High-Yield Activation of Scaffold Polymer Surfaces To Attach Cell Adhesion Molecules

Dennes, T. Joseph; Hunt, Geoffrey C.; Schwarzbauer, Jean E.; Schwartz, Jeffrey

doi:10.1021/ja065217t

Cited by 26 publications

(37 citation statements)

References 35 publications

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“…Because the organometallic-based interface can be formed on essentially any oxide-terminated surface or on organic polymers that contain appropriate ligating atoms, our patterned interface method should be readily translatable to a broad spectrum of hard and soft biomaterial surfaces. 28, 29, 32-34, 44, 45 This work represents to the best of our knowledge the first example of creating a patterned nanoscale, abiologic interface, as synthesized, to control cell adhesion and spreading on flexible polymer surfaces of biomedical relevance. Because our method does not rely on the surface attachment of any specific cell surface receptor ligands, the SAMP/ZrO 2 interface should be permissive for adhesion of a variety of cell types.…”

Section: Discussionmentioning

confidence: 99%

A simple nanoscale interface directs alignment of a confluent cell layer on oxide and polymer surfaces

et al. 2013

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Templating of cell spreading and proliferation is described that yields confluent layers of cells aligned across an entire two-dimensional surface. The template is a reactive, two-component interface that is synthesized in three steps in nanometer thick, micron-scaled patterns on silicon and on several biomaterial polymers. In this method, a volatile zirconium alkoxide complex is first deposited at reduced pressure onto a surface pattern that is prepared by photolithography; the substrate is then heated to thermolyze the organic ligands to form surface-bound zirconium oxide patterns. The thickness of this oxide layer ranges from 10 to 70 nanometers, which is controlled by alkoxide complex deposition time. The oxide layer is treated with 1,4-butanediphosphonic acid to give a monolayer pattern whose composition and spatial conformity to the photolithographic mask are determined spectroscopically. NIH 3T3 fibroblasts and human bone marrow-derived mesenchymal stem cells attach and spread in alignment with the pattern without constraint by physical means or by arrays of cytophilic and cytophobic molecules. Cell alignment with the pattern is maintained as cells grow to form a confluent monolayer across the entire substrate surface.

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Section: Discussionmentioning

confidence: 99%

A simple nanoscale interface directs alignment of a confluent cell layer on oxide and polymer surfaces

et al. 2013

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“…Dennes and colleagues showed how acidic surfaces can be activated by zirconium tetra( tert -butoxide) for binding of RGD peptides using a maleimide cross linker 13, 15 . Our study advances the studies by Dennes and co-workers by demonstrating a facile technique for conjugating larger protein domains to zirconium activated polyurethane surfaces using a nitrophenyl carbonate cross linker.…”

Section: Discussionmentioning

confidence: 99%

“…After the last deposition step, the samples were heated to 45 °C and held at this temperature for five minutes to convert the zirconium alkoxide layer to a cross-linked mixed alkoxide-oxide. Samples were then cooled and further converted to the cross-linked oxide chemical adhesion layer ( 2 ) by heating in a microwave oven for 30 seconds under high power (Figure 1C) 15 . The samples were then suspended vertically in a 0.1 mM solution of 11-phosphonoundecanol ( 3 ) in ethanol (Figure 1C), and the solution was allowed to evaporate.…”

Section: Methodsmentioning

confidence: 99%

Surface Derivatization Strategy for Combinatorial Analysis of Cell Response to Mixtures of Protein Domains

Chiang¹,

Karuri

Kshatriya³

et al. 2011

Langmuir

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We report a robust strategy for conjugating mixtures of two or more protein domains to non-fouling polyurethane surfaces. In our strategy, the carbamate groups of polyurethane are reacted with zirconium alkoxide from the vapor phase to give a surface bound oxide that serves as a chemical layer that can be used to bond organics to the polymer substrate. An hydroxyalkylphosphonate monolayer was synthesized on this layer, which was then used to covalently bind primary amine groups in protein domains using chloroformate-derived cross-linking. The effectiveness of this synthesis strategy was gauged by using an ELISA to measure competitive, covalent bonding of cell-binding (III9–10) and fibronectin-binding (III1–2) domains of the cell adhesion protein fibronectin. Cell adhesion, spreading, and fibronectin matrix assembly were examined on surfaces conjugated with single domains, a 1:1 surface mixture of III1–2 and III9–10, and a recombinant protein “duplex” containing both domains in one fusion protein. The mixture performed as well or better than the other surfaces in these assays. Our surface activation strategy is amenable to a wide range of polymer substrates and free amino group-containing protein fragments. As such, this technique may be used to create biologically specific materials through the immobilization of specific protein groups or mixtures thereof on a substrate surface.

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“…However, one should also consider that only about 40% e.g. [16] of the available monomers in a given polymer strand are accessible to the surface (the remainder penetrate into the bulk e.g. [17]).…”

Section: Some Examples To Test the Equationsmentioning

confidence: 99%

Semi-empirical description of the constant β in the equation of state for interfacial tension

Skrdla¹

2011

Journal of Colloid and Interface Science

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High-Yield Activation of Scaffold Polymer Surfaces To Attach Cell Adhesion Molecules

Cited by 26 publications

References 35 publications

A simple nanoscale interface directs alignment of a confluent cell layer on oxide and polymer surfaces

A simple nanoscale interface directs alignment of a confluent cell layer on oxide and polymer surfaces

Surface Derivatization Strategy for Combinatorial Analysis of Cell Response to Mixtures of Protein Domains

Semi-empirical description of the constant β in the equation of state for interfacial tension

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