High yield ultrasonication extraction method for Undaria pinnatifida sporophyll and its anti-inflammatory properties associated with AP-1 pathway suppression

Song, Kyung‐Mo; Ha, Su Jong; Lee, Jang-Eun; Kim, Soonhee; Kim, Yong Ho; Kim, Young-Eon; Hong, Sang Pil; Jung, Sung Keun; Lee, Nam Hyouck

doi:10.1016/j.lwt.2015.07.055

Cited by 41 publications

(24 citation statements)

References 29 publications

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“…Improved yield achieved by UAE in comparison to CE is attributed to the bubble cavitation phenomenon generated by ultrasonic waves [75], that was previously observed in fucoidan extraction from Sargassum witghtii [70], Undaria pinnatifida [76] and laminarin extraction from A. nodosum and Laminaria hyperborean [75]. No statistical difference between UAE and CE was reported in fucoidan extraction from N. zanardinii [64] and Fucus evanescens [77], while Okolie et al [65] reported significantly higher yield by CE (11.9%) than by UAE (4.56%) and also no statistical differences between UAE, MAE and EAE.…”

Section: Ultrasound Assisted Extraction (Uae)mentioning

confidence: 61%

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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Over the years, brown algae bioactive polysaccharides laminarin, alginate and fucoidan have been isolated and used in functional foods, cosmeceutical and pharmaceutical industries. The extraction process of these polysaccharides includes several complex and time-consuming steps and the correct adjustment of extraction parameters (e.g., time, temperature, power, pressure, solvent and sample to solvent ratio) greatly influences the yield, physical, chemical and biochemical properties as well as their biological activities. This review includes the most recent conventional procedures for brown algae polysaccharides extraction along with advanced extraction techniques (microwave-assisted extraction, ultrasound assisted extraction, pressurized liquid extraction and enzymes assisted extraction) which can effectively improve extraction process. The influence of these extraction techniques and their individual parameters on yield, chemical structure and biological activities from the most current literature is discussed, along with their potential for commercial applications as bioactive compounds and drug delivery systems.(APS) is determined by algae species however it is also influenced by other factors causing inter-species variation, e.g., growth location and harvesting season [8]. Vast structural variation between the APS therefore presents a challenge in terms of pre-treatments application, extraction techniques and optimization, characterization of isolated fractions and determination of their biological properties.Chemical structure and yield of APS isolated from marine macroalgae by conventional extraction (CE) techniques can be affected by various experimental conditions (pH, time, temperature, pressure, particle size, solvent, sample to solvent ratio, agitation speed etc.). In addition, different advanced techniques such as microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), pressurized liquid extraction (PLE), enzyme-assisted extractions (EAE) are assessed and applied for APS extraction [9][10][11].In general, the chemical structure of polysaccharides determines its physical, chemical and biochemical properties as well as its biological activities [12]. Several studies have reported that their biological activity is strongly associated with their chemical structure [9]. Due to very complex mechanisms that are affected by many factors, the correlation between polysaccharide structure and biological activity is still not sufficiently clarified.In order to improve isolation of APS, pre-treatments are usually applied to the algal biomass prior to the extraction process with the two aims: (i) to prevent co-extraction of interfering bioactive compounds with similar solubility; and (ii) to disrupt cell walls and improve mass transfer of APS into extraction solvent. The first type of pre-treatments is therefore used to remove compounds which are highly bound to the APS such as proteins, phenols and lipids, as well as mannitol and chlorophyll [13]. For that purpose, the application of various pr...

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Section: Ultrasound Assisted Extraction (Uae)mentioning

confidence: 61%

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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“…In fact, NF-κB plays a critical role in LPS-mediated iNOS expression and NO production. In a previous study, we found that seaweed extracts affect the phosphorylation of MAPKs, but not NF-κB signaling pathways by using SR2001, a specific AP-1 inhibitor [48]. We demonstrated that only AP-1 can regulate LPS-induced iNOS expression and NO production in RAW 264.7 cells.…”

Section: Discussionmentioning

confidence: 64%

Green Pepper (Piper nigrum L.) Extract Suppresses Oxidative Stress and LPS-Induced Inflammation via Regulation of JNK Signaling Pathways

Kim

Shin

et al. 2020

Applied Sciences

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In this study, we compared the physicochemical properties and the antioxidant capacities of black and green pepper. Green pepper from India (GPI) and Sri Lanka (GPS) had higher Hunter L* and b* values and lower a* values than black pepper from India (BPI) and Sri Lanka (BPS). The contents of chlorophyll a and b, flavonoids, and phenolic compounds in GPI and GPS were higher than those in BPI and BPS. The peppercorns showed the following decreasing order of 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging capacities: GPI > GPS > BPI > BPS. High-performance liquid chromatography showed that the highest piperine content was present in GPI (8613.27 ± 45.86 mg/100 g). We further investigated the anti-inflammatory capacity of the green pepper. GPS and GPI significantly suppressed lipopolysaccharide (LPS)-induced nitrite production and inducible nitric oxide synthase expression without being cytotoxic to RAW 264.7 cells. GPS and GPI also suppressed the LPS-induced phosphorylation of mitogen-activated protein kinases, but not p65. GPS had a higher inhibitory effect on LPS-induced c-Jun phosphorylation and translocation from the cytosol to the nucleus than GPI. Thus, the findings of our study suggest that green pepper has the potential to be an effective nutraceutical against oxidative and inflammatory stress.

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“…Under the optimal conditions of UAE, the TPS content increased by 16.8% compared to that of HWE. According to previous study carried out on Undaria pinnatifida (U. pinnatifida) to extract fucoidan, while yield of fucoidan extracted by HWE method was 12.9%, that extracted by UAE was 33.0% . The higher extraction yield can be explained because the formation of cavitation by ultrasonic wave degrades brown seaweed cell wall, leading to the acceleration of the extraction of fucoidan …”

Section: Resultsmentioning

confidence: 97%

Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum mcclurei and Testing Bioactivities of the Extract

Sung

Dat

et al. 2020

ChemistrySelect

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In this study, fucoidan was extracted from the Vietnamese brown seaweed Sargassum mcclurei by ultrasound -assisted extraction method (UAE) using ethanol as a solvent. The effects of single factors on total polysaccharide (TPS) content were then investigated, followed by the screening of main factors affecting TPS content based on their effect sizes and p-values in analysis of variance. The response surface methodology using the Box-Behnken design was applied to investigate simultaneous effects of four main factors including solvent-tomaterial ratio, extraction time, ultrasonic power, and extraction temperature. The optimal conditions for fucoidan extraction were identified with solvent-to-material ratio of 24 : 1 mL/g, extraction time of 49 min, ultrasonic power of 360 W, and extraction temperature of 54 � C. The highest yield of TPS obtained under these conditions was 7.1203 mg/g. Besides, extraction of fucoidan using hot water method was also carried out to compare with UAE method in terms of TPS content. The bioactivity tests showed the extract had the anti-oxidation activity with the half-maximal inhibitory concentration (IC 50 ) value of 0.4261 � 0.0008 mg/mL, cytotoxic effect on breast carcinoma cells and human lung carcinoma with IC 50 values of 95.80 � 4.75 and 129.00 � 0.66 μg/mL, respectively.

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High yield ultrasonication extraction method for Undaria pinnatifida sporophyll and its anti-inflammatory properties associated with AP-1 pathway suppression

Cited by 41 publications

References 29 publications

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Green Pepper (Piper nigrum L.) Extract Suppresses Oxidative Stress and LPS-Induced Inflammation via Regulation of JNK Signaling Pathways

Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum mcclurei and Testing Bioactivities of the Extract

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