Highly efficient electrogenerated chemiluminescence quenching of PEI enhanced Ru(bpy)32+ nanocomposite by hemin and Au@CeO2 nanoparticles

Hong, Lin-Ru; Chai, Yaqin; Zhao, Min; Ni, Lan; Yuan, Ruo; Zhuo, Ying

doi:10.1016/j.bios.2014.07.065

Cited by 33 publications

(13 citation statements)

References 25 publications

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“…Furthermore, a comparative study was also conducted with other ECL aptasensors using Ru(bpy) 3 2 + and its derivatives as the luminescent substance for the detection of TB. As shown in Table 2 [11,14,15,24,28,[37][38][39][40], the proposed method performed comparable or even lower detection limit than other ECL aptasensors. It indicated that the combination of the PTG-AuNPs modified electrode and RuAg/SiO 2 NPs as signal-amplifying tags can effectively amplify the ECL signal and improve the sensitivity of the aptasensor, which makes the proposed sensing platform a reliable means of detection.…”

Section: Analytical Performance Of the Aptasensor To Tbmentioning

confidence: 81%

“…[Ru(phen) 2 (cpaphen)] 2 + /PEI CNTs-Nf/PEI@ DGNPs pNAMA-Ru-HGNP 1 fM-10 nM 0.54 fM [11] Ru(bpy) 3 2 + /TPrA Au/GO Ru-TBA II-AuNPs 0.01-10 nM 6.3 pM [14] Ru(bpy) 3 2 + /PEI Au/Ru-PEI-PAA Hemin/TBA/Au@ CeO 2 0.1 pM-10 nM 0.03 pM [28] Sandwich RuÀ SnS 2 /PAMAM Au@ZnO Au-PAMAM-RuÀ SnS 2 NP 5 fM-1 nM 2.6 fM [24] Ru(bpy) 3 2 + /Fc Ru(bpy) 3 2 + / β-cyclodextrinÀ Au/ GR Au/GR 0.4-1000 pM 0.23 pM [37] Ru(phen) 3…”

Section: Detection Limitmentioning

confidence: 99%

“…To date, the modification of Ru(bpy) 3 2 + is commonly divided into three types in TB assays. In the first case, Ru(bpy) 3 2 + is immobilized onto the electrode to form solid-state ECL substrate [26][27][28]. In most published papers, ion-exchangeable polymer (Nafion) films [26,27] are utilized for the immobilization of Ru(bpy) 3 2 + on the surface of the electrode of aptasensor.…”

Section: Introductionmentioning

confidence: 99%

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A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Shan

Gong

et al. 2019

Electroanalysis

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Herein, a signal‐on sandwich‐type electrochemiluminescence (ECL) aptasensor for the detection of thrombin (TB) was proposed. The graphene (GR) doped thionine (TH) was electropolymerized synchronously on the bare glassy carbon electrode (GCE) to form co‐polymer (PTG) electrode. The gold nanoparticles (AuNPs) were decorated on the surface of the PTG by in‐situ electrodeposition, and the functional co‐polymer (PTG‐AuNPs) electrode was utilized as sensing interface. Then, TB binding aptamer I (TBA I) as capture probes were modified on the PTG‐AuNPs electrode to capture TB, and Ru(bpy)32+/silver nanoparticles doped silica core‐shell nanocomposites‐labeled TB binding aptamer II (RuAg/SiO2NPs@TBA II) were used as signal probes to further bind TB, resulting in a sandwich structure. With the assistant of silica shell and AgNPs, the enrichment and luminous efficiency of Ru(bpy)32+ were significantly improved. Under the synergy of PTG‐AuNPs and RuAg/SiO2NPs, the ECL signal was dramatically increased. The proposed ECL aptasensor displayed a wide linear range from 2 fM to 2 pM with the detection limit of 1 fM, which is comparable or better than that in reported ECL aptasensors for TB using Ru(bpy)32+ and its derivatives as the luminescent substance. The excellent sensitivity makes the proposed aptasensor a promising potential in pharmaceutical and clinical analysis.

