High yield fabrication of multilayer polydimethylsiloxane devices with freestanding micropillar arrays

Gregory, Christopher; Sellgren, Katelyn L.; Gilchrist, Kristin H.; Grego, Sonia

doi:10.1063/1.4827600

Cited by 10 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fabrication details of membrane-integrated devices are described elsewhere. 15,16 In order to achieve a physiologically relevant fluid shear stress of 5 dyn/cm 2 (Refs. 11 and 17) on a 150 lm tall flow channel, a fluid flow of 120 ll/min was used.…”

Section: Methodsmentioning

confidence: 99%

An optically transparent membrane supports shear stress studies in a three-dimensional microfluidic neurovascular unit model

2015

Self Cite

View full text Add to dashboard Cite

We report a microfluidic blood-brain barrier model that enables both physiological shear stress and optical transparency throughout the device. Brain endothelial cells grown in an optically transparent membrane-integrated microfluidic device were able to withstand physiological fluid shear stress using a hydrophilized polytetrafluoroethylene nanoporous membrane instead of the more commonly used polyester membrane. A functional three-dimensional microfluidic co-culture model of the neurovascular unit is presented that incorporates astrocytes in a 3D hydrogel and enables physiological shear stress on the membrane-supported endothelial cell layer.

show abstract

Section: Methodsmentioning

confidence: 99%

An optically transparent membrane supports shear stress studies in a three-dimensional microfluidic neurovascular unit model

2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…Challenges, however, in long-term reliability and maintenance of high-quality recordings have prevented the widespread clinical adoption of overfilling of prepolymer prevent the formation of independent device features. Current solutions such as cure inhibition [21] or physical expulsion of the excess prepolymer [22] and etching of the membrane [23] result in rough or sticky surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…These membranes caused by the overfilling of prepolymer prevent the formation of independent device features. Current solutions such as cure inhibition [ 21 ] or physical expulsion of the excess prepolymer [ 22 ] and etching of the membrane [ 23 ] result in rough or sticky surfaces.…”

Section: Introductionmentioning

confidence: 99%

Mechanically Matched Silicone Brain Implants Reduce Brain Foreign Body Response

Zhang

Clément

Alameri

et al. 2021

Adv Materials Technologies

View full text Add to dashboard Cite

Brain implants are increasingly used to treat neurological disorders and diseases. However, the brain foreign body response (FBR) elicited by implants affects neuroelectrical transduction and long‐term reliability limiting their clinical adoption. The mismatch in Young's modulus between silicon implants (≈180 GPa) and brain tissue (≈1–30 kPa) exacerbates the FBR, resulting in the development of flexible implants from polymers such as polyimide (≈1.5–2.5 GPa). However, a stiffness mismatch of at least two orders of magnitude remains. The study introduces 1) the first mechanically matched brain implant (MMBI) made from silicone (≈20 kPa); 2) new microfabrication methods; and 3) a novel dissolvable sugar shuttle to reliably implant MMBIs. MMBIs are fabricated via vacuum‐assisted molding using sacrificial sugar molds and are then encased in sugar shuttles that dissolved within 2 min after insertion into rat brains. Sections of rat neocortex implanted with MMBIs, polydimethylsiloxane (PDMS) implants, and silicon implants are analyzed by immunohistochemistry 3 and 9 weeks post‐implantation. MMBIs result in significantly higher neuronal density and lower FBR within 50 µm of the tissue‐implant interface compared to PDMS and silicon implants, suggesting that materials mechanically matched to brain further minimize the FBR and can contribute to better implant functionality and long‐term reliability.

show abstract

“…These membranes caused by the overfilling of prepolymer prevent the formation of independent device features. Current solutions such as cure inhibition [21] or physical expulsion of the excess prepolymer [22] and etching of the membrane [23] results in rough or sticky surfaces.…”

Section: Introductionmentioning

confidence: 99%

Mechanically Matched Silicone Brain Implants Reduce Brain Foreign Body Response

Zhang

Alameri

Clément

et al. 2020

Preprint

View full text Add to dashboard Cite

Brain implants are increasingly used to treat neurological disorders and diseases. However, the brain foreign body response (FBR) elicited by implants affects neuro-electrical transduction and long-term reliability limiting their clinical adoption. The mismatch in Young’s modulus between silicon implants (∼180 GPa) and brain tissue (∼1-30 kPa) exacerbates the FBR resulting in the development of flexible implants from polymers such as polyimide (∼1.5-2.5 GPa). However, a stiffness mismatch of at least two orders of magnitude remains. Here, we introduce (i) the first mechanically matched brain implant (MMBI) made from silicone (∼20 kPa), (ii) new microfabrication methods, and (iii) a novel dissolvable sugar shuttle to reliably implant MMBIs. MMBIs were fabricated via vacuum-assisted molding using sacrificial sugar molds and were then encased in sugar shuttles that dissolved within 2 min after insertion into rat brains. Sections of rat neocortex implanted with MMBIs, PDMS implants, and silicon implants were analyzed by immunohistochemistry 3 and 9-weeks post-implantation. MMBIs resulted in significantly higher neuronal density and lower FBR within 50 µm of the tissue-implant interface compared to PDMS and silicon implants suggesting that materials mechanically matched to brain further minimize the FBR and could contribute to better implant functionality and long-term reliability.

show abstract

High yield fabrication of multilayer polydimethylsiloxane devices with freestanding micropillar arrays

Cited by 10 publications

References 19 publications

An optically transparent membrane supports shear stress studies in a three-dimensional microfluidic neurovascular unit model

An optically transparent membrane supports shear stress studies in a three-dimensional microfluidic neurovascular unit model

Mechanically Matched Silicone Brain Implants Reduce Brain Foreign Body Response

Mechanically Matched Silicone Brain Implants Reduce Brain Foreign Body Response

Contact Info

Product

Resources

About