Highly sensitive and surface-renewable electrochemical quartz crystal microbalance assays of heparin and chondroitin sulfate based on their effects on the electrodeposition of neutral red

Yu, Rongmin; Ma, Ming; Wang, Lingling; Xie, Qingji; Cao, Zhibo; Jiang, Xueqin; Yao, Shouzhuo

doi:10.1016/j.bios.2008.09.010

Cited by 11 publications

(4 citation statements)

References 39 publications

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“…Thus, more assays for heparin detection have been established, including fluorimetry, [31][32][33][34] light scattering, 35 and electrochemical methods. 36,37 Those methods still have some limitations such as complicated procedures, poor sensitivity, instruments, high-cost, or narrow applications. Therefore, it is urgently required for the detection of heparin to develop methods with the advantages of simplicity, rapidity, and cost-effectiveness.…”

Section: Introductionmentioning

confidence: 99%

A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(ii) complexes for heparin detection

Cheng

2017

Analyst

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A sensitive assay was demonstrated for the colorimetric and luminescent detection of heparin. Positively charged [Ru(phen)np] (np: naphtho[2,3-f][1,10]phenanthroline) can interact with negatively charged AuNPs through electrostatic interaction, leading to the luminescence quenching of [Ru(phen)np]via energy transfer as well as the aggregation of AuNPs together with a color change in solution from red to blue. Upon the addition of negatively charged heparin to gold nanoparticles functionalized by ruthenium(ii) complexes, the emission of [Ru(phen)np] recovered due to the competitive adsorption of [Ru(phen)np] to heparin and AuNPs. A linear calibration curve was obtained in the 0.4 μM to 100 μM range and 0.1 μM to 6 μM with a low detection limit of 0.22 μM and 0.024 μM based on 3σ/s for heparin quantification in buffer solution and in 1% fetal bovine serum (FBS), respectively. Interestingly, the naked eye alone can judge the presence of 0.02 μM heparin without the aid of any advanced instruments by color change. Thus a convenient and visual method to detect heparin was established.

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Section: Introductionmentioning

confidence: 99%

A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(ii) complexes for heparin detection

Cheng

2017

Analyst

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“…1 Therefore, sensors that can detect and quantify these metal ion and organic molecule targets have been developed for various applications. 2–6 In comparison with instrument analysis such as inductively coupled plasma (ICP) and mass spectrum, these sensors have the advantage that no complicated instrument or sample pretreatment is needed. Until recently antibody-based assays are a major methodology that is general enough for the development of sensors for many targets.…”

Section: Introductionmentioning

confidence: 99%

Label-Free Catalytic and Molecular Beacon Containing an Abasic Site for Sensitive Fluorescent Detection of Small Inorganic and Organic Molecules

et al. 2012

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In this work, two methods with complementary features, catalytic and molecular beacon (CAMB) and label-free fluorescent sensors using abasic site, have been combined into new label-free CAMB sensors that possess advantages of each method. The label-free method using dSpacer-containing molecular beacon makes CAMB more cost-effective and less interfering to the catalytic activity, while the CAMB allows the label-free method to use true catalytic turnovers for signal amplifications, resulting in a new label-free CAMB sensor for Pb2+ ion, with a detection limit of 3.8 nM while maintaining the same selectivity. Furthermore, by using CAMB to overcome the label-free method’s limitation of the requiring excess enzyme strand, a new label-free CAMB sensor using aptazyme is also designed to detect adenosine down to 1.4 μM, with excellent selectivity over other nucleosides.

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“…Close monitoring and determination of heparin is much important not only for its regulation in the physiological process but also for its clinical application during surgery and postoperative therapy period. Several methods for monitoring highly negatively charged polyanions have been developed for these polysaccharides in commercial or clinical samples (Fu et al, 2012;Sun et al, 2006;Yu et al, 2009). In recent years, researchers have developed some fluorescence approaches for polyanions detection by electrostatic interaction (Cai et al, 2011;Dai et al, 2011;Kim et al, 2014;Wu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

Sun

Shi

et al. 2015

Biosensors and Bioelectronics

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Highly sensitive and surface-renewable electrochemical quartz crystal microbalance assays of heparin and chondroitin sulfate based on their effects on the electrodeposition of neutral red

Cited by 11 publications

References 39 publications

A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(ii) complexes for heparin detection

A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(ii) complexes for heparin detection

Label-Free Catalytic and Molecular Beacon Containing an Abasic Site for Sensitive Fluorescent Detection of Small Inorganic and Organic Molecules

Multi-positively charged dendrimeric nanoparticles induced fluorescence quenching of graphene quantum dots for heparin and chondroitin sulfate detection

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