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Section: Analytical Performance Of the Aptasensor To Tbmentioning

confidence: 81%

Section: Detection Limitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Shan

Gong

et al. 2019

Electroanalysis

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“…While the enhanced ECL achieved as a result of thrombin capture by TBA1 aptamer anchored on nano-Au/Ru-PEI-PAA, the ECL signal was quenched after adding hemin/TBA2/Au@CeO 2 NPs. The quantification of thrombin was attained by measuring the difference between two ECL intensities for different concentrations of thrombin [173]. …”

Section: The Functions Of Mnps In Various Biosensor Typesmentioning

confidence: 99%

Noble metal nanoparticles in biosensors: recent studies and applications

Malekzad

Zangabad

Mirshekari

et al. 2017

Nanotechnology Reviews

303

119

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The aim of this review is to cover advances in noble metal nanoparticle (MNP)-based biosensors and to outline the principles and main functions of MNPs in different classes of biosensors according to the transduction methods employed. The important biorecognition elements are enzymes, antibodies, aptamers, DNA sequences, and whole cells. The main readouts are electrochemical (amperometric and voltametric), optical (surface plasmon resonance, colorimetric, chemiluminescence, photoelectrochemical, etc.) and piezoelectric. MNPs have received attention for applications in biosensing due to their fascinating properties. These properties include a large surface area that enhances biorecognizers and receptor immobilization, good ability for reaction catalysis and electron transfer, and good biocompatibility. MNPs can be used alone and in combination with other classes of nanostructures. MNP-based sensors can lead to significant signal amplification, higher sensitivity, and great improvements in the detection and quantification of biomolecules and different ions. Some recent examples of biomolecular sensors using MNPs are given, and the effects of structure, shape, and other physical properties of noble MNPs and nanohybrids in biosensor performance are discussed.

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“…28 As we all know, hemin could conjugate to a single-stranded guanine-rich nucleic acid named hemin/Gquadruplex DNAzymes with multiple mimic bioenzymes functions. 29,30 This multifunctional hemin/G-quadruplex DNAzyme has received tremendous interest in fluorescent, 30 electrochemical, 31−33 and colorimetric detection 34,35 as a catalytic amplification tag.…”

mentioning

confidence: 99%

Triple Quenching of a Novel Self-Enhanced Ru(II) Complex by Hemin/G-Quadruplex DNAzymes and Its Potential Application to Quantitative Protein Detection

Zhao

Zhuo

et al. 2015

Anal. Chem.

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Herein, a novel "on-off" electrochemiluminescence (ECL) aptasensor for highly sensitive determination of thrombin has been constructed based on the triple quenching of the effect of hemin/G-quadruplex DNAzymes upon the Ru(II) complex-based ECL system. First, a strong initial ECL signal was achieved by the dual amplification strategies of (i) intramolecular coreaction of a self-enhanced Ru(II)-based molecule (PTCA-PEI-Ru(II)) and (ii) intermolecular coreaction between PTCA-PEI-Ru(II) and nicotinamide adenine dinucleotide (NADH), which was named the signal-on state. Then, a novel triple quenching of the effect of multifunctional hemin/G-quadruplex DNAzymes upon the Ru(II) complex-based ECL system was designed to realize the desirable signal-off state, which was outlined as follows: (i) the hemin/G-quadruplex DNAzymes mimicked NADH oxidase to oxidize NADH and in situ generate the H2O2, consuming the coreactant of NADH; (ii) its active center of hemin could oxidize the excited state PTCA-PEI-Ru(II)* to PTCA-PEI-Ru(III), making the energy and electron transfer quench; (iii) it also acted as horseradish peroxidase (HRP) to catalyze the H2O2 for in situ producing the quencher of O2. Based on triple quenching of the effect of hemin/G-quadruplex DNAzymes, the highly sensitive "on-off" thrombin aptasensor was developed with a wide linear detection range of 1.0 × 10(-14) M to 1.0 × 10(-10) M and a detection limit down to the femtomolar level.

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Highly efficient electrogenerated chemiluminescence quenching of PEI enhanced Ru(bpy)32+ nanocomposite by hemin and Au@CeO2 nanoparticles

Cited by 33 publications

References 25 publications

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

Noble metal nanoparticles in biosensors: recent studies and applications

Triple Quenching of a Novel Self-Enhanced Ru(II) Complex by Hemin/G-Quadruplex DNAzymes and Its Potential Application to Quantitative Protein Detection

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Highly efficient electrogenerated chemiluminescence quenching of PEI enhanced Ru(bpy)32+ nanocomposite by hemin and Au@CeO2 nanoparticles

Cited by 33 publications

References 25 publications

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)32+ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)32+ Doped Nanocomposites as Signal‐amplifying Tags

Noble metal nanoparticles in biosensors: recent studies and applications

Triple Quenching of a Novel Self-Enhanced Ru(II) Complex by Hemin/G-Quadruplex DNAzymes and Its Potential Application to Quantitative Protein Detection

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A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags

A Sandwich‐type Electrochemiluminescence Aptasensor for Thrombin Based on Functional Co‐polymer Electrode Using Ru(bpy)₃²⁺ Doped Nanocomposites as Signal‐amplifying Tags