High throughput easy microinjection with a single-cell manipulation supporting robot

Matsuoka, Hiroaki; Komazaki, Tamu; Mukai, Yoshiko; Shibusawa, Meiri; Akane, Hirotoshi; Chaki, Akihiko; Uetake, Norio; Saito, Mikako

doi:10.1016/j.jbiotec.2004.10.010

Cited by 75 publications

(32 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microinjection techniques are widely used in cell biology when one needs to introduce such substances into the cytoplasm of living cells (1). Important advantages of microinjection over other techniques, such as lipofection (2) and electroporation (3), include the abilities to target specific cells and inject practically any material into the cell with some degree of spatial selectivity (4).…”

mentioning

confidence: 99%

Electrochemical attosyringe

Laforge

Carpino

Rotenberg

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

166

186

View full text Add to dashboard Cite

The ability to manipulate ultrasmall volumes of liquids is essential in such diverse fields as cell biology, microfluidics, capillary chromatography, and nanolithography. In cell biology, it is often necessary to inject material of high molecular weight (e.g., DNA, proteins) into living cells because their membranes are impermeable to such molecules. All techniques currently used for microinjection are plagued by two common problems: the relatively large injector size and volume of injected fluid, and poor control of the amount of injected material. Here we demonstrate the possibility of electrochemical control of the fluid motion that allows one to sample and dispense attoliter-to-picoliter (10 ؊18 to 10 ؊12 liter) volumes of either aqueous or nonaqueous solutions. By changing the voltage applied across the liquid/liquid interface, one can produce a sufficient force to draw solution inside a nanopipette and then inject it into an immobilized biological cell. A high success rate was achieved in injections of fluorescent dyes into cultured human breast cells. The injection of femtoliter-range volumes can be monitored by video microscopy, and current/resistance-based approaches can be used to control injections from very small pipettes. Other potential applications of the electrochemical syringe include fluid dispensing in nanolithography and pumping in microfluidic systems.liquid/liquid interface ͉ microinjection ͉ nanopipette ͉ fluid delivery ͉ nanopump

show abstract

mentioning

confidence: 99%

Electrochemical attosyringe

Laforge

Carpino

Rotenberg

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

166

186

View full text Add to dashboard Cite

show abstract

“…We chose ES cells in this study because they have stickier cell membrane than the other cells such as HeLa cells [8]. The EB3 ES cells were provided by Dr. Hitoshi Niwa (RIKEN) [9,10], and cultured at 37…”

Section: Experimental Methodsmentioning

confidence: 99%

Coating the Outer Surface of Glass Nanopipette with Chlorobenzene-Terminated Polysiloxane

Takami

Ojiro

Ogawa

et al. 2015

e-J. Surf. Sci. Nanotech.

Self Cite

View full text Add to dashboard Cite

A method of outer surface modification of glass nanopipette with chlorobenzene-terminated organopolysiloxane has been developed. Scanning electron microscope images and microscopic Raman spectra revealed the efficacy of the coating. Energy dispersive X-ray spectra showed not only the materials adsorbed on the nanopipette but also the change in stoichiometry of the bulk glass resulting from the fabrication process of the nanopipette with a laser puller. The coating method is easy to treat and can be used for various applications such as the prevention of carbon contamination from the materials during the injection to biomaterials such as living cells.

show abstract

“…A 0.9 g cm ¹3 LYsolution or a 0.8 mol cm ¹3 DTRsolution was filled in a glass capillary. By means of a singlecell manipulation supporting robot, [21][22][23] the capillary was injected into a target HeLa single-cell that expressed Cx43-TC and the dye solution was introduced into the cell under a condition of 0.7 kg cm ¹2 for 10 ms. Then the cells were observed with a fluorescent microscope.…”

Section: Femtoinjection and Fluorescence Analysismentioning

confidence: 99%

“…Such an experiment could be successfully conducted by means of a femtoinjection system. [21][22][23] 2. Experimental…”

Section: Introductionmentioning

confidence: 99%

A Tetracysteine-tag and HeLa Cell System for the Dynamic Analysis of the Localization and Gating Properties of a Specific Connexin Isoform

2016

Self Cite

View full text Add to dashboard Cite

Cell-cell communication in animals is predominantly conducted via gap junction (GJ) plaque comprising 20 connexin (Cx) isoforms. However the GJ plaques are formed at only limited part of cell-cell interface and such an irregular localization is a current problem. To solve this problem, we developed an experimental platform to analyze the role of each Cx isoform in the regulation of GJ plaque localization and in the intercellular molecular movement. A tetracysteine-tagged Cx expressed in HeLa cell deficient in Cx was found feasible for viable imaging of GJ plaques. Femtoinjection enabled the quantitative analysis of intercellular dye diffusion.

show abstract

High throughput easy microinjection with a single-cell manipulation supporting robot

Cited by 75 publications

References 25 publications

Electrochemical attosyringe

Electrochemical attosyringe

Coating the Outer Surface of Glass Nanopipette with Chlorobenzene-Terminated Polysiloxane

A Tetracysteine-tag and HeLa Cell System for the Dynamic Analysis of the Localization and Gating Properties of a Specific Connexin Isoform

Contact Info

Product

Resources

About