2011
DOI: 10.1016/j.sbi.2011.07.001
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High throughput platforms for structural genomics of integral membrane proteins

Abstract: Structural genomics approaches on integral membrane proteins have been postulated for over a decade, yet specific efforts are lagging years behind their soluble counterparts. Indeed, high throughput methodologies for production and characterization of prokaryotic integral membrane proteins are only now emerging, while large-scale efforts for eukaryotic ones are still in their infancy. Presented here is a review of recent literature on actively ongoing structural genomics of membrane protein initiatives, with a… Show more

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Cited by 27 publications
(18 citation statements)
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“…As a natural prerequisite, the protein needs to be expressed in one of a number of heterologous expression systems, such as Escherichia coli , yeasts, insect cells or mammalian cells [2] . A number of expression strategies have been developed for each host system and many are now efficient for expression trials of hundreds of proteins in parallel [3] . A popular strategy for the expression of membrane proteins in E. coli is to generate fusion proteins with green fluorescent protein (GFP), which can be used as an indicator for both the quantity of protein expressed [4] and its relative stability upon detergent solubilisation by fluorescence-detection size-exclusion chromatography (FSEC) [5] .…”
Section: Introductionmentioning
confidence: 99%
“…As a natural prerequisite, the protein needs to be expressed in one of a number of heterologous expression systems, such as Escherichia coli , yeasts, insect cells or mammalian cells [2] . A number of expression strategies have been developed for each host system and many are now efficient for expression trials of hundreds of proteins in parallel [3] . A popular strategy for the expression of membrane proteins in E. coli is to generate fusion proteins with green fluorescent protein (GFP), which can be used as an indicator for both the quantity of protein expressed [4] and its relative stability upon detergent solubilisation by fluorescence-detection size-exclusion chromatography (FSEC) [5] .…”
Section: Introductionmentioning
confidence: 99%
“…Recently published high-throughput insect cell expression systems have streamlined expression screening and protein analysis, but still adhere to generating and amplifying recombinant baculoviruses for each individual construct (Mancia and Love, 2011;Hanson et al, 2007;Buchs et al, 2009). In Fig.…”
Section: Introductionmentioning
confidence: 99%
“…This leaves ~ 6000 insoluble proteins with structures yet to be explored. While it is potentially possible to crystallize membrane proteins via the use of either detergents or lipid cubic phases, at the moment high-output platforms for determination of integral membrane protein structures by X-ray crystallography are still far from optimal [43,44], and the selection of detergents for protein production and successful crystallization of membrane proteins remains highly challenging.…”
Section: Macromolecular X-ray Crystallography: the Past Current Amentioning
confidence: 99%