High-throughput multi-gate microfluidic resistive pulse sensing for biological nanoparticle detection

Kim, June Soo; Kwon, Sang Hoon; Lee, Jae Yong; Kim, Seung Deok; Kim, Da Ye; Kim, Hyun-Jun; Jang, Noah; Wang, Jiajie; Han, Maeum; Kong, Seong Ho

doi:10.1039/d2lc01064j

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…It is crucial to achieve sensitive and accurate cell detection results in MIC [9]. Various optimization techniques have been developed to enhance the performance of MIC platforms for bio-particle event detection, including improving the electrode layout [10][11][12][13], channel design [14][15][16][17], and data processing algorithms [18,19]. These optimizations aim to reduce the baseline noise, decrease the coefficient of variation (CV) of events, increase the signal-tonoise ratio (SNR) of particle events, and improve the detection capability of the MIC system.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Microfluidic Impedance Cytometry by Bypass Electrode Layout Design

Wu,

Zhang,

et al. 2024

Biosensors

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Microfluidic impedance cytometry (MIC) has emerged as a popular technique for single-cell analysis. Traditional MIC electrode designs consist of a pair of (or three) working electrodes, and their detection performance needs further improvements for microorganisms. In this study, we designed an 8-electrode MIC device in which the center pair was defined as the working electrode, and the connection status of bypass electrodes could be changed. This allowed us to compare the performance of layouts with no bypasses and those with floating or grounding electrodes by simulation and experiment. The results of detecting Φ 5 μm beads revealed that both the grounding and the floating electrode outperformed the no bypass electrode, and the grounding electrode demonstrated the best signal-to-noise ratio (SNR), coefficient of variation (CV), and detection sensitivity. Furthermore, the effects of different bypass grounding areas (numbers of grounding electrodes) were investigated. Finally, particles passing at high horizontal positions can be detected, and Φ 1 μm beads can be measured in a wide channel (150 μm) using a fully grounding electrode, with the sensitivity of bead volume detection reaching 0.00097%. This provides a general MIC electrode optimization technology for detecting smaller particles, even macromolecular proteins, viruses, and exosomes in the future.

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Section: Introductionmentioning

confidence: 99%

Optimizing Microfluidic Impedance Cytometry by Bypass Electrode Layout Design

Wu,

Zhang,

et al. 2024

Biosensors

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“…Single-nanoparticle detection is important for life sciences, 1,2 biomedical research, 3–5 and environmental monitoring. 6 For instance, the reliable and rapid detection of infectious viruses ( e.g.…”

Section: Introductionmentioning

confidence: 99%

An optical nanofibre-enabled on-chip single-nanoparticle sensor

Liu,

Yao,

Wang

et al. 2023

Lab Chip

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“…In this work, the orifice of a laserpulled glass nanopipette is used to resistively sense the translocations of liposomes as they enter and leave the nanopipette. Recent biological applications of resistive-pulse sensing include the characterization of exosomes originating from the MDA-MB-231 breast cancer cell line, 27,28 delivery of liposomes containing redox active species to electrode surfaces, 29,30 deformation measurements of multilamellar liposomes 31 and pseudo-type human immunodeficiency virus (HIV-1), 32 and characterization of DNA. 33,34 The characterization of biologically relevant particles via resistive-pulse sensing generally has limited sizing precision when translocating the particle one time through an opening separating two electrolyte solutions.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

Schmeltzer,

Peterson,

Lathrop

et al. 2023

Preprint

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Simultaneous multipass resistive-pulse sensing and fluorescence imaging have been used to correlate the size and fluorescence intensity of individual E. coli lipid liposomes composed of E. coli polar lipid extract labeled with membrane-bound 3,3-dioctadecyloxacarbocyanine (DiO) fluorescent molecules. Here, a nanopipette serves as a waveguide to direct excitation light to the resistive-pulse sensing zone at the end of the nanopipette tip. Individual DiO-labeled liposomes (>50 nm radius) were multipassed back and forth through the orifices of glass nanopipettes 110-to-150 nm radius via potential switching to obtain sub-nanometer sizing precision, while recording the fluorescence intensity of the membrane-bound DiO molecules. Fluorescence was measured as a function of liposome radius and found to be approximately proportional to the total membrane surface area. The observed relationship between liposome size and fluorescence intensity suggests that multi-vesicle liposomes emit greater fluorescence compared to unilamellar liposomes, consistent with all lipid membranes of the multi-vesicle liposomes containing DiO. Fluorescent and non-fluorescent liposomes are readily distinguished from each other in the same solution using simultaneous multipass resistive-pulse sensing and fluorescence imaging. A fluorescence ‘dead zone’ of ~1 m thickness just outside of the nanopipette orifice was observed during resistive-pulse sensing, resulting in ‘on/off’ fluorescent behavior during liposome multipassing. Our results provide a path forward to simultaneously characterize the size of biologically relevant nanoparticles (e.g., extracellular vesicles) and the presence of fluorescently labeled surface proteins.

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High-throughput multi-gate microfluidic resistive pulse sensing for biological nanoparticle detection

Abstract: We presented microfluidic resistive pulse sensing for submicron particles and exosomes with high sensitivity via multiple gates and gate structure modification.

Cited by 8 publications

References 42 publications

Optimizing Microfluidic Impedance Cytometry by Bypass Electrode Layout Design

Optimizing Microfluidic Impedance Cytometry by Bypass Electrode Layout Design

An optical nanofibre-enabled on-chip single-nanoparticle sensor

Simultaneous Multipass Resistive-Pulse Sensing and Fluorescence Imaging of Liposomes

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