Highly Sensitive Detection of miRNA-155 Using Molecular Beacon-Functionalized Monolayer MoS₂ Nanosheet Probes with Duplex-Specific Nuclease-Mediated Signal Amplification

Abstract

MicroRNA-155 (miRNA-155) as a characteristic myeloma-associated biomarker exhibits significant potential application in the diagnosis of multiple myeloma (MM). In this paper, a novel type of molecular beacon (MB)-functionalized monolayer MoS2 nanosheet probe was proposed as fluorescent probe for high-sensitive assays of miRNA-155that uses a duplexspecificnuclease (DSN) enzyme to amplify the fluorescence signal. The preparation and detection conditions of the fluorescent probes were optimized in some aspects, such as the concentration of MoS2 (0.80 μM) and DSN (0.2 U), and the incubation time of DSN (30 min). The probesexhibited a sensitive fluorescence response to miRNA-155 and the fluorescence signal of the assay was significantly amplified by the cleavage of DSN. The relationship between F/F0 and logC miRNA follows a linear calibration curve, and the limit of detection (LOD) of miRNA-155 in 10% human serum is calculated to be 10.96 fM based on this relationship. The good performance and fluorescence amplification effect of the fluorescent probe were confirmed by studying the recovery of miRNA-155 in 10% human serum, which was ranged from 98.32% to 106.3% with a relative standard deviation of less than 4.14%. Besides, the high expression of miRNA-155 in clinic blood of MM patients was sensitively distinguished from healthy peoples by using the proposed probes. The proposed novel fluorescent probe based on the DSN can be used to detect miRNA-155 in human serum and provide a potential, convenient and reliable tool for diagnosis of MM.

The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

“…The results show the good anti-interference ability of probes for detecting miRNAs in complex samples. Liu Ying et al detected the fluorescence intensity of miRNA-155 in serum of healthy people and MM patients with MB-functionalized monolayer MoS 2 nanosheet fluorescent probe [ 40 ]. According to the experimental results, we can infer that the concentration of miRNA-155 in health people serum is about 53.7 pM, and that of miRNA-155 in MM patients serum is about 6.12 nM, which proves that the sensitivity and detection range achieved by our pattern method can meet the clinical detection requirements of miRNA.…”

MicroRNA-126-3P targets PIK3R2 to ameliorate autophagy and apoptosis of cortex in hypoxia–reoxygenation treated neonatal rats

“…The target gene regulated by miRNA accounts for at least 20% of the total genome, which involves the biological process of growth and development of various cells, and then regulates the pathophysiological process of many nervous system diseases. In the process of nervous system damage, there is abnormal inactivation or over-expression of miRNA (21)(22)(23). MiR-126-3p is specifically expressed in many systems, such as respiratory, digestive, hematopoietic, reproductive and embryonic tissues.…”