Vanilla (Vanilla planifolia Jacks.) cell suspension cultures: establishment, characterization, and applications

Abstract

The establishment and characterization of cell suspension cultures are an in vitro culture technique very useful for various plant biotechnological applications (production of secondary metabolites, mass micropropagation, protoplast isolation and fusion, gene transfer and the investigation of cell pathways). The objective of this study was to establish and characterization of cell suspension cultures of V. planifolia by inducing friable calluses. For that, friable calluses were obtained from immature seeds cultivated in MS medium supplemented with 0.45 µM thidiazuron (TDZ). The effect of benzyladenine (BA) in different concentrations was evaluated. Cultures were incubated under photoperiod at continuous stirring at 120 rpm on an orbital shaker. The optimal condition found for biomass growth in suspension cultures was 0.5 g of inoculum density (fresh weight) in MS liquid, supplemented with 8.88 µM BA. The growth kinetics of the cell suspension culture revealed a maximum cell growth (exponential growth phase) at day 16 and an 80% cell viability. The establishment and characterization of cell suspension cultures of V. planifolia constitute the bases of future studies and above all a better biotechnological use of this crop.

Establishment of the Carrot-Made LTB-Syn Antigen Cell Line in Shake Flask and Airlift Bioreactor Cultures

“…We found a suitable cell growth using liquid MS basal medium supplemented with 2,4-D (2 mg/L) and kinetin (2 mg/L) for carrot cell suspension. Our results are consistent with other studies of cell suspension cultures 22 . In addition, our study contrasts with others that used BA (3 mg/L) and 1-naphthylacetic acid (0.2 mg/L) for transgenic carrots cultures to produce a vaccine candidate against Helicobacter pylori 23 .…”

Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

“…For example, the Oryza sativa embryonic suspension cell line was successfully induced in 1989. , Plant genotypes are closely related to the establishment of suspension cell line, and only some genotypes of Oryza sativa can be successfully induced . Besides, the monocotyledonous plant suspension cells reported include Veratrum california , Vanilla planifolia , Fritillaria cirrhosa , Dioscorea deltoidea , and Crocus sativus , etc. Gymnosperms are more difficult to induce than angiosperms because they are less sensitive to induction, and only few reports have been published so far, such as Taxus chinensis , Ginkgo biloba , Pinus pinaster .…”

Phenolic profile and antioxidant activity from wild and in vitro cultivated Rhynchostele rossii (Orchidaceae)

“…Although vanilla flavouring can now be produced by synthetic approaches, natural sources of these compounds are preferred by consumers around the world due to the flavour contribution of other compounds different from vanillin and vanillic acid. This has been one of the main reasons why research groups are interested in the development of culturing strategies for V. planifolia either in in or ex vitro conditions (Divakaran and Babu, 2009;Ramírez-Mosqueda and Iglesias-Andreu, 2017). A similar phenolic profile found in in vitro cultivated plants represents the capacity and opportunity that in vitro culture techniques offer to produce secondary metabolites of interest like wild plants do (Guerriero et al, 2018).…”