Highly efficient plant regeneration via somatic embryogenesis from cell suspension cultures of Boesenbergia rotunda

Wong, Sher Ming; Salim, Nursafina; Harikrishna, Jennifer Ann; Khalid, Norzulaani

doi:10.1007/s11627-013-9570-4

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…Similar to our study, Mathur and Shekhawat (2013) recorded the exponential phase at day 14 of culture in CSC of S. rebaudiana, and Kshirsagar et al (2015) at day 15 of CSC of Swertia lawii. However, some CSC needs a longer culture period (Shi et al 2013;Wong et al 2013;Qi et al 2014). Separately, cell viability was stable at around 82% over the 16 days of culture.…”

Section: Discussionmentioning

confidence: 99%

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Ramírez-Mosqueda

Iglesias-Andreu

2017

3 Biotech

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The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0.45 µM thidiazuron (TDZ). The effect of benzyladenine (BA) in different concentrations was evaluated. Cultures were incubated under photoperiod at continuous stirring at 120 rpm on an orbital shaker. The optimal condition found for biomass growth in suspension cultures was 0.5 g of inoculum density (fresh weight) in MS liquid, supplemented with 8.88 µM BA. The growth kinetics of the cell suspension culture revealed a maximum cell growth (exponential growth phase) at day 16 and an 80% cell viability. The establishment and characterization of cell suspension cultures of V. planifolia constitute the bases of future studies and above all a better biotechnological use of this crop.

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Section: Discussionmentioning

confidence: 99%

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Ramírez-Mosqueda

Iglesias-Andreu

2017

3 Biotech

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“…Cell suspension cultures were established according to Wong et al (2013). In brief, newly emerged buds obtained from the fresh rhizomes of B. rotunda were cultured on callus induction medium consisting of Murashige and Skoog (MS) salts (Murashige & Skoog 1962), 1.0 mg l −1 α-naphthaleneacetic acid (NAA), 1.0 mg l −1 6-benzyladenine (BA), 1.0 mg l −1 indole-3-acetic acid (IAA), 3% sucrose and 0.2% Gelrite ® (Sigma, St Louis, MO, USA).…”

Section: Plant Materials and Establishment Of Cell Suspension Culturementioning

confidence: 99%

Stable integration of mgfp5 transgenes following Agrobacterium-mediated transformation in Boesenbergia rotunda cell suspension culture

Wong

Zoolkefli

Karim

et al. 2015

Frontiers in Life Science

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Boesenbergia rotunda, a herb in the ginger family, contains numerous beneficial compounds, such as flavonoids, flavones and cyclohexenyl chalcone derivatives, that have great potential for pharmaceutical applications. However, the low concentration of the bioactive compounds limits their commercial application. In this study, a simple and reliable Agrobacterium-mediated transformation protocol for B. rotunda cell suspension culture was successfully developed. The minimal inhibitory concentration and natural tolerance of the selective agent, hygromycin, against the cells were 20 mg l −l and 30 mg l −l in liquid media and solid media, respectively. The highest number of transformed regenerants (18 ± 0.00 per ml settled cell volume) was recorded when cells were infected with Agrobacterium tumefaciens harbouring pCAMBIA1304 for 10 min and co-cultivated for 2 days. Prolonged infection time ( > 10 min) and co-cultivation period ( > 2 days), however, did not increase the transformation efficiencies. The results clearly show that infection and co-cultivation periods strongly influenced the transformation efficiency in ginger. The transformed cells were recovered and showed green fluorescent signals under ultraviolet excitation. An intense blue colour was observed in the transformed cells after β-glucuronidase (GUS) histochemical staining, further confirming the functionality of the GUS enzymes in the regenerants. Polymerase chain reaction analysis of 3-, 6-, 9-and 12-month-old transformed cells confirmed that the protocol enabled stable integration of the mgfp5 gene. Moreover, the comparatively high number of transformed regenerants in this study made it possible to generate a large number of transgenic cells in a short period, which would be useful for high-throughput functional screening of enzymes involved in the biosynthetic pathways of bioactive compounds.

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“…Several species of Zingiberaceae consisting mainly of herbaceous perennial plants are used in food (as spices), as well as ornamental plants, in cosmetics, and dyes. The rhizomes and leaves (Figure 4) are characterized by the presence of essential oils, flavones, flavonoids, and cyclohexenyl chalcone derivatives [47,48]. The rhizome is traditionally used, in Indonesia, for the treatment of several diseases in folk medicine owing to its diverse pharmacological and biological activities such as antibacterial, antifungal, antioxidant, anti-inflammatory, and anti-cancer [46].…”

Section: Temu Kunci (Boesenbergia Rotunda)mentioning

confidence: 99%

Ethnomedicinal Uses of Some Common Malaysian Medicinal Plants

Ahmed¹

2022

Natural Drugs From Plants

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Historically, natural products have always been a rich source of novel pharmacological leads, thus, making the ethnobotanical and ethnopharmacological knowledge an important and major asset of the medicinal plant-based drug discovery in providing hints for effective and safe chemotherapeutic compounds. Such knowledge, however, requires a thorough review and documentation of the ethnomedicinal and indigenous uses of local plants of every part of the world. Malaysia is a global hub for natural products which contributes to its GDP. Malaysia is also one of the 12 most diverse Megabiodiversity countries. Its rainforest is ranked 4th on the list of biodiversity hotspots in Asia after India, China, and Indonesia and is also acknowledged as the world’s oldest rainforest. Natural products are relevant to both Malaysian health care and agriculture which are parts of the national key economic areas (NKEA) under the Government’s Economic Transformation Plan as well as parts of the United Nations’ 17 Sustainable Development Goals (SDGs). Though the quality, efficacy, and safety of herbal products require global and international standardization, herbal products should be made accessible to low-income and rural communities across the globe. The proper documentation of the ethnopharmacological important plants in addition to their preservation and cultivation would enhance the sustainable use of the various indigenous plants. This work is unique in the sense that it is meant to review some of the most important high-value herbal products in Malaysia. Though it covers only a few representatives of Malaysian medicinal plants.

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Highly efficient plant regeneration via somatic embryogenesis from cell suspension cultures of Boesenbergia rotunda

Cited by 13 publications

References 29 publications

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Stable integration of mgfp5 transgenes following Agrobacterium-mediated transformation in Boesenbergia rotunda cell suspension culture

Ethnomedicinal Uses of Some Common Malaysian Medicinal Plants

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