High‐throughput quantification of perindopril in human plasma by liquid chromatography/tandem mass spectrometry: application to a bioequivalence study

Abstract

A simple, sensitive and rapid high-performance liquid chromatography/positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of perindopril in human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reversed-phase column and analyzed by mass spectrometry in the multiple reaction monitoring mode using the respective [M+H](+) ions, m/z 369/172 for perindopril and m/z 417/234 for the internal standard. The method exhibited a linear dynamic range of 0.1-100 ng/mL for perindopril in human plasma. The lower limit of quantification was 0.1 ng/mL with a relative standard deviation of less than 6.1%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 450 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability and bioequivalence studies.

Liquid chromatography tandem mass spectrometry method for the quantification of rimonabant, a CB1 receptor antagonist, in human plasma

“…In recent years, a number of laboratories have reported high-throughput bioanalytical procedures using LC-MS/ MS (Jemal, 2000;Nirogi et al, 2006;Ramakrishna et al, 2006). Our method is simple, sensitive, rapid and specific, which makes it an attractive procedure in highthroughput bioanalysis.…”

Selective spectrofluorimetric method for the determination of perindopril erbumine in bulk and tablets through derivatization with dansyl chloride

“…Literature reported only few analytical methods for the determination of PDE in its bulk, dosage forms and human plasma, such as high performance liquid chromatography (Raju and Rao 2011;Zaazaa et al 2013;Riyaz et al 2012;Chaudhary et al 2010;Jogia et al 2010;Joseph et al 2011;Prajapati et al 2011a), HPLC-MS (Jaina et al 2006 andNirogi et al 2006), high performance thin layer chromatography (Dewani et al 2011), and spectrophotometry (Neelam et al 2012;Rahman et al 2012;Prajapati et al 2011b). However, the chromatographic methods were found to have certain drawbacks, such as the expensive instrumentation and high analysis cost.…”

Novel Spectrofluorimetric Method for the Determination of Perindopril Erbumine Based on Charge Transfer Reaction with 7-Hydroxycoumarin

“…Literature reported only few analytical methods for the determination of PDE in its bulk, dosage forms and human plasma, such as high performance liquid chromatography [2][3][4][5][6][7][8][9], HPLC-MS [10,11], high performance thin layer chromatography [12], and spectrophotometry [13][14][15][16].…”