High-throughput sequencing of hair follicle development-related micrornas in cashmere goat at various fetal periods

Abstract

Inner Mongolia cashmere goat marks a precious gerplasm genetic resource due to its excellent cashmere traits. Therefore, it is of crucial importance to investigate the cashmere development mechanism of cashmere goat and to search for the important cashmere growth-related candidate genes. Fetal skin samples at 10 different periods of cashmere goat were collected in this research. Moreover, high-throughput sequencing was conducted on RNA samples from side skin of cashmere goat fetuses collected at three critical periods of skin hair follicle initiation, growth and development (namely, 45, 55 and 65 days) after balanced mix in line with the previous research results. Meanwhile, 3 samples at corresponding periods were used as the biological duplications. Data regarding microRNA and mRNA expression in skin and hair follicles of cashmere goats at various fetal periods were obtained using the high-throughput sequencing method. The results indicated that microRNAs in the oar-let-7 and oar-miR-200 families in 55 days and 66 days of pregnancy samples had been notably up-regulated relative to those in 45 days of pregnancy samples. This revealed that they might be the critical microRNAs in hair follicle development.

Transcriptomic Analysis Reveals the Involvement of lncRNA–miRNA–mRNA Networks in Hair Follicle Induction in Aohan Fine Wool Sheep Skin

“…miR-143 was detected in our study and has also been reported in association with the HF growth of HU sheep ( Huttenhofer et al, 2005 ). Reports have indicated that the miR-200 family may be a key miRNA in hair follicle development ( Hoefert et al, 2018 ; Liu et al, 2018 ); thus, we speculate that miR-200b, which was identified in our study, may play a role in the growth of HF of AFWS. Our results were consistent with these reports, provide guidance for our research.…”

Effect of Nutritional Restriction on the Hair Follicles Development and Skin Transcriptome of Chinese Merino Sheep

“…The morphogenesis of wool follicles in Merino sheep is similar to those of cashmere goats [ 8 ]. Studies on wool follicle cycling and SF development in cashmere goats have identified a large number of genes related to follicle and fiber development in which the mechanism of SF development has been elucidated using high throughput RNA sequencing approach [ 9 , 10 , 11 , 12 ]. However, the morphogenesis and genes or signaling pathways that regulate fetal SF development and branching in Merino sheep require further investigation.…”

Conservation Assessment of the State Goat Farms by Using SNP Genotyping Data

“…Although all the goat farms have more than six unrelated groups of goats, their effective population size followed a decreasing trend. The structure analysis result revealed that the genetic background of ZWM goats was also present in the ACM breeds ( Figure 3 c) which might be attributed to the presence of gene flow due to close geographic distribution or artificial insemination used to develop the ACM goat breed for cashmere traits [ 26 , 27 ]. The structure result indicated that some of the individuals of the ZWM goat breed did not differentiate completely which could be due to the recent establishment of these individuals or high gene flow in this breeds.…”