Highly selective, reusable electrochemical impedimetric DNA sensors based on carbon nanotube/polymer composite electrode without surface modification

Jiang, Huaide; Lee, Eun-Cheol

doi:10.1016/j.bios.2018.07.037

Cited by 37 publications

(12 citation statements)

References 32 publications

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“…There are two main methods for the polymer modification: noncovalent attachment (polymer wrapping and absorption) and covalent attachment ("grafting") [37]. There is a range of studies on the effect of polymer modification on the electrochemical, mechanical, thermal, and rheological properties of CNTs [38][39][40][41]. In addition, polymer modification and hybridization with CNTs have been also actively studied with regard to bio-medical applications, particularly for bio-sensing [42][43][44].…”

Section: Carbon Arc-discharge Methodsmentioning

confidence: 99%

The Advances in Biomedical Applications of Carbon Nanotubes

Saliev

2019

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Unique chemical, physical, and biological features of carbon nanotubes make them an ideal candidate for myriad applications in industry and biomedicine. Carbon nanotubes have excellent electrical and thermal conductivity, high biocompatibility, flexibility, resistance to corrosion, nano-size, and a high surface area, which can be tailored and functionalized on demand. This review discusses the progress and main fields of bio-medical applications of carbon nanotubes based on recently-published reports. It encompasses the synthesis of carbon nanotubes and their application for bio-sensing, cancer treatment, hyperthermia induction, antibacterial therapy, and tissue engineering. Other areas of carbon nanotube applications were out of the scope of this review. Special attention has been paid to the problem of the toxicity of carbon nanotubes.

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Section: Carbon Arc-discharge Methodsmentioning

confidence: 99%

The Advances in Biomedical Applications of Carbon Nanotubes

Saliev

2019

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“…Our impedance analysis showed sensitivity on the femtomolar level, while other measurement methods using gold nanoparticles [27,28] or carbon nanotubes [29,30,31] have reported better sensitivity on the atto or zeptomolar levels. However, such systems have certain drawbacks, such as the requirement to modify a specific ligand on the detection surface, and thus cannot provide comprehensive and continuous measurements.…”

Section: Discussionmentioning

confidence: 60%

Single-Molecule Detection of DNA in a Nanochannel by High-Field Strength-Assisted Electrical Impedance Spectroscopy

Pungetmongkol

Yamamoto

2019

Micromachines

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Many researchers have fabricated micro and nanofluidic devices incorporating optical, chemical, and electrical detection systems with the aim of achieving on-chip analysis of macromolecules. The present study demonstrates a label-free detection of DNA using a nanofluidic device based on impedance measurements that is both sensitive and simple to operate. Using this device, the electrophoresis and dielectrophoresis effect on DNA conformation and the length dependence were examined. A low alternating voltage was applied to the nanogap electrodes to generate a high intensity field (>0.5 MV/m) under non-faradaic conditions. In addition, a 100 nm thick gold electrode was completely embedded in the substrate to allow direct measurements of a solution containing the sample passing through the gap, without any surface modification required. The high intensity field in this device produced a dielectrophoretic force that stretched the DNA molecule across the electrode gap at a specific frequency, based on back and forth movements between the electrodes with the DNA in a random coil conformation. The characteristics of 100 bp, 500 bp, 1 kbp, 5 kbp, 10 kbp, and 48 kbp λ DNA associated with various conformations were quantitatively analyzed with high resolution (on the femtomolar level). The sensitivity of this system was found to be more than about 10 orders of magnitude higher than that obtained from conventional linear alternating current (AC) impedance for the analysis of bio-polymers. This new high-sensitivity process is expected to be advantageous with regard to the study of complex macromolecules and nanoparticles.

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“…This electrode was used as the working electrode for the electrochemical DNA sensor. Compared to previous sensors based on pristine MWCNT/PDMS electrodes [27,28], the LOD decreased by approximately 1250 times, from 25 pM to 20 fM. The electrode was stably regenerated by ethanol and water cleaning, which indicated that the physical modification method was highly stable.…”

Section: Introductionmentioning

confidence: 78%

“…In these cases, the chemical surface modifications consist of multiple steps that link probe DNA and nanomaterials to the electrode surfaces [24][25][26] and require several reagents. To avoid the complexity of chemical processes, in previous studies, multi-walled carbon nanotube (MWCNT)/polydimethylsiloxane (PDMS) composite electrodes were fabricated for DNA detection, and MWCNTs were used as the main material for the electrode [27,28]. In contrast to GCE, the electrode can behave as a recognition layer for DNA sensors without chemical modification; in addition, it is flexible and easy to fabricate.…”

Section: Introductionmentioning

confidence: 99%

Physical Surface Modification of Carbon-Nanotube/Polydimethylsiloxane Composite Electrodes for High-Sensitivity DNA Detection

2021

Self Cite

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The chemical modification of electrode surfaces has attracted significant attention for lowering the limit of detection or for improving the recognition of biomolecules; however, the chemical processes are complex, dangerous, and difficult to control. Therefore, instead of the chemical process, we physically modified the surface of carbon-nanotube/polydimethylsiloxane composite electrodes by dip coating them with functionalized multi-walled carbon nanotubes (F-MWCNTs). These electrodes are used as working electrodes in electrochemistry, where they act as a recognition layer for sequence-specific DNA sensing through π–π interactions. The F-MWCNT-modified electrodes showed a limit of detection of 19.9 fM, which was 1250 times lower than that of pristine carbon/polydimethylsiloxane electrodes in a previous study, with a broad linear range of 1–1000 pM. The physically modified electrode was very stable during the electrode regeneration process after DNA detection. Our method paves the way for utilizing physical modification to significantly lower the limit of detection of a biosensor system as an alternative to chemical processes.

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Highly selective, reusable electrochemical impedimetric DNA sensors based on carbon nanotube/polymer composite electrode without surface modification

Cited by 37 publications

References 32 publications

The Advances in Biomedical Applications of Carbon Nanotubes

The Advances in Biomedical Applications of Carbon Nanotubes

Single-Molecule Detection of DNA in a Nanochannel by High-Field Strength-Assisted Electrical Impedance Spectroscopy

Physical Surface Modification of Carbon-Nanotube/Polydimethylsiloxane Composite Electrodes for High-Sensitivity DNA Detection

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