Higher order Arabidopsis 14-3-3 mutants show 14-3-3 involvement in primary root growth both under control and abiotic stress conditions

Kleeff, Paula J. M. van; Jaspert, Nina; Li, Ka Wan; Rauch, Sabine; Oecking, Claudia; Boer, Albertus H. de

doi:10.1093/jxb/eru338

Cited by 43 publications

(59 citation statements)

References 55 publications

(89 reference statements)

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“…This is in striking contrast to the mild phenotypes reported even for quadruple knockout mutants of 14-3-3 non-epsilon isoforms (van Kleeff et al, 2014) and might highlight a particular importance of the ancestral epsilon group.…”

Section: Resultscontrasting

confidence: 85%

Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development

Keicher

Jaspert

Weckermann

et al. 2017

eLife

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Eukaryotic 14-3-3 proteins have been implicated in the regulation of diverse biological processes by phosphorylation-dependent protein-protein interactions. The Arabidopsis genome encodes two groups of 14-3-3s, one of which – epsilon – is thought to fulfill conserved cellular functions. Here, we assessed the in vivo role of the ancestral 14-3-3 epsilon group members. Their simultaneous and conditional repression by RNA interference and artificial microRNA in seedlings led to altered distribution patterns of the phytohormone auxin and associated auxin transport-related phenotypes, such as agravitropic growth. Moreover, 14-3-3 epsilon members were required for pronounced polar distribution of PIN-FORMED auxin efflux carriers within the plasma membrane. Defects in defined post-Golgi trafficking processes proved causal for this phenotype and might be due to lack of direct 14-3-3 interactions with factors crucial for membrane trafficking. Taken together, our data demonstrate a fundamental role for the ancient 14-3-3 epsilon group members in regulating PIN polarity and plant development.DOI: http://dx.doi.org/10.7554/eLife.24336.001

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Section: Resultscontrasting

confidence: 85%

Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development

Keicher

Jaspert

Weckermann

et al. 2017

eLife

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“…If the A/N‐invertases bind 14‐3‐3 proteins in vivo , the absence of certain 14‐3‐3 isoforms should inhibit invertase activity and reduce fructose and glucose concentrations in the root. Therefore, we phenotyped three quadruple 14‐3‐3 mutants that we had generated based on six related genes in the non‐epsilon 14‐3‐3 group, namely kappa/lambda/upsilon/nu ( klun ), kappa/lambda/phi/chi ( klpc ) and upsilon/nu/phi/chi ( unpc ) (Van Kleeff et al ., ) by measuring the sugar content of root extracts (Figure a). Intriguingly, only the klpc mutant combination showed strongly reduced levels of the A/N‐invertase products fructose and glucose compared to wild‐type plants (Wt).…”

Section: Resultsmentioning

confidence: 99%

“…Analysis of the function of 14‐3‐3 proteins through mutant phenotyping is a challenging task due to functional redundancy. Nevertheless, the analysis of the sugar levels of the three quadruple mutants that we generated by combining T‐DNA insertions in gene pairs of the non‐epsilon group ( KAPPA/LAMBDA, PHI/CHI and UPSILON/NU ) (Van Kleeff et al ., ) was highly informative, because only one of the three quadruple mutants, klpc , had strongly reduced levels of glucose and fructose which correlated with the lower invertase activity in the root extract of this mutant. Intriguingly, combining kl with un (= klun mutant), or pc with un (= unpc mutant) had no effect on root sugar composition, so apparently KL and PC are redundant for activation of the A/N‐invertase(s).…”

Section: Discussionmentioning

confidence: 99%

Light modulated activity of root alkaline/neutral invertase involves the interaction with 14‐3‐3 proteins

et al. 2014

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Summary Alkaline/neutral invertases (A/N‐Invs) are now recognized as essential proteins in plant life. They catalyze the irreversible breakdown of sucrose into glucose and fructose and thus supply the cells with energy as well as signaling molecules. In this study we report on a mechanism that affects the activity of the cytosolic invertase AtCINV1 (At‐A/N‐InvG or AT1G35580). We demonstrate that Ser547 at the extreme C‐terminus of the AtCINV1 protein is a substrate of calcium‐dependent kinases (CPK3 and 21) and that phosphorylation creates a high‐affinity binding site for 14‐3‐3 proteins. The invertase as such has basal activity, but we provide evidence that interaction with 14‐3‐3 proteins enhances its activity. The analysis of three quadruple 14‐3‐3 mutants generated from six T‐DNA insertion mutants of the non‐epsilon family shows both specificity as well as redundancy for this function of 14‐3‐3 proteins. The strong reduction in hexose levels in the roots of one 14‐3‐3 quadruple mutant plant is in line with the activating function of 14‐3‐3 proteins. The physiological relevance of this mechanism that affects A/N‐invertase activity is underscored by the light‐induced activation and is another example of the central role of 14‐3‐3 proteins in mediating dark/light signaling. The nature of the light‐induced signal that travels from the shoot to root and the question whether this signal is transmitted via cytosolic Ca++ changes that activate calcium‐dependent kinases, await further study.

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“…In particular, 14-3-3-like protein A (gi|357118054, L54, and R136) was identified from both organs. The 14-3-3 proteins function in protein phosphorylation cascades by associating with specific phospho-sites39. They can alter enzyme activity, prevent or induce protein degradation, or affect the subcellular localization of proteins by binding to their targets404142.…”

Section: Discussionmentioning

confidence: 99%

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.Plants encounter a variety of biotic and abiotic stresses during growth 1 . These stresses unbalance cellular homeostasis and lead to morphological, physiological, and molecular changes 2 . These changes have a negative impact on survival and biomass production, and can reduce final yield by up to 82% 3 . Global warming and climate change may also be exacerbating the effects of abiotic stresses on crop production in many areas of the world today. Previous reports have suggested that a temperature increase of 1 °C can produce a decrease in yield of up to 10% 4 . Drought in particular, severely impairs plant growth and development and limits crop productivity more than any other environmental factor 5,6 . As the climate continues to change, drought may become a more frequent and cause severe problem 7 . Drought stress induces a range of physiological and biochemical responses in plants. Under drought conditions, the plant root cap produces abscisic acid (ABA), which mediates stomatal closure. This in turn, suppresses cell growth, photosynthetic efficiency, and respiration [8][9][10] . Recently, ABA and stomata have been implicated in the regulation of systemic responses to abiotic stresses 11 . In addition, plants generate toxic ions and reactive oxygen species (ROS) that can decrease enzyme activities and damage essential proteins 12 . The generation of ROS occurs early in the response to water-stress, and ROS are key secondary messengers triggering subsequent defensive measures in plants 1,[13][14][15] . Plants have developed a sophisticated and elaborate system for scavenging high levels of ROS using antioxidant enzymes that include superoxid...

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Higher order Arabidopsis 14-3-3 mutants show 14-3-3 involvement in primary root growth both under control and abiotic stress conditions

Abstract: SummaryOur research shows that there is isoform specificity and redundancy among 6 out of 13 14-3-3 members in root growth under control and abiotic stress conditions.

Cited by 43 publications

References 55 publications

Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development

Arabidopsis 14-3-3 epsilon members contribute to polarity of PIN auxin carrier and auxin transport-related development

Light modulated activity of root alkaline/neutral invertase involves the interaction with 14‐3‐3 proteins

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

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