Highly sensitive glucose sensors based on enzyme-modified whole-graphene solution-gated transistors

Zhang, Meng; Liao, Caizhi; You, Peng; Mak, Chee Leung; Yan, Feng

doi:10.1038/srep08311

Cited by 187 publications

(112 citation statements)

References 39 publications

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“…However, glucose sensing is also important, which is explained below. 54 and 60 nM for Au. 55 It is expected that H 2 O 2 detection limit by using our sensor can be reduced further if the density of core-shell CdSe-ZnS nanoparticle is increased, which is one of the reasons.…”

Section: Resultsmentioning

confidence: 99%

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Kumar

Maikap

Singh

et al. 2016

J. Electrochem. Soc.

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A simple, label-free and cost effective sensor have been studied for reliable urea/glucose sensing, and common serum analyte detection comparable with market available urea "Assay Kit" is also performed by using SiO 2 and CdSe-ZnS nanoparticles in electrolyte-insulator-semiconductor structure for the first time. Thermally grown SiO 2 membrane has shown lower pH detection limit (0.081) and lowest drift rate (2.9 mV/hr) than those of the sputtering and E-beam deposited SiO 2 membranes. The urea detection at physiological buffer pH 7.4 with sensitivity of ∼1.6 mV/mg.dl −1 at linear range of 6 to 36 mg/dl is shown. The pH detection limit is further reduced (0.074) by using chaperonin protein mediated CdSe-ZnS nanoparticles assembly over SiO 2 surface owing to high pH sensitivity of 55 mV/pH. The sensing mechanism is due to the SiO x content decreased with increasing pH value. This suggests the lower H + ions absorption on the sensing membrane surface, which is observed by X-ray photo-electron spectroscopy. The glucose concentration is detected by using the core-shell CdSe-ZnS nanoparticles through H 2 O 2 sensing because of reduction/oxidation (redox) properties of Zn as well as Zn 2+ ions generation. Due to the high catalytic activity for H 2 O 2 sensitivity, low detection limit of 1 μM is obtained, which will help to detect glucose using this bio-chip in future. The surface charge as well as potential variation of sensing membranes corresponds to ionic concentration can be used for detection of bio-molecules. [3][4][5] Basically, the pH as well as ionic concentration change (H + and OH − ) is major product of enzymatic reaction with analytes (urea, creatinine, acetylcholine, tributyrine, glucose, etc. 6-8 ), which can be measured by using electrolyte-insulator-semiconductor (EIS) structures. Among those analytes, urea and glucose are the very common potential biomarkers for diagnosis of common diseases such as heart failure, diabetes, and kidney injury. [9][10][11][12] Generally, the urea concentration has been detected through pH change. 13,14 They have reported the detection of glucose concentration by measuring the resultant pH change in presence of glucose oxidase. However, small change of pH restricts the glucose measurement at lower concentration and encourages to sense other catalysis product. Hydrogen-peroxide (H 2 O 2 ) is also a catalysis product of selective enzymatic degradation of glucose, cholesterol, lactate, and so on. [15][16][17] In this regards, the sensing membrane in EIS structure has a key role to detect pH or H 2 O 2 for easy use in our daily life with low cost. Consequently, many materials such as SiO 2 , Si 3 N 4 , Al 2 O 3 , HfO 2 , and Ta 2 O 5 have been investigated for pH sensing.18-21 Hal et al. 22 have reported the high intrinsic buffer capacity of SiO 2 at low concentrated basic pH buffer results into higher surface sites and membrane-electrolyte interface reactivity. Due to very simple structure and good interface with substrate, SiO 2 is a reliable membrane to develop urea senso...

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Section: Resultsmentioning

confidence: 99%

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Kumar

Maikap

Singh

et al. 2016

J. Electrochem. Soc.

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“…are much lower (~0.01 %) than that in plasma. [196] Conventional electrochemical glucose sensors are not sensitive enough for these applications. On the other hand, detection of glucose with the aid of electronic devices such as GFETs can reach high sensitivity down to nM concentrations (see Tab.…”

Section: Gfet Glucose Dna and Protein Biosensorsmentioning

confidence: 99%

Sensing at the Surface of Graphene Field‐Effect Transistors

et al. 2016

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Abstract. 10Recent research trends now offer new opportunities for developing the next generations of label-free biochemical sensors using graphene and other two-dimensional materials. While the physics of graphene transistors operated in electrolyte is well grounded, important chemical challenges still remain to be addressed, namely the impact of the chemical functionalizations of graphene on the key electrical parameters and the sensing performances. In fact, graphene -at least ideal graphene -is highly chemically inert. The 15 functionalizations and chemical alterations of the graphene surface -both covalently and non-covalently -are crucial steps that define the sensitivity of graphene. The presence, reactivity, adsorption of gas and ions, proteins, DNA, cells and tissues on graphene have been successfully monitored with graphene. This review aims to unify most of the work done so far on biochemical sensing at the surface of a (chemically functionalized) graphene field-effect transistor and the challenges that lie ahead. We are convinced that graphene biochemical sensors 20 hold great promises to meet the ever-increasing demand for sensitivity, especially looking at the recent progresses suggesting that the obstacle of Debye screening can be overcome.2

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“…For example, solution-gated field effect transistors (SGFETs) based on carbon nanotubes for direct detection of Heroin metabolites were developed [1]; or graphene coatings were widely studied for pH [2], DNA or glucose sensing applications [3]. Last but not least carbon material, a diamond film opened new perspectives in the field of tissue engineering, prosthetics and microbiology.…”

Section: Introductionmentioning

confidence: 99%

Influence of Buffers and Culture Media on Diamond Solution-Gated Field Effect Transistors Regarding Stability and Memory Effect

Procházka

Ižák

Kromka

2017

Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017

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Abstract:The transfer characteristics of a nanocrystalline diamond (NCD)-based solution-gated field effect transistor (SGFET) under the influence of inorganic and organic compounds were studied. Studied compounds included three different buffer solutions (Phosphate, HEPES, McIlvaine buffer) and commonly used culture media (fibronectin, albumin and fetal bovine serum). It was found that buffers with the same pH of 7.4 caused different voltage shifts in transfer characteristics. This effect was reversible which indicates the surface stability of the hydrogen-terminated diamond during repeated measurements. In contrast to this observation, the SGFET sensitivity decreased after applying the culture solutions which we attribute to the permanently adsorbed bio-layer formed on the SGFET channel sensing area.

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Highly sensitive glucose sensors based on enzyme-modified whole-graphene solution-gated transistors

Cited by 187 publications

References 39 publications

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Sensing at the Surface of Graphene Field‐Effect Transistors

Influence of Buffers and Culture Media on Diamond Solution-Gated Field Effect Transistors Regarding Stability and Memory Effect

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Highly sensitive glucose sensors based on enzyme-modified whole-graphene solution-gated transistors

Cited by 187 publications

References 39 publications

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO2and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO2and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Sensing at the Surface of Graphene Field‐Effect Transistors

Influence of Buffers and Culture Media on Diamond Solution-Gated Field Effect Transistors Regarding Stability and Memory Effect

Contact Info

Product

Resources

About

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure

Highly Reliable Label-Free Detection of Urea/Glucose and Sensing Mechanism Using SiO₂and CdSe-ZnS Nanoparticles in Electrolyte-Insulator-Semiconductor Structure