite Nanofibers Using Continuous Chaotic Printing and Electrospinning: Chaotic Electrospinning

Holmberg

Garza-Flores

Almajhadi

et al. 2021

ACS Appl. Mater. Interfaces

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Multi-material and multilayered micro-and nanostructures are prominently featured in nature and engineering and are recognized by their remarkable properties. Unfortunately, the fabrication of micro-and nanostructured materials through conventional processes is challenging and costly. Herein, we introduce a high-throughput, continuous, and versatile strategy for the fabrication of polymer fibers with complex multilayered nanostructures. Chaotic electrospinning (ChE) is based on the coupling of continuous chaotic printing (CCP) and electrospinning, which produces fibers with an internal multi-material microstructure. When a CCP printhead is used as an electrospinning nozzle, the diameter of the fibers is further scaled down by 3 orders of magnitude while preserving their internal structure. ChE enables the use of various polymer inks for the creation of nanofibers with a customizable number of internal nanolayers. Our results showcase the versatility and tunability of ChE to fabricate multilayered structures at the nanoscale at high throughput. We apply ChE to the synthesis of unique carbon textile electrodes composed of nanofibers with striations carved into their surface at regular intervals. These striated carbon electrodes with high surface areas exhibit 3-to 4-fold increases in specific capacitance compared to regular carbon nanofibers; ChE holds great promise for the cost-effective fabrication of electrodes for supercapacitors and other applications.

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Section: Resultsmentioning

confidence: 99%

Fabrication of Multilayered Composite Nanofibers Using Continuous Chaotic Printing and Electrospinning: Chaotic Electrospinning

Holmberg

Garza-Flores

Almajhadi

et al. 2021

ACS Appl. Mater. Interfaces

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“…As an unconventional extrusion method, continuous chaotic printing mixes bioinks through the Kenics static mixer printheads and extrudes defined internal lamellae that exponentially expand the interface areas between the adjacent bioinks. [ 60 ] These methods may prove a promising way to fabricate heterogeneous tissues, such as bone and osteochondral tissue.…”

Section: D Printing Strategiesmentioning

confidence: 99%

Advances in Translational 3D Printing for Cartilage, Bone, and Osteochondral Tissue Engineering

Wang

Zhao

et al. 2022

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The regeneration of 3D tissue constructs with clinically relevant sizes, structures, and hierarchical organizations for translational tissue engineering remains challenging. 3D printing, an additive manufacturing technique, has revolutionized the field of tissue engineering by fabricating biomimetic tissue constructs with precisely controlled composition, spatial distribution, and architecture that can replicate both biological and functional native tissues. Therefore, 3D printing is gaining increasing attention as a viable option to advance personalized therapy for various diseases by regenerating the desired tissues. This review outlines the recently developed 3D printing techniques for clinical translation and specifically summarizes the applications of these approaches for the regeneration of cartilage, bone, and osteochondral tissues. The current challenges and future perspectives of 3D printing technology are also discussed.

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“…These fibers can be used to bioprint defined multi‐material structures featuring large interfacial areas. This KSM technology is growing fast [ 133,134 ] to overcome the limitations of mixing viscous bioinks in passive microfluidic mixers.…”

Section: Multi‐materials Bioprinting Technologiesmentioning

confidence: 99%

Emerging Technologies in Multi‐Material Bioprinting

et al. 2021

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organ-specific tissues, [14] patient-specific grafts, [15] tissue model engineering, [16] and drug screening. [17] The most frequently used technologies for 3D bioprinting include nozzle-based and laser/light-based techniques. Extrusion [18] and inkjet [19] are arguably the most common modalities of nozzle-based bioprinting at the moment, while laser-induced forward transfer (LIFT) [20] and vat-photopolymerization [21] are the two frequently used for laser/lightbased 3D bioprinting. [22] Extrusion bioprinting involves fabrication on a bioprinting platform using bioinks extruded from one or several nozzle(s) (Figure 1A). The extrusion process may be pressure-controlled, with the bioink entrained by means of pneumatic actuation, or flow rate-controlled with the bioink forced by mechanical impulses through syringes. [23] Solidification of the bioprinted structures as they are delivered is obtained through physical, chemical, or photo-crosslinking. [24] Extrusion bioprinting is relatively inexpensive, straightforward, and convenient. It has been embodied, for example, within handheld and portable devices. [25][26][27][28][29] But such convenience must be traded off against significant challenges. Nozzle extrusion necessarily entails a high level of shear stress near the fluidic channel walls. Excessive shear, particularly in high-viscosity bioinks, jeopardizes cell viability. [30] High-resolution bioprinting often requires small-diameter nozzles. The greater shear required imposes a limit on bioink flow rate and throughput. This problem may be addressed through the use of printable shearthinning biomaterials, [31] for which the viscosity decreases under shear stress. Yet, shear-thinning bioink materials that meet all design requirements are not always available. Further notable challenges of extrusion methods include difficulties in finding stable in situ crosslinking methods for nonshear-thinning bioinks, as well as low printing resolution [32] in relation to other methods, and complications in the fabrication of freestanding constructs. [33,34] These shortcomings have spurred many enhancements, notably co-axial/core-shell bioprinting, [35] described in Section 3.1.3, and embedded bioprinting, [36] in Section 3.1.4.Inkjet bioprinting (Figure 1B), similar to home/office inkjet printing, delivers small droplets of bioink to a substrate and can produce high-resolution voxelated constructs. [37] Unlike the extrusion method, where shear-thinning bioinks with a broad range of viscosities can be used, inkjet bioprinters are mainly designed to work with low-viscosity bioinks. [38] The deposition of droplets/voxels is controlled either by thermal, piezoelectric, Bioprinting, within the emerging field of biofabrication, aims at the fabrication of functional biomimetic constructs. Different 3D bioprinting techniques have been adapted to bioprint cell-laden bioinks. However, single-material bioprinting techniques oftentimes fail to reproduce the complex compositions and diversity of native tissues. Multi-material bioprinting as...

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High-Throughput and Continuous Chaotic Bioprinting of Spatially Controlled Bacterial Microcosms

Cited by 31 publications

References 63 publications

Fabrication of Multilayered Composite Nanofibers Using Continuous Chaotic Printing and Electrospinning: Chaotic Electrospinning

Fabrication of Multilayered Composite Nanofibers Using Continuous Chaotic Printing and Electrospinning: Chaotic Electrospinning

Advances in Translational 3D Printing for Cartilage, Bone, and Osteochondral Tissue Engineering

Emerging Technologies in Multi‐Material Bioprinting

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