2021
DOI: 10.34133/2021/9756945
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Highly Dynamic Polynuclear Metal Cluster Revealed in a Single Metallothionein Molecule

Abstract: Human metallothionein (MT) is a small-size yet efficient metal-binding protein, playing an essential role in metal homeostasis and heavy metal detoxification. MT contains two domains, each forming a polynuclear metal cluster with an exquisite hexatomic ring structure. The apoprotein is intrinsically disordered, which may strongly influence the clusters and the metal-thiolate (M-S) bonds, leading to a highly dynamic structure. However, these features are challenging to identify due to the transient nature of th… Show more

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Cited by 10 publications
(13 citation statements)
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“…Moreover, this small protein domain's previously observed multiple unfolding pathways were detected and verified using our new method. 63,64 Fig. 3 A) The amino acid sequence of MT shows eleven endogenous cysteines.…”
Section: Resultsmentioning
confidence: 99%
See 3 more Smart Citations
“…Moreover, this small protein domain's previously observed multiple unfolding pathways were detected and verified using our new method. 63,64 Fig. 3 A) The amino acid sequence of MT shows eleven endogenous cysteines.…”
Section: Resultsmentioning
confidence: 99%
“…Moreover, this small protein domain's previously observed multiple unfolding pathways were detected and verified using our new method. 63,64…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…To examine the linker effect, we used atomic force microscopy-based single-molecule force spectroscopy (AFM-SMFS) to measure the mechanical stability of human Ig domains. SMFS can manipulate a single molecule mechanically [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ] and AFM-SMFS has been widely used to study the mechanical stability of proteins [ 23 , 24 , 25 , 26 , 27 , 28 , 29 ], protein-protein interactions [ 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ], and chemical bonds [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 ]. Many titin domains have been studied [ 57 , 58 , 59 ].…”
Section: Introductionmentioning
confidence: 99%