Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil

Yu, Lili; Zhang, Yang; Hu, Chenyi; Wu, Hui; Yang, Yin; Huang, Chusen; Jia, Nengqin

doi:10.1016/j.foodchem.2014.12.030

Cited by 122 publications

(48 citation statements)

References 20 publications

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“…Alternative materials have been used for developing stable immunosensors in order to meet the growing demands of onsite environmental monitoring of trace pollutants, including the gold nanoparticles [27], carbon nanotubes [15,30], and AuNCs, which have attracted special attention due to the facile synthesis protocol, subnanometer size, and low toxicity [31]. Indeed, these AuNCs are excellent for quantitative studies of nanoparticle-protein interactions.…”

Section: Resultsmentioning

confidence: 99%

“…5a, b) and therefore were selected for BSA-AuNCmodified GCE immobilization. The incubation time and the pH of the solution directly affect the EIS analysis as antibody activity was highly time and pH dependent [15]. For optimization of the antigen incubation time and pH value of the reaction solution, the antibody-modified electrode was immersed in LMG solution (10 ng/mL) at the range of the pH (6.0~8.5) and incubation time (10~60 min).…”

Section: Optimization Of the Conditions During The Assembly Processmentioning

confidence: 99%

“…Among these, the electrochemical impedance-based immunosensor (EIS) [13,14] is one of the most efficient and sensitive methods. An impedimetric immunosensor is based on the detection of the binding between an immobilized antibody and an antigen [15] and is more amenable for miniaturization and economization [16]. Some electrochemical impedimetric immunosensors have been built to detect contaminants, but the detection of MG and LMG has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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Application of malachite green (MG) and leucomalachite green (LMG) in fish farm water causes an environmental problem. This study proposes for the first time a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining MG and LMG by a bovine serum albumin-decorated gold nanocluster (BSA-AuNC)/antibody composite film-based immunosensor. In order to improve the analytical performance, the glassy carbon electrode (GCE) was modified with 1, 4-phenylenediamine to form a stable layer, and then, BSA-AuNCs were covalently bound to the GCE. An adequate quantity of the polyclonal antibody of LMG was immobilized onto the surface of the BSA-AuNCs by the chemical reaction of EDC/NHS. The sensors can respond to the specific target based on specific covalent bonding. The experimental parameters, such as the pH, incubating concentration, and time, have been investigated and optimized. The calibration curve for LMG was linear in the range of 0.1~10.0 ng/mL with the limit of detection (LOD) 0.03 ng/mL. Furthermore, the sum of MG and LMG was detected in fish farm water by MG reduction. The recovery was between 89.7 % and 99.2 % in spiked samples. The EC sensor method was also compared with the ELISA method and validated by the LC-MS/MS method, which proves its great promise as a field instrument for the rapid monitoring of MG and LMG pollution. Graphical abstract 1, 4-Phenylenediamine and BSA-AuNC/antibody-decorated glassy carbon electrodes have been used for the impedimetric detection of the sum of malachite green and leucomalachite green via specific immuno-binding.

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Section: Resultsmentioning

confidence: 99%

Section: Optimization Of the Conditions During The Assembly Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A sensitive electrochemical impedance immunosensor for determination of malachite green and leucomalachite green in the aqueous environment

Zhu

Pang

et al. 2016

Anal Bioanal Chem

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“…Various electrochemical immunosensors using nanoparticles have been reported for these compounds [56]. Table 1 summarizes the analytical characteristics of recent immunosensors constructed for aflatoxin B1 (AFB1) [57][58][59][60]. A relevant example is the strategy involving a Chit-AuNPs nanocomposite electrodeposited onto the surface of a disk-ring gold microelectrode to prepare a label-free impedimetric immunosensor for AFB1 in wheat samples [57].…”

Section: Introductionmentioning

confidence: 99%

“…A composite prepared by mixing MWCNTs and the liquid ionic 1-butyl-3-methyl-imidazolium hexafluorophosphate ([BMIM]PF 6 ) was dropped onto a GCE and used as a medium to achieve fast electron transfer and a favorable microenvironment for antibody immobilization. The resulting anti-AFB1/IL/MWCNTs/GCE provided a LOD value of 0.03 ng·mL −1 and made possible the aflatoxin determination in real samples after extraction with methanol/water [60].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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