Highly sensitive and selective surface plasmon resonance sensor for detection of sub-ppb levels of benzo[a]pyrene by indirect competitive immunoreaction method

Miura, Norio; Sasaki, M.; Gobi, K. Vengatajalabathy; Kataoka, Chiwa; Shoyama, Yukihiro

doi:10.1016/s0956-5663(02)00242-7

Cited by 83 publications

(40 citation statements)

References 19 publications

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“…However, the detection of small molecule clenbuterol (Mw = 277) by a mass transducer using the direct immunoassay is challenging. In this study, we employ the indirect competitive inhibition immunoassay for detecting the small analyte [6,[33][34][35][36]. This method ensures that the signal response of the mass transducer is large, because the antibody, rather than the antigen, is being measured.…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…An indirect competitive method, which is effective to detect chemical substances with low molecular weight, was used for highly sensitive detection (5)- (7) . A schematic diagram of the indirect competitive method is shown in Figure 2.…”

Section: Indirect Competitive Methodsmentioning

confidence: 99%

Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

et al. 2006

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“…Only a small fraction of the surface-bound antibodies was removed by the flow of either of these eluents for 2-4 min. With our previous experience [20][21][22], complete removal of the surface-bound antibodies, thus regeneration of active sensor surface, was achieved by the flow of pepsin (5 lg/ml in glycine-HCl buffer, pH 2.0) for a period of 30-60 s (Fig. 5, inset).…”

Section: Stability and Reusabilitymentioning

confidence: 99%

“…In this regard, we have earlier investigated antigenprotein conjugate bound sensor surfaces for the development of immunosensors in combination with an indirect competitive immunoassay principle for detection of analytes of LMW and of very low concentrations [19][20][21][22]. Immunoassays based on antigen-coat formats will be of high flexibility to the fabrication protocols and are interested because of the possibility to repeated use of a same sensor chip [13,[22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Highly regenerable and storageable all-chemical based PEG-immunosensor chip for SPR detection of ppt levels of fragrant compounds from beverage samples

Gobi

Matsumoto

Toko

et al. 2008

Sens. & Instrumen. Food Qual.

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Simple, automated and cost-effective sensor systems intended for detection of low-molecular-weight (LMW) fragrant compounds are indispensable at automated, bottle-filling production lines of food and beverage industries. Here, we report our investigations on the development of a highly regenerable and storageable surface plasmon resonance (SPR)-based biosensor for detection of trace amounts of benzaldehyde (BZ), a characteristic fragrant compound of peach products. The sensing surface was fabricated by self-assembling of longchain polyethyleneglycol(PEG)-dialkanethiols on thin Aufilms and by followed covalent-binding of a BZ analog on the self-assembly of PEG-dialkanethiols. The all-chemical based BZ-bound PEG-monolayer chip shows specific binding affinity toward anti-BZ antibody (BZ-Ab). The principle of indirect competitive immunoassay, promising highly sensitive and consistent detection of small molecular analytes, has been applied, wherein immunospecific binding of BZ-Ab onto the BZ-bound sensor surface is inhibited by analyte BZ present in test sample. The immunosensor exhibited excellent sensitivity for BZ detection over a wide concentration range of 0.1-80 ng/ml (ppb). The BZ-bound monolayer surface was highly regenerable and storageable for repeated use of a same sensor chip. The assay reproducibility was proved through sequential multiple analysis of the same sensor chip for more than 50 regeneration cycles. Sensitivity was enhanced further by an add-on strategy involving anti-(rabbit IgG)-functionalized Au nanoparticles (nAu-Ab), with which the sensor-signal is enhanced by about 15-18 times and the amount of BZ-Ab antibody required for an analysis could be reduced to one-tenth. With the enhancement technique, detection of as low as 7 pg/ml (ppt) BZ directly from beverage samples has been demonstrated.

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Highly sensitive and selective surface plasmon resonance sensor for detection of sub-ppb levels of benzo[a]pyrene by indirect competitive immunoreaction method

Cited by 83 publications

References 19 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Detection of Aromatic Nitro Compounds Using Preconcentrator and SPR Immunosensor

Highly regenerable and storageable all-chemical based PEG-immunosensor chip for SPR detection of ppt levels of fragrant compounds from beverage samples

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