Highly pathogenic avian influenza virus subtype H5N1 in Mute swans in the Czech Republic

Nagy, Alexander; Machova, Jirina; Horníčková, Jitka; Tomci, Miroslav; Nagl, Ivan; Horyna, B; Holko, Ivan

doi:10.1016/j.vetmic.2006.10.004

Cited by 47 publications

(43 citation statements)

References 21 publications

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“…Another species of interest with respect to HPAI subtype H5N1 virus spread is the Mute Swan (Cygnus olor). The first incursion of HPAI subtype H5N1 virus in the Czech Republic was detected in this species [19]. Although the distribution of C. olor includes Romania, most of this species breeding in the Black Sea region undertake only limited migratory movements, leaving inland breeding 4 areas to winter on the coasts of the Black Sea [23].…”

Section: Discussionmentioning

confidence: 99%

Geostatistical visualisation and spatial statistics for evaluation of the dispersion of epidemic highly pathogenic avian influenza subtype H5N1

et al. 2008

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-The aim of this study was to evaluate a range of statistical and geostatistical methods for their usefulness in providing insights into how highly pathogenic avian influenza (HPAI) subtype H5N1 might spread through a national population of village poultry. The insights gained allow the generation of disease dispersion hypotheses. The case study data set consisted of 161 outbreaks of HPAI subtype H5N1 in village poultry reported in Romania between

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Section: Discussionmentioning

confidence: 99%

Geostatistical visualisation and spatial statistics for evaluation of the dispersion of epidemic highly pathogenic avian influenza subtype H5N1

et al. 2008

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“…In the Czech Republic the mute swan was also infected (Nagy et al, 2007) and, in this study, we made an attempt to estimate the amount of live avian influenza virus in organs available from the swans that had died of H5N1 avian influenza infection.…”

Section: Discussionmentioning

confidence: 99%

“…During the summer of 2005 the H5N1 virus was transmitted by migrating water birds to Europe, particularly to Rusia, Ukraine and Turkey (OIE, 2005a). In the winter months of 2006, the virus gradually spread to central, western and northern Europe (OIE, 2005b), including the Czech Republic (Nagy et al, 2007). The virus was also detected in the Middle East and in the north of Africa.…”

mentioning

confidence: 99%

“…In that period in the Czech Republic, 14 cases of avian influenza H5N1 were detected and only the mute swan was involved (Nagy et al, 2007). It remains to be found out whether the mute swan is an exceptionally susceptible species of water birds, or whether factors other than infection with H5N1 avian influenza virus are also responsible, and whether the swan may jeopardize other wild animals by shedding the H5N1 virus in the environment.…”

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confidence: 99%

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Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Rosenbergová¹,

Lány²,

Pospíšil³

et al. 2009

Vet. Med. - Czech

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This study reports on the first quantification of avian influenza virus in the organs of mute swans that died during the epizootic of avian influenza (H5N1) between January and April 2006 in the Czech Republic. The quantitative real-time Reverse Transcriptase PCR (qRT-PCR) assay based on a TaqMan probe was developed for a rapid detection and quantification of avian influenza virus RNA in clinical samples collected from mute swans. Conserved regions in the matrix protein gene of avian influenza virus served as targets for the primers and TaqMan probe design. A recombinant plasmid containing the matrix protein gene amplicon was constructed for a quantitative assay of copy numbers of the target gene. Quantification of avian influenza virus RNA was accomplished using a standard curve generated from ten-fold serial dilutions of recombinant plasmid DNA in the range of 10 2 to 10 8 copies/µl. Avian influenza virus A/Cygnus olor/Brno-cz/2006 was adapted to grow in VERO cells. In the same passage of cell cultivation, the concentration of viral RNA was determined to be 1.01 × 10 7 copies/ml and TCID 50 was 10 4.2 /ml. From these values the ratio of one RNA copy to 0.00157 virion capable of VERO cells infection was calculated. This ratio was used to estimate the virus concentrations in the tissues of dead mute swans.

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“…Highly pathogenic avian influenza H5N1 viruses can spread through flyways during bird migration because wild waterfowl are potential long-distance avian influenza virus (AIV) vectors (Keawcharoen et al, 2008). Since 2003, the spread of Asianorigin HPAI H5N1 into Europe and Africa has increased the focus on wild birdassociated AIV transmission (Terregino et al, 2006;Nagy et al, 2007;Gall-Recule et al, 2008).…”

mentioning

confidence: 99%

Surveillance and Isolation of HPAI H5N1 from Wild Mandarin Ducks (Aix galericulata)

Lee

Park

Youn

et al. 2011

Journal of Wildlife Diseases

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Highly pathogenic avian influenza virus subtype H5N1 in Mute swans in the Czech Republic

Cited by 47 publications

References 21 publications

Geostatistical visualisation and spatial statistics for evaluation of the dispersion of epidemic highly pathogenic avian influenza subtype H5N1

Geostatistical visualisation and spatial statistics for evaluation of the dispersion of epidemic highly pathogenic avian influenza subtype H5N1

Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT-PCR

Surveillance and Isolation of HPAI H5N1 from Wild Mandarin Ducks (Aix galericulata)

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