Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed <i>Sargassum mcclurei</i> and Testing Bioactivities of the Extract

My, Phan Le Thao; Sung, Vo Van; Dat, Tran Do; Nam, Hoàng Minh; Phong, Mai Thanh; Hieu, Nguyen Huu

doi:10.1002/slct.201903818

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…The authors reported the highest extraction yield of fucoidan from conventional chemical (11.9%) and microwave-assisted (5.71%) extraction methods. In another study, fucoidan was extracted from Sargassum mcclurei brown seaweed by an ultrasound-assisted extraction method using ethanol and reported a yield of 7.1 mg/g of total polysaccharide content (representing fucoidan) in the extract [29]. The results are expressed as mean ± standard deviation of the mean (n = 2).…”

Section: Biochemical Composition Analysismentioning

confidence: 99%

Purification and Molecular Characterization of Fucoidan Isolated from Ascophyllum nodosum Brown Seaweed Grown in Ireland

et al. 2023

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The present study investigates the molecular characteristics of fucoidan obtained from the brown Irish seaweed Ascophyllum nodosum, employing hydrothermal-assisted extraction (HAE) followed by a three-step purification protocol. The dried seaweed biomass contained 100.9 mg/g of fucoidan, whereas optimised HAE conditions (solvent, 0.1N HCl; time, 62 min; temperature, 120 °C; and solid to liquid ratio, 1:30 (w/v)) yielded 417.6 mg/g of fucoidan in the crude extract. A three-step purification of the crude extract, involving solvents (ethanol, water, and calcium chloride), molecular weight cut-off filter (MWCO; 10 kDa), and solid-phase extraction (SPE), resulted in 517.1 mg/g, 562.3 mg/g, and 633.2 mg/g of fucoidan (p < 0.05), respectively. In vitro antioxidant activity, as determined by 1,1-diphenyl-2-picryl-hydrazyl radical scavenging and ferric reducing antioxidant power assays, revealed that the crude extract exhibited the highest antioxidant activity compared to the purified fractions, commercial fucoidan, and ascorbic acid standard (p < 0.05). The molecular attributes of biologically active fucoidan-rich MWCO fraction was characterised by quadruple time of flight mass spectrometry and Fourier-transform infrared (FTIR) spectroscopy. The electrospray ionisation mass spectra of purified fucoidan revealed quadruply ([M+4H]4+) and triply ([M+3H]3+) charged fucoidan moieties at m/z 1376 and m/z 1824, respectively, and confirmed the molecular mass 5444 Da (~5.4 kDa) from multiply charged species. The FTIR analysis of both purified fucoidan and commercial fucoidan standard exhibited O-H, C-H, and S=O stretching which are represented by bands at 3400 cm−1, 2920 cm−1, and 1220–1230 cm−1, respectively. In conclusion, the fucoidan recovered from HAE followed by a three-step purification process was highly purified; however, purification reduced the antioxidant activity compared to the crude extract.

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Section: Biochemical Composition Analysismentioning

confidence: 99%

Purification and Molecular Characterization of Fucoidan Isolated from Ascophyllum nodosum Brown Seaweed Grown in Ireland

et al. 2023

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“…55 When alcohol was used as a solvent in ultrasonic extraction, the fucoidan yield increased by 16.8%, compared to the extraction with hot water. In this way, fucoidan was obtained from Sargassum mcclurei at a solvent to algae ratio of 24:1, the extraction time of 49 min at 54°C, with the ultrasound power being 360 W. 56 Ultrasonic exposure is also known to lead to an increase in enzyme activity when used together. The enzymatic ultrasonic extraction method allows for obtaining a lower molecular weight polysaccharide with a higher fucoidan yield compared to the ultrasonic method (from 3.6% to 7.87%).…”

Section: Ultrasonic Extraction Of Fucoidanmentioning

confidence: 99%

Ultrasonic-treated fucoidan as a promising therapeutic agent

Suprunchuk¹

2021

Polim. Med.

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“…In UCGE, ultrasonic energy can destroy the plant cell membrane, accelerate the molecular movement, and improve the mass transfer rate. Thus, the UCGE has been gradually applied to traditional Chinese medicine and functional foods [19–21] …”

Section: Introductionmentioning

confidence: 99%

Ultrasonic Cell Grinder Extraction of Cyperenoic Acid from Crotonis Crassifolii Radix and the Activity Assay of the Extract

Jiang

et al. 2022

ChemistrySelect

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It is of great significance to achieve a high‐value usage of Chinese medicine residues. This study developed a green ultrasonic cell grinder extraction (UCGE) method has been developed for cyperenoic acid extraction from water decocted Crotonis Crassifolii Radix. The response surface methodology was introduced for the experimental design. The optimum conditions were as follows: 52 % ethanol concentration, 28 : 1 mL/g ratio of solution‐to‐solid, 500 W extraction power, and 28 min extraction time. Anti‐inflammatory test was performed to investigate the medicinal uses of the extract from the herb residue. The microstructure of the extracted samples was also investigated using scanning electron microscopy to understand the extraction mechanism. A higher extraction yield of 1.016 mg/g and better anti‐inflammatory activity were obtained under UCGE compared to a conventional solvent extraction. Meanwhile, the sample structure was seriously damaged by UCGE. The results show that UCGE is a promising green and efficient technology for extracting functional components, and has great potential in recycling waste resources.

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Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum mcclurei and Testing Bioactivities of the Extract

Cited by 12 publications

References 34 publications

Purification and Molecular Characterization of Fucoidan Isolated from Ascophyllum nodosum Brown Seaweed Grown in Ireland

Purification and Molecular Characterization of Fucoidan Isolated from Ascophyllum nodosum Brown Seaweed Grown in Ireland

Ultrasonic-treated fucoidan as a promising therapeutic agent

Ultrasonic Cell Grinder Extraction of Cyperenoic Acid from Crotonis Crassifolii Radix and the Activity Assay of the Extract

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