2007
DOI: 10.1016/j.jbiotec.2007.06.023
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High-throughput SNP genotyping based on solid-phase PCR on magnetic nanoparticles with dual-color hybridization

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Cited by 24 publications
(17 citation statements)
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“…The reported detectable signals should meet with two criteria: greater than the background value plus three times of background standard deviation and spot co-variance (CV = stdev × 100/intensity) less than 50%. [29][30][31][32] Generation of comparison data sets from detected signals included log 2 -transformation, gene centering and normalization. Hierarchical clustering was performed by average linkage and Euclidean distance metric.…”
Section: Hybridization Data Analysismentioning
confidence: 99%
“…The reported detectable signals should meet with two criteria: greater than the background value plus three times of background standard deviation and spot co-variance (CV = stdev × 100/intensity) less than 50%. [29][30][31][32] Generation of comparison data sets from detected signals included log 2 -transformation, gene centering and normalization. Hierarchical clustering was performed by average linkage and Euclidean distance metric.…”
Section: Hybridization Data Analysismentioning
confidence: 99%
“…However, these platforms mostly based on RFBE or single base extension strategies, which require very expensive enzyme or fluorescent dye labeled probe and increase the cost for large scale genotyping. We have developed a method for SNP genotyping based on MNPs and dualcolor hybridization before, [43][44] in which the genotyping efficiency and accuracy were highly improved using MNPs. However, a different pair of fluorescent probes was still necessary when analysis each SNP locus, so the cost will also be very high when dealing with thousands of DNA samples for multiplex SNP loci.…”
Section: Discussionmentioning
confidence: 99%
“…In recent years, magnetic nanoparticles have attracted a great deal of interest in various fields of advanced biological and medical applications, such as RNA and DNA purification, immunoassays, cell isolation, and DNA detection. [24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42] Based on using magnetic nanoparticles as target DNA carrier, we have developed couple of genotyping methods previously which avoid any complex procedure including purification and concentration, [43][44] however, it still requires two types of labeled probes for each SNP locus, it increase the cost on the reagents when doing multiplexing analysis.…”
Section: Introductionmentioning
confidence: 99%
“…Single nucleotide polymorphisms (SNPs) are point mutations that are the most abundant forms of sequence variations between individuals and occur at a frequency of about one per 500-1000 bp in human genome (Liu et al, 2007;Marshall, 1997). Many pathogenic and genetic diseases such as cystic fibrosis, Alzheimer's, sickle cell anemia and certain cancers are associated with SNPs (Sachidanandam et al, 2001) or changes in the sequence of particular genes (Pang et al, 2007).…”
Section: Introductionmentioning
confidence: 99%
“…For their unique high dispersion capability in aqueous solution, high separation efficiency in magnetic field, magnetic nanoparticles (NPs) have been successfully used in various fields of biology and medicine such as magnetic drug targeting, magnetic resonance imaging, immunoassays, cell separation, RNA and DNA purification (Ingram et al, 2005;Liu et al, 2007;Niemeyer, 2001;Zhao et al, 2003). However, one of the major obstacles is the lack of surface tenability for biocompatible applications.…”
Section: Introductionmentioning
confidence: 99%