High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

Yang, Wenguang; Yu, Haibo; Li, Gongxin; Wang, Yuechao; Liu, Lianqing

doi:10.1002/smll.201602769

Cited by 73 publications

(52 citation statements)

References 55 publications

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“…Point-shaped cell-laden structures that contain different types of cells can be precisely arranged within macroscopic tissues by applying microfluidic and dielectrophoretic forces in microchannels in a high-throughput manner. This approach enables the spatial control of co-culture of different tissues 117,118 (FIG. 5b,c).…”

Section: In Vitro 3d Modelsmentioning

confidence: 99%

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Biofabrication strategies for 3D in vitro models and regenerative medicine

et al. 2018

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Organs are complex systems composed of different cells, proteins and signalling molecules that are arranged in a highly ordered structure to orchestrate a myriad of functions in our body. Biofabrication strategies can be applied to engineer 3D tissue models in vitro by mimicking the structure and function of native tissue through the precise deposition and assembly of materials and cells. This approach allows the spatiotemporal control over cell-cell and cell-extracellular matrix communication and thus the recreation of tissue-like structures. In this Review, we examine biofabrication strategies for the construction of functional tissue replacements and organ models, focusing on the development of biomaterials, such as supramolecular and photosensitive materials, that can be processed using biofabrication techniques. We highlight bioprinted and bioassembled tissue models and survey biofabrication techniques for their potential to recreate complex tissue properties, such as shape, vasculature and specific functionalities. Finally, we discuss challenges, such as scalability and the foreign body response, and opportunities in the field and provide an outlook to the future of biofabrication in regenerative medicine.

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Section: In Vitro 3d Modelsmentioning

confidence: 99%

“…Panel a is reproduced from REF 117 , Macmillan Publishers Limited. Panel b is adapted with permission from REF 118 , John Wiley and Sons. Panel c is adapted from REF 121 , Macmillan Publishers Limited.…”

Section: Fig 1 |mentioning

confidence: 99%

Biofabrication strategies for 3D in vitro models and regenerative medicine

et al. 2018

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“…Remote assembly techniques use force fields to interact and manipulate matter in a non-contact fashion, an approach that can be used for modular tissue engineering. While acknowledging that dielectrophoresis [197] and thermal convection [198] have also been used to manipulate living building blocks, here we focus on the three major categories of remote field manipulation: acoustic, magnetic and optical assembly ( Figure 1C, Table 2). [199] Generally, these techniques exploit physical differences between living building blocks and the surrounding medium (e.g., density, compressibility, refractive index, paramagnetism) to exert forces that can be used to remotely assemble different tissue engineering components.…”

Section: Remote Assembly Of Living Building Blocksmentioning

confidence: 99%

Assembling Living Building Blocks to Engineer Complex Tissues

Ouyang

Armstrong

Salmerón‐Sánchez

et al. 2020

Adv Funct Materials

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The great demand for tissue and organ grafts, compounded by an ageing demographic and a shortage of available donors, has driven the development of bioengineering approaches that can generate biomimetic tissues in vitro. Despite the considerable progress in conventional scaffold-based tissue engineering, the recreation of physiological complexity has remained a challenge. Bottom-up tissue engineering strategies have opened up a new avenue for the modular assembly of living building blocks into customized tissue architectures. This Progress Report will overview the recent progress and trends in the fabrication and assembly of living building blocks, with a key highlight on emerging bioprinting technologies that can be used for modular assembly and complexity in tissue engineering. By summarizing the work to date, providing new classifications of different living building blocks, highlighting state-of-the-art research and trends, and offering personal perspectives on future opportunities, this Progress Report aims to aid and inspire other researchers working in the field of modular tissue engineering.

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“…Advances in micromanipulation technology provide us an important tool in industry, particularly in the assembly of micro‐optoelectronic devices and tests of micro components . They also enable us to manipulate single biological cells including the cell measurement, assembly, injection, and enucleation . In micromanipulation, immobilization, transportation, and rotation are the three fundamental operations.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional Noncontact Micromanipulation Using Whirling Flow Generated by Vibrating a Single Piezo Actuator

Liu

Shi

Lin

et al. 2018

Small

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A noncontact method that can achieve immobilization, transportation, and rotation in the microscale is desired in biological micromanipulation. A multifunctional noncontact micromanipulation method is proposed here based on a vibration‐generated whirling flow. Resonance of a cantilever structure is utilized to extend the straight vibration of a single piezo actuator to the 2D circular vibration of a micropipette. The circular vibration in fluids can generate the whirling flow featured with low pressure in the core area and flow velocity gradient. The low pressure can immobilize the objects nearby and transport them together with the micropipette, and the flow velocity gradient is utilized to form a torque to rotate the immobilized object. Experiments of the microbeads are conducted to evaluate the claimed functions and quantify the key parameters that influence the rotation velocity. The cell spheroid is immobilized and rotated for 3D observation, and by assessing the viability of the cells containing in the spheroid, the proposed method is proved noninvasive to living cells. Finally, another important application in operations of mouse egg cells is shown, which indicates that the proposed method is a potential valuable tool in biological micromanipulation.

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High‐Throughput Fabrication and Modular Assembly of 3D Heterogeneous Microscale Tissues

Cited by 73 publications

References 55 publications

Biofabrication strategies for 3D in vitro models and regenerative medicine

Biofabrication strategies for 3D in vitro models and regenerative medicine

Assembling Living Building Blocks to Engineer Complex Tissues

Multifunctional Noncontact Micromanipulation Using Whirling Flow Generated by Vibrating a Single Piezo Actuator

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