Higher prevalence of colonization with Gardnerella vaginalis and gram-negative anaerobes in patients with recurrent miscarriage and elevated peripheral natural killer cells

Kuon, Ruben; Togawa, Riku; Vomstein, Kilian; Weber, Maja; Goeggl, Tamara; Strowitzki, T.; Markert, Udo R.; Zimmermann, Sabine; Daniel, Volker; Dalpke, Alexander H.; Tóth, Bettina

doi:10.1016/j.jri.2017.03.001

Cited by 34 publications

(39 citation statements)

References 34 publications

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“…In the case group, three bacterial taxa were significantly more abundant ( Atopobium, Prevotella and Streptococcus ), while only two taxa were overrepresented in the control group ( Lactobacillus and Gardnerella ). Kuon et al (33) reported that RM patients with elevated peripheral and uterine natural killer cells suffer more frequently from colonization by Gardnerella vaginalis and gram-negative anaerobes. McDonald et al (34) demonstrated that group B streptococcus is a key pathogen in intrauterine infection and a frequent cause of spontaneous midgestation abortions.…”

Section: Discussionmentioning

confidence: 99%

Alteration of vaginal microbiota in patients with unexplained recurrent miscarriage

Zhang

et al. 2019

Exp Ther Med

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The way in which a balanced vaginal microbiome helps prevent gynecological diseases in women and maintain health remains to be fully elucidated. In the present study, the potential effect of aberrations in the vaginal flora on unexplained recurrent miscarriage (RM) was investigated. The vaginal bacterial communities of 10 patients with unexplained RM and 10 healthy volunteers were sampled and subjected to sequencing analysis of the V3-V4 regions of the bacterial 16S ribosomal RNA gene using the Illumina MiSeq platform. Beta diversity analysis/principal component analysis indicated that bacterial community structures were different between the RM and control groups. A lower microbiota diversity in samples from RM patients was revealed by alpha diversity estimation. Taxonomic analysis demonstrated that abundance of three types of phyla ( Firmicutes, Actinobacteria and Bacteroidetes ) was significantly different between the RM and the normal control group. Furthermore, at the genus level, Lactobacillus was the most dominant genus in the two groups. Statistically significant differences were observed in 5 genera between the two groups. In the RM group, 3 bacterial taxa ( Atopobium, Prevotella and Streptococcus ) were significantly more abundant, while only 2 taxa were overrepresented in the control group ( Lactobacillus and Gardnerella ). In conclusion, the present results provide experimental evidence supporting dysbiosis of the vaginal flora in women with RM.

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Section: Discussionmentioning

confidence: 99%

Alteration of vaginal microbiota in patients with unexplained recurrent miscarriage

Zhang

et al. 2019

Exp Ther Med

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“…In recent years, several studies demonstrated that the presence or high load of vaginal microbes associated to BV such as G. vaginalis , A. vaginae , Mobiluncus spp., Mycoplasma spp. and Leptotrichia/Sneathia species, were related with an increased risk of preterm birth ( Foxman et al, 2014 ; Nelson et al, 2014 ; Bretelle et al, 2015 ; Kuon et al, 2017 ). However, the great diversity of vaginal microbiota in women with BV affects its potential to develop preterm birth and other negative outcomes in pregnancy ( Hyman et al, 2014 ; Nelson et al, 2015 ) and it becomes BV treatment in pregnant women a challenging task ( Haahr et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Machado

