Highly Sensitive Detection using Dual Working Electrode and Concentration Process in Electrochemical Metalloimmunoassay

Hori, Nobuyasu; Chikae, Miyuki; Kirimura, Hiroya; Takamura, Yuzuru

doi:10.1016/j.electacta.2015.06.061

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…The typical size range of AgNS utilized in the past studies is between B5-100 nm. 19,20,24,26,27,30,33,35,42,80,115 For instance, Szymanski et al developed an immunosensor for detecting myoglobin, a cardiac heart marker, using AgNS (40 nm) as an electroactive label. 80 The AgNS were deposited onto a WE with help of an aggregating agent, NH 4 SCN, and a final anodic stripping voltammetry step was performed to detect the Ag oxidation current.…”

Section: Effect Of Agnp Shape and Size On The Ge Reactionmentioning

confidence: 99%

“…The oxidation current generated is directly correlated with the analyte concentration in the sample. Such AgNP-labeled sandwich-type immunoassays have been exploited to detect HBs 19 and the heart failure biomarker, N-terminal prohormone brain natriuretic peptide (NT-proBNP). 20 Also, some groups have employed more complex nanomaterials with AgNPs to improve the immunoassay sensitivity.…”

Section: Immunosensors Based On Direct Oxidation Of Agnp-labelsmentioning

confidence: 99%

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Silver nanoparticles in electrochemical immunosensing and the emergence of silver–gold galvanic exchange detection

Walgama¹,

Raj²

2023

Chem. Commun.

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Section: Effect Of Agnp Shape and Size On The Ge Reactionmentioning

confidence: 99%

Section: Immunosensors Based On Direct Oxidation Of Agnp-labelsmentioning

confidence: 99%

Silver nanoparticles in electrochemical immunosensing and the emergence of silver–gold galvanic exchange detection

Walgama¹,

Raj²

2023

Chem. Commun.

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“…It has been successfully employed in biosensing devices using SPEs [62,63]. An interesting approach was reported using a dual screen-printed working electrode device [64]. Working electrodes with different size were employed, and the biological reaction was carried out using both electrodes as heterogeneous surfaces.…”

Section: Strategies For Electrochemical Detection Of Nanoparticlesmentioning

confidence: 99%

Electrochemical Detection and Characterization of Nanoparticles with Printed Devices

Martín‐Yerga

2019

Biosensors

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Innovative methods to achieve the user-friendly, quick, and highly sensitive detection of nanomaterials are urgently needed. Nanomaterials have increased importance in commercial products, and there are concerns about the potential risk that they entail for the environment. In addition, detection of nanomaterials can be a highly valuable tool in many applications, such as biosensing. Electrochemical methods using disposable, low-cost, printed electrodes provide excellent analytical performance for the detection of a wide set of nanomaterials. In this review, the foundations and latest advances of several electrochemical strategies for the detection of nanoparticles using cost-effective printed devices are introduced. These strategies will equip the experimentalist with an extensive toolbox for the detection of nanoparticles of different chemical nature and possible applications ranging from quality control to environmental analysis and biosensing.

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Design of dual working electrodes for concentration process in metalloimmunoassay

Hori

Chikae

Kirimura

et al. 2016

Biomed Microdevices

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Electrochemical immunosensing, particularly through a metalloimmunoassay, is a promising approach for development of point-of-care (POC) diagnostics devices. This study investigated the structure of dual working electrodes (W1 and W2), used in a silver nanoparticles-labeled sandwich-type immunoassay and silver concentration process, paying special attention to the position of W1 relative to W2. The new structures of the dual working electrodes were fabricated for efficient silver concentration and evaluated experimentally, which showed that the duration of prereduction before current measurement decreased from 480 s to 300 s by transforming the position of W1 from 1 line to 2 lines or 6 parts. The experimental results were also compared with numerical simulations based on three-dimensional diffusion, and the prereduction step almost followed the three-dimensional diffusion equation. Using numerical simulations, the ideal structures of dual working electrodes were designed based on relationships between the structures and duration of prereduction or the LOD. In the case of 36 lines at an area ratio of W1 to W1 + W2 of 1 to 10, the prereduction duration decreased to 96 s. The dual working electrodes designed in this study promise to shorten the total analysis time and lower the LOD for POC diagnostics.

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Highly Sensitive Detection using Dual Working Electrode and Concentration Process in Electrochemical Metalloimmunoassay

Cited by 6 publications

References 28 publications

Silver nanoparticles in electrochemical immunosensing and the emergence of silver–gold galvanic exchange detection

Silver nanoparticles in electrochemical immunosensing and the emergence of silver–gold galvanic exchange detection

Electrochemical Detection and Characterization of Nanoparticles with Printed Devices

Design of dual working electrodes for concentration process in metalloimmunoassay

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