Highlights on the current state of proteomic detection and characterization with nanopore sensors

Abstract

We believe this entire collection presents a wide overview of topics that are of growing interest to the nanopore sensing community. We have focused this issue on both biosensing applications of nanopore detection and fundamental mechanisms underlying polymer-pore interactions. We have identified a variety of investigators active in these pursuits and we hope that this will help further motivate studies in these areas. We thank each of the authors for their efforts along with the reviewers for making this special issue a reality, and we hope you, the reader, will benefit from the studies reported herein.

Procedural Data Processing for Single-Molecule Identification by Nanopore Sensors

“…Once the target molecules were driven into the pore, the baseline current instantaneously fluctuated in the manner of current spike pulses. These transient spike pulses could be categorized as either a current reducing event with electrolyte conductance decreasing or current enhancing events with electrolyte conductance increasing generated from molecule translocations [ 5 , 8 , 10 ]. Because weak pulse signals in the picoampere and nanoampere range emerge in ionic current traces with a strong noise background, it is challenging to recognize the pulse signals.…”

“…The monitored current pulses that are raised within the current baseline contribute to the physical features of the passing analytes and the dynamic interactions between the analytes and nanopores. The intensity, dwell time, capture frequency, and waveform of these current pulses can provide information on the dynamic changes in analytes including the volume, concentration, surface charge, and conformation features in solution [ 5 , 7 , 8 , 9 ]. Hence, it is important to collect and characterize the current pulses in nanopore measurements for a better understanding of the behavior of various analytes.…”