Castro

Martinez-de-Oliveira

et al. 2017

PeerJ

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BackgroundWe aimed to determine the prevalence of vaginal colonization by Gardnerella vaginalis and of bacterial vaginosis (BV) in Portuguese pregnant women, and to identify risk factors for BV and G. vaginalis colonization in pregnancy.MethodsA cross-sectional study was conducted among pregnant women aged ≥ 18 years who were attending in two public hospitals of the Northwest region of Portugal. Epidemiological data was collected by anonymous questionnaire. BV was diagnosed by Nugent criteria and G. vaginalis presence was identified by polymerase chain reaction. Crude associations between the study variables and BV or G. vaginalis colonization were quantified by odds ratios (ORs) and their 95% confidence intervals (CIs).ResultsThe prevalences of BV and of G. vaginalis colonization among Portuguese pregnant women were 3.88% and 67.48%, respectively. Previous preterm delivery and colonization by G. vaginalis were factors with very high OR, but only statistically significant for a 90% CI. Conversely, higher rates of G. vaginalis colonization were found in women with basic educational level (OR = 2.77, 95% CI [1.33–5.78]), during the second trimester of pregnancy (OR = 6.12, 95% CI [1.80–20.85]) and with BV flora (OR = 8.73, 95% CI [0.50–153.60]).DiscussionDespite the lower number of women with BV, prevalence ratios and association with risk factors were similar to recent European studies. However, the percentage of healthy women colonized by G. vaginalis was significantly higher than many previous studies, confirming that G. vaginalis colonization does not always lead to BV development.

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“…Kuon et al. indicated that patients with RSA had more colonization by Gardnerella vaginalis and Gram-negative anaerobes including Prevotella , Bacteroides , and Veillonella species, based on culture-dependent methods ( Kuon et al., 2017 ). We speculate that the reason for the inconsistent conclusions of these two studies is because of differences in sample size and research methods.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In the past, based on traditional serology and culture methods, Mycoplasma hominis, Ureaplasma urealyticum, Listeria monocytogenes, Gardnerella vaginalis, and other less frequent pathogens were identified more often in patients experiencing spontaneous abortion (Penta et al, 2003;Kuon et al, 2017). However, Contini et al proposed that Mycoplasma hominis and Ureaplasma urealyticum may not cause embryo loss after analyzing bacterial DNA in the aborted tissues of women with early pregnancy loss and women underwent voluntary interruption of pregnancy via quantitative real-time PCR methods (Contini et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Vaginal Microbiota in Women With Recurrent Spontaneous Abortion That Can Be Modified by Drug Treatment

Zhao

Chen

Gao

et al. 2021

Front. Cell. Infect. Microbiol.

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ObjectiveThe role of vaginal microbiota in recurrent spontaneous abortion (RSA) remains unknown. The purpose of this study was to investigate characteristics of vaginal microbiota and the effects of drug treatment on vaginal microbiota of patients with RSA.MethodsA case-control study was performed, in which non-pregnant patients who experienced RSA were selected and divided into untreated and drug-treated groups. Drug-treated patients were subdivided into the metformin group, metformin plus aspirin group, and other drugs group. Healthy women who had live births and never experienced spontaneous abortion were enrolled in the control group. Characteristics of vaginal microbiomes of patients with RSA and healthy women and the impact of drug treatment on the microbiome was evaluated via 16S rRNA gene sequencing of the V3-V4 region using the Illumina MiSeq platform.ResultsWomen who underwent RSA had lower microbial richness than healthy women. Compared to controls, the relative abundance of seven taxa (Megasphaera, Sneathia sanguinegens, Pseudomonas, Sphingomonas, Rhodococcus, Burkholderia- Caballeronia-Paraburkholderia, and Corynebacterium_1) in the patient’s vaginal microbiota changed significantly, which may be closely related to RSA. The composition of the vaginal microbial community in RSA patients was altered by drug treatment. Metformin combined with aspirin treatment significantly increased the relative abundance of vaginal Lactobacillus spp. in patients.ConclusionAn altered vaginal microbiome composition might be associated with RSA, which could be modified by drug treatment. The effect of metformin combined with aspirin on vaginal Lactobacillus is worthy of attention.

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Higher prevalence of colonization with Gardnerella vaginalis and gram-negative anaerobes in patients with recurrent miscarriage and elevated peripheral natural killer cells

Cited by 34 publications

References 34 publications

Alteration of vaginal microbiota in patients with unexplained recurrent miscarriage

Alteration of vaginal microbiota in patients with unexplained recurrent miscarriage

Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byGardnerella vaginalis

Characterization of Vaginal Microbiota in Women With Recurrent Spontaneous Abortion That Can Be Modified by Drug Treatment

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