High-Throughput Metabolomics Evaluate the Efficacy of Total Lignans From Acanthophanax Senticosus Stem Against Ovariectomized Osteoporosis Rat

Abstract

Postmenopausal osteoporosis (PMOP) is a common clinical illness in postmenopausal women, but there is no effective drug at present. Metabolomics approach was used to explore the potential biomarkers of PMOP and evaluate the efficacy and therapeutic targets of total lignans in the stem of Acanthophanax senticosus (ASSL) on the ovariectomized osteoporosis model rats. UPLC/MS and pattern recognition methods were used for serum metabolites discovery to illustrate the pathological mechanism of PMOP model rats, and then revealing the intervention effect of ASSL. The pattern recognition result showed that serum metabolic profiles of the sham operation group and the model group were clustered clearly, and 16 potential biomarkers were finally identified (7 in positive ion mode and 9 in negative ion mode), and they are involved in 15 related metabolic pathways. After oral administration of ASSL, 10 biomarkers were found to be significantly up-regulated and mainly regulated metabolic pathways include unsaturated fatty acid biosynthesis, linoleic acid metabolism, and arachidonic acid metabolism, primary bile acid synthesis, tyrosine metabolism, etc. Our study demonstrated that the ASSL could affect the endogenous metabolites related metabolic mechanism, provides a pharmacological basis of the ASSL for PMOP treatment.

Comparative metabolomics unveils molecular changes and metabolic networks of syringin against hepatitis B mice by untargeted mass spectrometry

“…99 Currently, the detection of serum biomarkers can be used as an indicator to reect disease states. [100][101][102][103][104][105][106][107][108][109][110][111][112][113][114][115] Syringin can regulate the levels of proline, ornithine, and glutamate to the level of control group by affecting alanine, aspartate and glutamate metabolism, and arginine and proline metabolism.…”

Metabolomics biotechnology, applications, and future trends: a systematic review

“…Among them, the NMR technique generally uses a hydrogen spectrum ( 1 H NMR), a carbon spectrum ( 13 C NMR), or a phosphorus spectrum ( 31 P NMR), of which 1 H NMR is the most widely used. [6][7][8][9] Metabolomics research is still a complete explanation of the data. Because the original map of metabolomics research is complex and the data volume is large, it cannot be analyzed by conventional data processing methods, so metabolomics analysis involves a special analysis with three steps: data extraction, data preprocessing and pattern recognition.…”

Characterization of chemical constituents and absorbed components, screening the active components of gelanxinning capsule and an evaluation of therapeutic effects by ultra‐high performance liquid chromatography with quadrupole time of flight mass spectrometry

“…With traditional medicines comes onto a new worldwide arena in clinical medicine and pharmaceutical research; natural products are becoming popular over the world and widely accepted as a conventional clinic therapy compared with currently available therapeutic options [6][7][8][9][10][11]. So far, much work has been focused on the major role played by herbs for health promotion and various diseases prevention, management, treatment due to their effectiveness, convenience, less side effects, and relatively low cost [12][13][14][15][16][17][18][19]. Traditional medicines exist in complex situations such as multiple sources and producing area leading to the obvious difference of traditional medicines quality, especially the amount of effective ingredients show significantly different [20][21][22][23][24][25][26][27].…”