Highly selective separation and purification of chicoric acid from Echinacea purpurea by quality control methods in macroporous adsorption resin column chromatography

Abstract

Chicoric acid is the main phenolic active ingredient in Echinacea purpurea (Asteraceae), best known for its immune‐enhancing ability, as well as used as a herbal medicine. To achieve further utilization of medicinal ingredients from E. purpurea, an efficient preparative separation of chicoric acid was developed based on macroporous adsorption resin chromatography. The separation characteristics of several different typical macroporous adsorption resins were evaluated by adsorption/desorption column experiments, and HPD100 was revealed as the optimal one, which exhibited that the adsorbents fitted well to the pseudo‐second‐order kinetics model and Langmuir isotherm model, and the optimal process parameters were obtained. The breakthrough curves could be predicted and end‐point could be determined early. Besides, the optimal elution conditions of chicoric acid can be achieved using the quality control methods. As a result, the purity of chicoric acid was increased 15.8‐fold (from 4 to 63%) after the treatment with HPD100. The process of the enrichment and separation of chicoric acid is considerate, because of its high efficiency and simple operation. The established separation and purification method of chicoric acid is expected to be valuable for further utilization of E. purpurea according to product application in pharmaceutical fields in the future.

Increasing medicinal and phytochemical compounds of coneflower (Echinacea purpurea L.) as affected by NO3−/NH4+ ratio and perlite particle size in hydroponics

“…Plants may produce large amounts of phenolic compounds and phenolic derivatives in the roots in response to exceed nitrate accumulation 34 . According to Ghabaei et al 12 , one of the mitigating mechanisms of nitrogen excess stress is the accumulation of phenolic compounds in the plant.…”

“…4 ). The difference in the phenolic acid concentrations extracted from the plant roots depends on various factors such as environmental growth conditions (light, temperature, and humidity) and especially the growing period of the plant 34 . It is well known that the concentrations of caffeic acid derivatives is different during vegetative to reproductive stages 35 .…”

A novel online preparative high‐performance liquid chromatography system with the multiple trap columns‐valve switch technique for the rapid and efficient isolation of main flavonoids from Epimedium koreanum Nakai

“…Using this cutting procedure, the eluent was cut into 18 subfractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. The application of the eluent cutting procedure could effectively remove the interference from the surrounding eluent, centralize the separation of targets, and facilitate subsequent separation.…”

“…However, the complex chemical compositions of natural products generally limit their fast and effective isolation. Traditional preparative isolation systems, such as thin‐layer chromatography [3], macroporous resin column chromatography [4], silica gel column chromatography [5], and Sephadex column chromatography [6], have not been suitable for the rapid and efficient separation of the complex compositions of natural products isolated from plants and other organisms due to the low level of automated separation, low peak capacity, and weak separation and adsorption capacities. Therefore, the development of an ideal preparative isolation system is necessary for the efficient and rapid isolation of natural products from these complex isolates.…”

Simultaneous Purification and Separation of Syringoside and Oleuropein from Syringa oblata Lindl. Extract Using Macroporous Resin

“…However, these methods have a low separation efficiency, are time-consuming, consume large amounts of organic solvents, and cause environmental pollution; thus, their industrial application is limited. Combining the adsorption mechanism and screening principle of resins, different organic compounds can be separated according to their adsorption capacity and molecular weight [20,21]. Compared with other separation methods, the macroporous adsorption resin method has a larger adsorption capacity, better selectivity, faster adsorption, mild desorption conditions, convenient regeneration, simpler operation, lower cost, and reusability [22,23].…”