High-throughput microfluidic system for long-term bacterial colony monitoring and antibiotic testing in zero-flow environments

Sun, Peng; Liu, Yang; Sha, Jun; Zhang, Zhiyun; Tu, Qin; Chen, Peng; Wang, Jinyi

doi:10.1016/j.bios.2010.08.062

Cited by 64 publications

(39 citation statements)

References 33 publications

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“…29 Several rapid, microfluidic methods for antibiotic testing have been proposed, including easy handling devices with colorimetric readout, 18,38 analysis of dielectrophoretic behavior, 39 polymerase chain reaction, 40 and continuous flow chips with immobilized samples. 16,[41][42][43][44] As recently stated by Whitesides 45 and Chin et al, 46 a more general acceptance of microfluidic devices requires simple methodology and handling as well as alternative fabrication methods. Sacrificing oxygen permeability of PDMS-based devices for advantageous handling, priming, and fabrication requires an alternative solution for oxygenation.…”

Section: Introductionmentioning

confidence: 92%

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Hydrogel-based microfluidic incubator for microorganism cultivation and analyses

et al. 2015

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This work presents an array of microfluidic chambers for on-chip culturing of microorganisms in static and continuous shear-free operation modes. The unique design comprises an in-situ polymerized hydrogel that forms gas and reagent permeable culture wells in a glass chip. Utilizing a hydrophilic substrate increases usability by autonomous capillary priming. The thin gel barrier enables efficient oxygen supply and facilitates on-chip analysis by chemical access through the gel without introducing a disturbing flow to the culture. Trapping the suspended microorganisms inside a gel well allows for a much simpler fabrication than in conventional trapping devices as the minimal feature size does not depend on cell size. Nutrients and drugs are provided on-chip in the gel for a self-contained and user-friendly handling. Rapid antibiotic testing in static cultures with strains of Enterococcus faecalis and Escherichia coli is presented. Cell seeding and diffusive medium supply is provided by phaseguide technology, enabling simple operation of continuous culturing with a great flexibility. Cells of Saccharomyces cerevisiae are utilized as a model to demonstrate continuous on-chip culturing.

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Section: Introductionmentioning

confidence: 92%

“…Devices for shear-free cultures make use of dead-end growing chambers with diffusive medium supply from a microfluidic channel. Seeding strategies, including injection through the chip cover, 15 high pressure, 16 or application of a vacuum, 17,18 can be complex, while harvesting of cells after an experiment is hardly possible.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel-based microfluidic incubator for microorganism cultivation and analyses

et al. 2015

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“…1) was fabricated from PDMS, an optically transparent elastomer widely used in biological microfluidics (Sun et al 2011;Thorsen et al 2002). Pneumatically actuated valves were employed to allow the manipulation of individual cell culture chambers and the spatiotemporally controlled investigation of the interaction between MDA231-LM2 and MDA-MB231 cells (Thorsen et al 2002;Zheng et al 2012).…”

Section: Device Design and Fabricationmentioning

confidence: 99%

Microvalve and liquid membrane double-controlled integrated microfluidics for observing the interaction of breast cancer cells

Liu

Wang

et al. 2012

Microfluid Nanofluid

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The methodical development of cell biology has resulted in significant advancements in the study of breast tumors. However, the dynamic investigation of the self-seeding process remains largely out of reach. In the present study, we describe a microvalve and liquid membrane double-controlled integrated microfluidic device that provides for the versatile assessment of breast tumor cell invasion dynamics. The liquid membrane formation was first optimized to obtain a high level of control, and was then applied to different types of homotypic and heterotypic cell seeding with precise selective positioning for monoculture and coculture. Using this device, the interaction between breast cancer cells MDA231-LM2 and MDA-MB231 was successfully observed to investigate self-seeding dynamics, including migration, infiltration, and coexistence. The results quantitatively demonstrate the mutual signal-induced attraction between MDA-MB231 and MDA231-LM2 cells, as well as the progressive infiltration of MDA231-LM2 cells into the MDA-MB231 cell population. These results are valuable in the development of spatiotemporal-controlled microfluidic systems and to many microscale-based biological and diagnostic studies involving cell growth, cell differentiation, cell interaction, and cell signal.

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“…However, certain highly environment-sensitive cell types such as neuron are always maintained and cultured in the static or half-perfusion microenvironment of the devices (Hosmane et al, 2010). Meanwhile, diffusion is likewise used for the suspension culture of cells such as bacteria (Escherichia coli) and plant cells (tobacco mesophyll protoplasts) Sun et al, 2011), which requires greater control in the devices due to non-physical dependence. Fig.…”

Section: Cell Culturementioning

confidence: 99%

Microfluidics-Based Cell Manipulation and Analysis

Wang¹,

Liu²,

Li³

et al. 2011

On Biomimetics

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High-throughput microfluidic system for long-term bacterial colony monitoring and antibiotic testing in zero-flow environments

Cited by 64 publications

References 33 publications

Hydrogel-based microfluidic incubator for microorganism cultivation and analyses

Hydrogel-based microfluidic incubator for microorganism cultivation and analyses

Microvalve and liquid membrane double-controlled integrated microfluidics for observing the interaction of breast cancer cells

Microfluidics-Based Cell Manipulation and Analysis

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