Highly sensitive and selective detection of biothiols by a new low dose colorimetric and fluorescent probe

Zhang, Qiong; Ding, Shuangshuang; Zhai, Qisong

doi:10.1039/c5ra11841g

Cited by 25 publications

(4 citation statements)

References 52 publications

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“…The cyano group is an electron acceptor, and the alkoxy group is an electron donor, making PFE-CN-2 with an electron donor–conjugated system–electron acceptor (D-π-A) structure. This structure makes the fluorescence enhancement of PFE-CN-2 and the maximal absorption/emission red-shifting about 6 nm compared to PFE-1, which is consistent with that previously reported in the literature. , Because of 2,4-dinitrobenzenesulfonyl, an electron-withdrawing group, being linked as a side chain of PFE-NP-3 (electron acceptor–conjugated system–electron acceptor, A-π-A), PFE-NP-3 fluorescence is quenched by photoinduced electron transfer (PET) effect, leading to the poorest fluorescence intensity. − To further compare the photophysical properties of the four kinds of CCPs, the absolute fluorescence quantum yield (Φ F ) in aqueous solution was measured. The cationic conjugated polymers were excited with the corresponding maximal absorption wavelength.…”

Section: Resultssupporting

confidence: 90%

Efficient Antibacterial Performance and Effect of Structure on Property Based on Cationic Conjugated Polymers

et al. 2018

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Cationic conjugated polymers (CCPs) have attracted more and more attention in antibacteria and tumor treatment based on photodynamic therapy (PDT). However, the main chain structure−antibacterial activities relationship has been rarely reported. Herein, we designed and synthesized four cationic conjugated polymers: one poly(fluorine phenylene) derivative (PFP) and three poly(fluorene-co-phenylene ethynylene) derivatives (PFE-1, PFE-CN-2, and PFE-NP-3). PFP and PFE-1 have the same side chains but bear different conjugated backbones. PFE-CN-2 (π-A) and PFE-NP-3 (A-π-A) are modified with electron-donating and/or electron-withdrawing groups. Three PFEs can produce reactive oxygen species (ROS) faster than PFP. The order of antibacterial activities of the four CCPs is as follows: PFE-CN-2 > PFE-1 ≫ PFE-NP-3 ≈ PFP, which is coincident with the generation rate of ROS. In addition, CCPs with D-π-A structure is more advantageous than A-π-A in ROS production and in antibacterial performance. These results provide an important base for designing more efficient PDT agents for antibacteria and antitumor.

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Section: Resultssupporting

confidence: 90%

Efficient Antibacterial Performance and Effect of Structure on Property Based on Cationic Conjugated Polymers

et al. 2018

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“…actively used as fluorescent probes for rapid detection of H2S [4], benzenethiols [5], biothiols [6], selenocysteine [7],…”

Section: -Benzothiazolylcoumarins Arementioning

confidence: 99%

Hetarenocoumarins based on 7-hydroxy-3-(benzothiazol-2-yl)coumarin

et al. 2021

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“…Among them, fluorescence detection approaches, owing to their advantages of excellent sensitivity, low cost, high spatiotemporal resolution, ease of observation and noninvasive detection, have attracted interest in biological imaging analysis, clinical disease diagnosis, food detection, and environmental detection [17][18][19][20]. At present, a number of fluorescent Molecules 2021, 26, 2337 2 of 12 probes have been designed for biothiols imaging [21][22][23][24][25][26][27][28][29]. Despite great efforts, some defects associated with current sensors, including low selectivity, complicated syntheses, low biocompatibility or a small Stokes shift (<100 nm), still remain.…”

Section: Introductionmentioning

confidence: 99%

A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

et al. 2021

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Due to the redox properties closely related to numerous physiological and pathological processes, biothiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), have received considerable attention in biological science. On account of the important physiological roles of these biothiols, it is of profound significance to develop sensitive and selective detection of biothiols to understand their biological profiles. In this work, we reported an efficient fluorescent probe, PHPQ-SH, for detecting biothiols in vitro and vivo, based on the phenothiazine-HPQ skeleton, with DNBS (2,4-dinitrobenzenesulfonate) as the response unit. Probe PHPQ-SH exhibited brilliant sensing performances toward thiols, including a large Stokes shift (138 nm), excellent sensitivity (for GSH, LOD = 18.3 nM), remarkable fluorescence enhancement (163-fold), low cytotoxicity, rapid response (8 min), and extraordinary selectivity. Finally, the probe PHPQ-SH illustrated herein was capable of responding and visualizing biothiols in MCF-7 cells and zebrafish.

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Highly sensitive and selective detection of biothiols by a new low dose colorimetric and fluorescent probe

Cited by 25 publications

References 52 publications

Efficient Antibacterial Performance and Effect of Structure on Property Based on Cationic Conjugated Polymers

Efficient Antibacterial Performance and Effect of Structure on Property Based on Cationic Conjugated Polymers

Hetarenocoumarins based on 7-hydroxy-3-(benzothiazol-2-yl)coumarin

A Phenothiazine-HPQ Based Fluorescent Probe with a Large Stokes Shift for Sensing Biothiols in Living Systems

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