Highly-sensitive cholesterol biosensor based on well-crystallized flower-shaped ZnO nanostructures

Efficient detection of cholesterol using ZnO thin film based matrix

“…The variation is shown in Figure 7 giving a maximum interference of 4.4% [5,17], which may be attributed to highly stable ZnO thin film matrix prepared in the present case.…”

“…The value of K m is estimated to be 1.92 mM for the ChOx immobilised on the bio-electrode which is found to be comparable to the values reported for other cholesterol biosensors. Singh et al have reported a K m of 2.1 mM, while Umar et al reported 4.7 mM for their respective bioelectrodes [5,14]. The low value of K m reflects enhanced activity of ChOx enzyme towards its analyte (cholesterol) and is due to the favourable conformational changes in the enzyme's structure on being immobilised on the matrix, which is facilitated by the suitable biocompatible microenvironment provided by the ZnO thin film matrix prepared by the PLD technique.…”

“…Cholesterol biosensor is intensely investigated as high serum cholesterol level related to various clinical disorders, such as heart diseases, coronary artery disease, arteriosclerosis, hypertension and cerebral thrombosis [3][4][5][6]. A biosensor is a biomolecular electronic device that translates a biological response into an equivalent electrical signal.…”

“…ZnO is a wide band gap semiconducting oxide material which is biocompatible with a high isoelectric point of 9.5 (suitable for adsorption of proteins having low isoelectric point (IEP)) and has specific properties including ease of fabrication, high specific surface area, nontoxicity, chemical stability, strong adsorption ability and high electron communication feature [5][6][7][11][12][13][14], which make it an excellent matrix to be used in biosensors. ZnO thin films and nanostructures have been extensively used for the detection of various biomolecules, however to the best of our knowledge very little work has been done on cholesterol detection.…”

Cholesterol Oxidase: Source,Properties and Applications.

“…Most of the reported cholesterol biosensors are based on amperometric technique besides, cholesterol biosensors based on photometric behaviors like luminescence, fluorescence and surface plasmon resonance (SPR) have also been reported [88,114,115]. Amperometric measurement of O 2 consumption or H 2 O 2 production during cholesterol catalysis by CHOx is the frequently used strategy in cholesterol assay in the samples [116]. Various nanoparticles like metal nanoparticles (gold (Au), platinum (Pt), silver (Ag) NPs), metal oxide nanoparticles (zinc oxide (ZnO), iron oxide (Fe3O 4 ), cerium oxide (CeO 2 ), titanium oxide (TiO 2 ) NPs), carbon nanotubes (single-walled carbon nanotubes (SWCNTs), and multi-walled carbon nanotubes (MWCNTs) based materials have been exploited for designing cholesterol biosensor [117][118][119][120][121].…”