Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

Möller, Karin; Müller, K.; Engelke, Hanna; Bräuchle, Christoph; Wagner, Ernst; Bein, Thomas

doi:10.1039/c5nr06246b

Cited by 102 publications

(80 citation statements)

References 37 publications

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“…Actually, NPs can sufficiently disperse in aqueous solution and, thanks to their dimensions ranging from 1 to 200 nm, have more changes to avoid the activation of the complement cascade and their eventual clearance by immune cells and macrophages [20]. The fast nanotechnological evolution in the production of different kinds of NPs supports early disease diagnosis and staging, thus enabling also image-guided therapy and personalized therapy [192][193][194][195][196]. NPs application in theranostics allows to integrate diagnostic and therapeutic capabilities into a single nanostructure.…”

Section: Clinical Applications Of Nps Assisted Ultrasound In Cancer Tmentioning

confidence: 99%

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Canavese

Ancona

Racca

et al. 2018

Chemical Engineering Journal

351

247

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• Ultrasound-based therapies are opening new horizons in the oncological field.• Innovative and promising solutions derive from different nanoparticles-assisted ultrasound treatments.• Sonodynamic therapy (SDT) emerged recently as a novel approach for cancer treatment.• Different and complex cell death mechanisms are involved in nanoparticles-assisted SDT.• Nanoparticles-assisted ultrasound is still at its infancy in clinics. A R T I C L E I N F O Keywords:Inertial cavitation Reactive oxygen species Sonoluminescence Sonodynamic Tumor Therapy Cytotoxicity A B S T R A C TAt present, ultrasound radiation is broadly employed in medicine for both diagnostic and therapeutic purposes at various frequencies and intensities. In this review article, we focus on therapeutically-active nanoparticles (NPs) when stimulated by ultrasound. We first introduce the different ultrasound-based therapies with special attention to the techniques involved in the oncological field, then we summarize the different NPs used, ranging from soft materials, like liposomes or micro/nano-bubbles, to metal and metal oxide NPs. We therefore focus on the sonodynamic therapy and on the possible working mechanisms under debate of NPs-assisted sonodynamic treatments. We support the idea that various, complex and synergistics physical-chemical processes take place during acoustic cavitation and NP activation. Different mechanisms are therefore responsible for the final cancer cell death and strongly depends not only on the type and structure of NPs or nanocarriers, but also on the way they interact with the ultrasonic pressure waves. We conclude with a brief overview of the clinical applications of the various ultrasound therapies and the related use of NPs-assisted ultrasound in clinics, showing that this very innovative and promising approach is however still at its infancy in the clinical cancer treatment.

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Section: Clinical Applications Of Nps Assisted Ultrasound In Cancer Tmentioning

confidence: 99%

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Canavese

Ancona

Racca

et al. 2018

Chemical Engineering Journal

351

247

View full text Add to dashboard Cite

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“…[ 40 ] By simply mixing solutions containing Al 3+ ions and TA with the siRNA loaded nanoparticles, and adjusting the reaction pH, the surface coating is formed in minutes. In contrast to previous carrier coating strategies using lipid bilayer or cationic polymers, [ 4,8,30,41 ] this TA coating has been featured by high biocompatibility, availability to various substrates, facile one-step preparation and pH-dependent decomposition. [ 40,42,43 ] In this study, the successful coating of TA onto the nanoparticles is fi rst confi rmed by FTIR, X-ray photoelectron spectroscopy (XPS), thermogravimetric analysis ( Figure S7, Supporting Information).…”

Section: Loading Capacity and Protection Of Sirnamentioning

confidence: 95%

Magnetic Core–Shell Silica Nanoparticles with Large Radial Mesopores for siRNA Delivery

Xiong

Tang³

et al. 2016

Small

102

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A novel type of magnetic core-shell silica nanoparticles is developed for small interfering RNA (siRNA) delivery. These nanoparticles are fabricated by coating super-paramagnetic magnetite nanocrystal clusters with radial large-pore mesoporous silica. The amine functionalized nanoparticles have small particle sizes around 150 nm, large radial mesopores of 12 nm, large surface area of 411 m(2) g(-1) , high pore volume of 1.13 cm(3) g(-1) and magnetization of 25 emu g(-1) . Thus, these nanoparticles possess both high loading capacity of siRNA (2 wt%) and strong magnetic response under an external magnetic field. An acid-liable coating composed of tannic acid can further protect the siRNA loaded in these nanoparticles. The coating also increases the dispersion stability of the siRNA-loaded carrier and can serve as a pH-responsive releasing switch. Using the magnetic silica nanoparticles with tannic acid coating as carriers, functional siRNA has been successfully delivered into the cytoplasm of human osteosarcoma cancer cells in vitro. The delivery is significantly enhanced with the aid of the external magnetic field.

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“…Mesoporous silica nanoparticles (MSNs) are a versatile nanocarrier format that has been used for the delivery of chemotherapeutic agents[5,6], oligonucleotides[7,8], and proteins[9–11]. MSNs feature a stable framework to load and shelter cargo from proteases and the immune system and combine high cellular uptake efficiency with flexible surface functionalisation.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus

Chiu

Bates

Helma

et al. 2018

Nucleus

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Protein transfection is a versatile tool to study or manipulate cellular processes and also shows great therapeutic potential. However, the repertoire of cost effective techniques for efficient and minimally cytotoxic delivery remains limited. Mesoporous silica nanoparticles (MSNs) are multifunctional nanocarriers for cellular delivery of a wide range of molecules, they are simple and economical to synthesize and have shown great promise for protein delivery. In this work we present a general strategy to optimize the delivery of active protein to the nucleus. We generated a bimolecular Venus based optical sensor that exclusively detects active and bioavailable protein for the performance of multi-parameter optimization of protein delivery. In conjunction with cell viability tests we maximized MSN protein delivery and biocompatibility and achieved highly efficient protein transfection rates of 80%. Using the sensor to measure live-cell protein delivery kinetics, we observed heterogeneous timings within cell populations which could have a confounding effect on function studies. To address this problem we fused a split or dimerization dependent protein of interest to chemically induced dimerization (CID) components, permitting control over its activity following cellular delivery. Using the split Venus protein we directly show that addition of a small molecule dimerizer causes synchronous activation of the delivered protein across the entire cell population. This combination of cellular delivery and triggered activation provides a defined starting point for functional studies and could be applied to other protein transfection methods.

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Highly efficient siRNA delivery from core–shell mesoporous silica nanoparticles with multifunctional polymer caps

Cited by 102 publications

References 37 publications

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Nanoparticle-assisted ultrasound: A special focus on sonodynamic therapy against cancer

Magnetic Core–Shell Silica Nanoparticles with Large Radial Mesopores for siRNA Delivery

Nanoparticle mediated delivery and small molecule triggered activation of proteins in the nucleus

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