High-Virulence Clone of Group B Streptococci Unable to Grow at High Temperatures Is Present in Serotypes Other Than Type III

Palacios, Gerardo C.; González, María Natalia Gómez; Beltrán, Magdalena; Arredondo, José Luis; Torres, Javier; Solórzano, Fortino

doi:10.1007/s00284-006-0291-3

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…Infeksi ginjal ini diduga akibat adanya serangan S. agalactiae yang diinjeksikan ke dalam tubuh ikan uji sehingga mengakibatkan penurunan jumlah eritrosit. Palacios et al (2007) mengatakan bahwa salah satu toksin yang dikeluarkan oleh bakteri S. agalactiae adalah hyaluronidase yang berfungsi sebagai faktor penyebaran. Van Calsteren et al 2010dan Tang et al (2012) juga menyatakan toksin lain yang dihasilkan oleh S. agalactiae adalah superoxide dismutase dan kapsul polisakarida.…”

Section: Parameter Hematologiunclassified

Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

Fauziah¹,

Wahjuningrum²,

Sukenda³

et al. 2015

Jurnal Akuakultur Indonesia

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ABSTRACT This study was aimed at determining potential of combination powder of garlic Allium sativum-shatterstone herb Phyllanthus niruri supplemented in feed against S. agalactiae infection in tilapia. Four concentrations of combination powder of A. sativum-P. Niruri; 20+5, 20+10, 20+15 and 20+20 ppt respectively were investigated for their ability to inhibit bacterial fish pathogen. Combination dose of 20+15 ppt produced the highest inhibitory zones in in vitro test. In vivo test consisted of three treatments with three replications, namely positive control (K+), negative control (K-) and the treatment of A. sativum-P. niruri suplemented in feed (BM). The test perfomed on tilapia with weight of 10.33 ± 1.63 g and were reared at density of 10 ind/aquarium. The fish was fed for 14 days, then injected intraperitoneally with 0.1 mL S. agalactiae at concentration of 105 cfu/mL for positive control and BM groups. Survival, growth rate, feed response, hematological and water quality parameters were observed for 10 days. This study showed that the suplemented-feed-fish (BM) showed better growth rate, feed response, and survival (83.3%) than positive control (36.7%) at P<0.05. In addition, A. sativum-P. niruri suplemented in feed was also able to enhance the immune response by increasing phagocytic activity. Keywords: Streptococcus agalactiae, phytopharmacy, Allium sativum-Phyllanthus niruri, tilapia ABSTRAK Penelitian ini bertujuan untuk menganalisis potensi campuran tepung bawang putih Allium sativum-meniran Phyllanthus niruri dalam pakan terhadap pencegahan infeksi bakteri S. agalactiae pada ikan nila. Empat konsentrasi campuran tepung bawang putih-meniran yaitu 20+5 ppt, 20+10 ppt, 20+15 ppt dan 20+20 ppt masing-masing diuji kemampuannya dalam menghambat bakteri patogen pada ikan. Campuran dosis 20+15 ppt menghasilkan zona hambat terbaik dalam uji in vitro. Uji in vivo terdiri atas tiga perlakuan dengan tiga ulangan yaitu kontrol positif, kontrol negatif, dan perlakuan pakan yang mengandung bawang putih-meniran (BM). Uji ini dilakukan pada ikan nila berbobot 10,33±1,63 g yang dipelihara di akuarium dengan kepadatan 10 ekor/akuarium. Ikan diberi pakan perlakuan selama 14 hari kemudian diinjeksi secara intraperitoneal dengan bakteri S. agalactiae sebanyak 0,1 mL dengan kepadatan 105 cfu/mL pada perlakuan kontrol positif dan perlakuan BM. Parameter kelangsungan hidup, laju pertumbuhan, respons pakan, parameter hematologi, dan kualitas air diamati selama sepuluh hari. Hasil dari penelitian ini menunjukkan bahwa pemberian BM dalam pakan memberikan laju pertumbuhan, respons pakan, dan sintasan (83,3%) yang lebih baik daripada kontrol positif (36,7%) pada P<0,05. Pakan yang mengandung campuran bawang putih-meniran ini juga mampu meningkatkan respons imun dengan adanya peningkatan aktivitas fagositosis. Kata kunci: Streptococcus agalactiae, fitofarmaka, Allium sativum-Phyllanthus niruri, ikan nila

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Section: Parameter Hematologiunclassified

Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

Fauziah¹,

Wahjuningrum²,

Sukenda³

et al. 2015

Jurnal Akuakultur Indonesia

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“…Bacterial incubation at temperatures closer to that occurring in patients with severe infection and high fever (40˚C) induced differences in GBS genes implicated in virulence, as the up-regulation of several transcripts. For example, the genes in the hemolysin operon cyl, CAMP factor (an extracellular cytolytic protein involved in GBS pathogenesis), proteins with LPXTG motifs, chaperones, heat-shock proteins and a heat-inducible transcription regulator were clearly overexpressed at 40˚C (4,5).…”

Section: Introductionmentioning

confidence: 99%

Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells

Lione

Santos²,

Carvalho³

et al. 2010

Int J Mol Med

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Abstract. Group B streptococci (GBS) are the most common cause of pneumonia and sepsis during the neonatal period. However, the pathogenesis of invasive infection is poorly understood. We investigated the ability of GBS grown at 37˚C and 40˚C to adhere and invade human umbilical vein endothelial cells (HUVECs) at different periods of incubation (0, 0.5, 1, 2, 18 and 24 h). All strains tested, except strain 88641-vagina survived for 24 h in the intracellular environment at 40˚C. For serotype III grown at 40˚C, both strains (80340-vagina and 90356-liquor) showed increased adherence and intracellular survival when compared to bacteria grown at 37˚C (P<0.01). GBS serotype V strains (88641-vagina and 90186-blood) showed ability to survive inside HUVECs until 2 and 24 h post-infection at 40˚C and 37˚C, respectively (P<0.01). Influence of growth temperature in bacterial interaction with endothelial cells was partially dependent of serotypes and the clinical origin of strains. Serotypes III and V strains grown at both temperatures remained viable within acidic endothelial vacuoles which acquired Rab7 and LAMP-1 endosomal markers. The data emphasize the influence of temperature on cellular events of phagocytosis and pathogenesis of GBS diseases.

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High-Virulence Clone of Group B Streptococci Unable to Grow at High Temperatures Is Present in Serotypes Other Than Type III

Cited by 2 publications

References 23 publications

Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

Combination of garlic - shatterstone herb powder to control Streptococcus agalactiae infection in tilapia

Fever temperature enhances mechanisms of survival of Streptococcus agalactiae within human endothelial cells

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