High Throughput, Parallel Imaging and Biomarker Quantification of Human Spermatozoa by ImageStream Flow Cytometry

Buckman, Clayton; George, Thaddeus C.; Friend, Sherree; Sutovsky, Miriam; Miranda‐Vizuete, Antonio; Ozanon, Christophe; Morrissey, Phil; Šutovský, Peter

doi:10.3109/19396360903056224

Cited by 32 publications

(30 citation statements)

References 18 publications

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“…The novel ImageStream technology is combining FC with cell imaging parallel to quantification and visualization of spermatid-specific thioredoxin-3 protein in defective spermatozoa. 180 Cytometry imaging with charge-coupled device camera facilitate image of sperm, while they pass the laser beam. The possibilities of this new FC for sperm evaluation are infinite.…”

Section: Future Perspectives For Fc In Spermatologymentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

143

120

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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Section: Future Perspectives For Fc In Spermatologymentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

143

120

View full text Add to dashboard Cite

show abstract

“…Sperm cells are complex models, and the study of their motility is necessary in order to describe the quality of the samples [1], which is important in the fertilization process as faster and quantitative analysis help to identify the best sample; in recent years, computational systems have been used to measure the quality of human spermatozoa [2], and for following morphological changes [3]. Semen is manipulated in vitro at the industrial level in order to improve production.…”

Section: Introductionmentioning

confidence: 99%

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Parodí

Ramirez-Reveco²,

Guerra³

2015

ABB

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A spermiogramme is an analysis performed to assess the quality of semen. Motility parameters are primarily obtained by subjectively observing samples or by using complex systems, such as computer-assisted sperm analysis (CASA). Here, we describe an easy and low-cost analysis system for obtaining quantifiable kinetic observations using Salmo salar semen model. In this work, we observed and captured video images of both fresh and stored Atlantic salmon semen to describe the possibility of analysis using the ImageJ CASA plug-in application for the kinetic parameters obtained from the videos. The semen is exposed to "powermilt", a commercial activating solution, and the curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP) values are described. When the samples were activated after having been stored, differences were detected in sperm quality, using the low-cost plug-in application. However, this system was not able to detect small variations in the same recorded sample, suggesting limits in sample observation. The results indicate that it is possible to quantify the kinetic parameters of semen samples using a low-cost video system and free software.

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“…This methodology has been applied to dog, boar, and stallion semen (57-59) as well as to human (60). A new development in the area is that of image stream analysis that incorporates both imaging and flow cytometry (61).…”

Section: Morphologymentioning

confidence: 99%

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

Petrunkina

Harrison²

2011

Cytometry Pt A

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Cytometric methodologies are becoming increasingly important in veterinary andrology as means of assessing sperm function. However, as yet, flow cytometric techniques in veterinary andrology have not kept up in sophistication with those in other areas of biology and medicine. In this brief review, we consider the present state of cytometry in andrological procedures for evaluating the fertility of domestic animal sires. We outline the aspects of sperm physiology, paying particular attention to the changes that take place during the process known as capacitation, which prepares the sperm for interaction with the egg. We then examine briefly but critically the cytometric techniques that are currently in commercial use or are being established in research laboratories for testing sperm characteristics. Current limitations and potential developments in semen assessment are discussed. Recent research knowledge offers possibilities for applying more subtle flow cytometric approaches to distinguish different levels of fertilizing potential in semen samples. For example, linking field fertility data to multiparametric kinetic studies of sperm capacitational changes rather than ''single parameter-single time point'' estimations may reveal that slower rather than rapid changes indicate high fertility. Moreover, the development of multicolor flow cytometric procedures as a means of evaluating multiple functional parameters in individual cells would reduce the uncertainties always inherent in predicting fertility from in vitro sperm evaluation tests. ' 2011 International Society for Advancement of Cytometry Key terms fertility; sperm evaluation; heterogeneity; subpopulations; multicolour cytometry THE main focus of an andrological assessment in veterinary medicine is to ascertain the fertility of a potential sire. Although the most obvious and indeed frequently used general veterinary test is to quantify the number of offspring born to the sire, this is a lengthy and very expensive process. For ''accuracy,'' many inseminations must be carried out, and results must await gestation and birth. Obviously, therefore, development of rapid tests that can be carried out in the laboratory has for long been important. However, as research into the reproductive process has advanced, it has become clear that the life of the fertilizing sperm between its release from the testis and its fusion with the egg is far from simple. Moreover, the population of sperm ejaculated into the female has been revealed as being heterogeneous in functional ability, even if morphologically normal [c.f. reviews (1,2)]. As a result of these findings, tests are being developed to examine functional ability within a heterogeneously responding population (3-5).Given that a normal male ejaculate contains many millions of individual sperm cells, it is natural that these tests are based on cytometry. In this short review, we will outline and comment upon a range of modern and still-developing cytometric methodologies in use or potentially useful for sperm...

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High Throughput, Parallel Imaging and Biomarker Quantification of Human Spermatozoa by ImageStream Flow Cytometry

Cited by 32 publications

References 18 publications

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

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High Throughput, Parallel Imaging and Biomarker Quantification of Human Spermatozoa by ImageStream Flow Cytometry

Cited by 32 publications

References 18 publications

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (&lt;i&gt;Salmo salar&lt;/i&gt;)

Cytometric solutions in veterinary andrology: Developments, advantages, and limitations

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Product

Resources

About

Example Use of Low-Cost System for Capturing the Kinetic Parameters of Sperm Cells in Atlantic Salmon (<i>Salmo salar</i>)