High-throughput droplet analysis and multiplex DNA detection in the microfluidic platform equipped with a robust sample-introduction technique

Chen, Jinyang; Ji, Xinghu; He, Zhibing

doi:10.1016/j.aca.2015.07.054

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…Moreover, microfluidic systems are useful for highthroughput screening of cells, 28−30 proteins, 31,32 and DNA. 33,34 In particular, microfluidic systems are suitable for monitoring the tactic responses of microorganisms to various stimuli because microfluidics enables precise control of fluid properties without the influence of external environmental factors. 35 For these reasons, microfluidic systems can be extremely useful in analyzing tactic responses.…”

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confidence: 99%

“…However, these methods have limitations in accurately quantifying the tactical response and stably supplying the stimuli that cause tactic responses of cells. , Currently, microfluidic technologies have emerged as promising analytical tools in various biological fields ,− due to their numerous advantages over conventional analytical techniques, such as small sample volume, short analysis time, low cost, and high sensitivity. Moreover, microfluidic systems are useful for high-throughput screening of cells, − proteins, , and DNA. , In particular, microfluidic systems are suitable for monitoring the tactic responses of microorganisms to various stimuli because microfluidics enables precise control of fluid properties without the influence of external environmental factors . For these reasons, microfluidic systems can be extremely useful in analyzing tactic responses …”

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confidence: 99%

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Two-Dimensional Microfluidic System for the Simultaneous Quantitative Analysis of Phototactic/Chemotactic Responses of Microalgae

et al. 2018

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Microalgae have been spotlighted as a renewable energy source to produce biofuels from CO2 by photosynthesis. However, their innate inefficiency of CO2 conversion using light energy has been a challenge to the commercialization of algae-based biofuel production. Photosynthetic organisms have evolved behavioral responses, including phototaxis and chemotaxis, to find optimal conditions for capturing light energy and inorganic carbon (Ci) sources for photosynthesis. In this context, investigation of phototaxis and chemotaxis to HCO3 –, the predominant form of Ci in neutral aqueous solutions, is necessary to understand the physiological role of tactic responses in photosynthesis. In this study, a two-dimensional microfluidic system enabled efficient analysis of phototactic and chemotactic responses by investigation of cell distribution in the outlet chambers. From statistical analysis (skewness and kurtosis) of tactic responses of different algal strains to external stimuli, the preferred concentrations of HCO3 – for Chlamydomonas reinhardtii CC125 (wild type), PTS42 (random insertional mutant of C. reinhardtii, high photosynthetic activity), and CC2702 (cia5 mutant of C. reinhardtii, unable to acclimate to low CO2 concentration) were determined to be 27.22, 43.23, and 36.95 mM, respectively. From the analysis of tactic responses of wild type and 14 mutant strains, it was found that the photosystem II (PSII) operating efficiency and CO2 fixation rate were strongly correlated with the phototactic (R 2 = 0.931) and chemotactic response (R 2 = 0.857), respectively. Finally, this system can be applied to high-throughput screening strategies for the rapid isolation of high photosynthetically productive microalgal strains based on their tactic responses.

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confidence: 99%