Highly electrocatalytic biosensor based on Hemin@AuNPs/reduced graphene oxide/chitosan nanohybrids for non-enzymatic ultrasensitive detection of hydrogen peroxide in living cells

Wang, Wenjing; Tang, Huabiao; Wu, Yuan; Zhang, Yinli; Li, Zhaohui

doi:10.1016/j.bios.2019.02.039

Cited by 47 publications

(20 citation statements)

References 40 publications

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“…Similar to semisynthetic MPs, other artificial systems like mimochromes [261][262][263][264], synthetic porphyrins, or hemin [265][266][267][268][269], showed reactivity toward H 2 O 2 and have been used as biological component in DET-based biosensors.…”

Section: Cytochrome C and Microperoxidasesmentioning

confidence: 99%

Electrocatalysis by Heme Enzymes—Applications in Biosensing

et al. 2021

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Heme proteins take part in a number of fundamental biological processes, including oxygen transport and storage, electron transfer, catalysis and signal transduction. The redox chemistry of the heme iron and the biochemical diversity of heme proteins have led to the development of a plethora of biotechnological applications. This work focuses on biosensing devices based on heme proteins, in which they are electronically coupled to an electrode and their activity is determined through the measurement of catalytic currents in the presence of substrate, i.e., the target analyte of the biosensor. After an overview of the main concepts of amperometric biosensors, we address transduction schemes, protein immobilization strategies, and the performance of devices that explore reactions of heme biocatalysts, including peroxidase, cytochrome P450, catalase, nitrite reductase, cytochrome c oxidase, cytochrome c and derived microperoxidases, hemoglobin, and myoglobin. We further discuss how structural information about immobilized heme proteins can lead to rational design of biosensing devices, ensuring insights into their efficiency and long-term stability.

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Section: Cytochrome C and Microperoxidasesmentioning

confidence: 99%

Electrocatalysis by Heme Enzymes—Applications in Biosensing

et al. 2021

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“…An alternative method for the development of redox-protein-based electrochemical sensors for live cells was proposed by Wang et al [166], who modified an electrochemical sensor with a composite of hemin, AuNPs, and reduced GO. In this case, they also used chitosan due to its outstanding permeability and high adhesiveness, which could form a stable film on top of the electrode surface.…”

Section: Electrochemical Techniques For Hydrogen Peroxide (H 2 O 2 ) Detectionmentioning

confidence: 99%

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

et al. 2021

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Reactive oxygen species (ROS) have been found in plants, mammals, and natural environmental processes. The presence of ROS in mammals has been linked to the development of severe diseases, such as diabetes, cancer, tumors, and several neurodegenerative conditions. The most common ROS involved in human health are superoxide (O2•−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Organic and inorganic molecules have been integrated with various methods to detect and monitor ROS for understanding the effect of their presence and concentration on diseases caused by oxidative stress. Among several techniques, fluorescence and electrochemical methods have been recently developed and employed for the detection of ROS. This literature review intends to critically discuss the development of these techniques to date, as well as their application for in vitro and in vivo ROS detection regarding free-radical-related diseases. Moreover, important insights into and further steps for using fluorescence and electrochemical methods in the detection of ROS are presented.

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“…Key features of nanohybrids also have opened up a gate of chances for robust and unique sensor development in mental healthcare management 42 . Nanohybrids owe more than a few advantages like capability of enhancing the sensing sensitivity, to accelerate the ionic and electron mobility with greater kinetics, and to increase the capturing event between target and analytes 43 . Few researches were carried out using nanohybrids as sensing performance boosting material to detect stress biomarkers.…”

Section: Nanohybrids For Sensor Developmentmentioning

confidence: 99%

Current strategies with sensing technologies to eliminate stress cardiomyopathy

Sun

et al. 2021

Biotech and App Biochem

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Stress cardiomyopathy refers weakening of heart muscle due to the continuous stress. Generally, the severe status of stress cardiomyopathy has been revealed after damaging the muscles and measured by the physical changes in the heart system. To overcome this issue, biosensor can be used, which could eliminate the late identification stress cardiomyopathy. With biosensors, different stress markers such as epinephrine, dopamine, catecholamine, α‐amylase, norepinephrine, serotonin and cortisol have been identified by a wide range of developments. These biosensors are available from laboratory to industry at the ranges of nano to macrodevices. To merge with the identification of stress cardiomyopathy, the above strategies might be utilized properly and can aid to reduce the stress‐related problems. This overview gleaned the currently available biosensing methods and the associated biomarkers at various stages of the developments and implementations of stress cardiomyopathy.

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Highly electrocatalytic biosensor based on Hemin@AuNPs/reduced graphene oxide/chitosan nanohybrids for non-enzymatic ultrasensitive detection of hydrogen peroxide in living cells

Cited by 47 publications

References 40 publications

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Electrocatalysis by Heme Enzymes—Applications in Biosensing

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

Current strategies with sensing technologies to eliminate stress cardiomyopathy

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