Highly Ordered 3D Tissue Engineering Scaffolds as a Versatile Culture Platform for Nerve Cells Growth

Abstract

Tissue engineering scaffolds provide an encouraging alternative for nerve injuries due to their biological support for nerve cell growth, which can be used for neuronal repair. Nerve cells have been reported to be mostly cultured on 2D scaffolds that cannot mimic the native extracellular matrix. Herein, highly ordered 3D scaffolds are fabricated for nerve cell culture by melt electrospinning writing, the microstructures and geometries of the scaffolds could be well modulated. An effective strategy for scaffold surface modification to promote nerve cell growth is proposed. The effects of scaffolds with different surface modifications, viz., plasma treatment, single poly-D-lysine (PDL) coating after plasma treatment, single laminin (LM) coating after plasma treatment, double PDL and LM coatings after plasma treatment, on PC12 cell growth are evaluated. Experiments show the scaffold modified with double PDL and LM coatings after plasma treatment facilitated the growth of PC12 cells most effectively, indicating the synergistic effect of PDL and LM on the growth of nerve cells. This is the first systematic and quantitative study of the effects of different scaffold surface modifications on nerve cell growth. The above results provide a versatile culture platform for growing nerve cells, and for recovery from peripheral nerve injury.

Electric‐Field‐Driven Printed 3D Highly Ordered Microstructure with Cell Feature Size Promotes the Maturation of Engineered Cardiac Tissues

“…h) Standard deviation of the fiber diameter and fiber spacing versus designed fiber spacing. i) Comparison between the fiber diameter/fiber spacing obtained in this study with other state‐of‐the‐art results reported in the literature (Zou et al., [ 39 ] Kong et al., [ 40 ] Wang et al., [ 41 ] Bertlein et al., [ 42 ] Juliane et al., [ 43 ] Eichholz et al., [ 44 ] Li et al., [ 45 ] Hrynevich et al., [ 46 ] Zhang et al., [ 47 ] Chen et al., [ 48 ] Delalat et al., [ 49 ] He et al., [ 50 ] Gwiazda et al., [ 51 ] Lourdes et al., [ 52 ] Bas et al., [ 53 ] Fuchs et al., [ 54 ] Xie et al., [ 55 ] Hochleitner et al. [ 56 ] )…”

“…Finally, we introduced and compared the ratio of fiber diameter/fiber spacing with other state‐of‐the‐art results in the literature (Figure 3i) to demonstrate the ability to fabricate 3D highly ordered microstructures with a very small fiber spacing. Our method can achieve the largest ratios of fiber diameter/fiber spacing, that is, 0.29 (20 µm) and 0.25 (10 µm), which are much larger than the reported values [ 39–56 ] (Table S2, Supporting Information). For example, He et al.…”

Preparation and Characterization of a Photo-Crosslinked Methacryloyl-Collagen Composite Film to Promote Corneal Nerve Regeneration via Surface Grafting of Taurine Molecules

“…The detection of drug-release and drug-load behavior is critical for predicting in vivo performance for quality control [41]. Hence, the drug loading of ColMA-Tr film and the cumulative drug release at different time points (Figure 5) were detected.…”