Highly sensitive HPLC-MS/MS assay for the quantitation of ondansetron in rat plasma and rat brain tissue homogenate following administration of a very low subcutaneous dose

Gaudette, Fleur; Bédard, Dominique; Kwan, Cynthia; Frouni, Imane; Hamadjida, Adjia; Beaudry, Francis

doi:10.1016/j.jpba.2019.07.014

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…The following combinations of experimental conditions were tested: different volumes of n-hexane (1, 2, 3, and 5 mL), different times of shaking (5, 10, 15, or 20 min) at 250 rpm, and different times of centrifuging (15,20,25, or 30 min) at 5800 rpm.…”

Section: Development Of Sample Preparation Proceduresmentioning

confidence: 99%

“…Several bioanalytical methods have been reported in the literature for the quantification of OND, for example, spectrophotometric [20] and more popular liquid chromatography with UV [21][22][23][24] or MS [25][26][27][28][29] detection. Raza et al [20] estimated drug concentration in bulk material using the spectrophotometry method.…”

Section: Introductionmentioning

confidence: 99%

“…Raza et al [20] estimated drug concentration in bulk material using the spectrophotometry method. Numerous papers described OND determination in biological matrixes [26,29], i.e., human plasma or animal tissues in rats [22,25], cats [27], and dogs [30]. Investigations on the determination of OND, together with other drugs in infusion fluids, constitute a separate part.…”

Section: Introductionmentioning

confidence: 99%

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Application of the HPLC Method in Parenteral Nutrition Assessment: Stability Studies of Ondansetron

et al. 2021

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Ondansetron (OND) is a serotonin type 3 receptor antagonist that exhibits antiemetic activity. From the clinical point of view, vomiting and nausea prevention is an important task. Anticancer treatment and recovery impact the patient’s overall state by affecting appetite, well-being, and physical activity, and consequently, nutrition quality. Depending on the patient’s indication and condition, parenteral nutrition is administered to meet full nutritional requirements. In addition, antiemetic drugs can be added to the parenteral nutrition (PN) admixture to treat chemo- or radio-therapy-induced nausea and vomiting. However, adding any medication to the PN admixture can result in the instability of the composition in the overall admixture. This study aimed to develop the HPLC method of determination of OND in Lipoflex special, one of the most popular, ready-to-use PN admixtures. The proposed HPLC method and the sample preparation procedure were suitable for analyzing OND in PN admixture stored under various conditions, such as exposure to sunlight and temperature. It was found that the decomposition of OND during the seven-day storage did not exceed 5% and did not depend on external factors. Based on the conducted research, it is recommended to add OND to Lipoflex special, and it is possible to store such an admixture for seven days.

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Section: Development Of Sample Preparation Proceduresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Application of the HPLC Method in Parenteral Nutrition Assessment: Stability Studies of Ondansetron

et al. 2021

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“…Analyzing NTs and their metabolites in biological samples are challenging because they are found in low quantities and have a high tendency to oxidize [ 19 , 20 ]. The use of many analytical techniques to determine NTs in biological samples has been developed.…”

Section: Introductionmentioning

confidence: 99%

Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects of Althaea rosea Flower on TTX-Intoxicated Rats

Wang

Zheng

et al. 2023

Molecules

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Tetrodotoxin (TTX) inhibits neurotransmission in animals, and there is no specific antidote. In clinical practice in China, Althaea rosea (A. rosea flower) extract has been used to treat TTX poisoning. In this work, the efficacy of the ethyl acetate fraction extract of A. rosea flower in treating TTX poisoning in rats was investigated. A high-performance liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed to determine nine neurotransmitters in rat brain tissue, including γ-aminobutyric acid (GABA), dopamine (DA), 5-hydroxytryptamine (5-HT), noradrenaline (NE), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindole-3-acetic acid (5-HIAA), epinephrine (E), and tyramine (Tyn). The detoxifying effect of A. rosea flower was verified by comparing the changes in neurotransmitters’ content in brain tissue before and after poisoning in rats. The assay was performed in multiple reaction monitoring mode. The quantification method was performed by plotting an internal-standard working curve with good linearity (R2 > 0.9941) and sensitivity. Analyte recoveries were 94.04–107.53% (RSD < 4.21%). Results indicated that the levels of 5-HT, DA, E, and NE in the brains of TTX-intoxicated rats decreased, whereas the levels of GABA, Tyn, and 5-HIAA showed an opposite trend, and HVA and DOPAC were not detected. The levels of all seven neurotransmitters returned to normal after the gavage administration of ethyl acetate extract of A. rosea flower to prove that the ethyl acetate extract of A. rosea flower had a therapeutic effect on TTX poisoning. The work provided new ideas for studies on TTX detoxification.

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“…Epilepsy is the second most common disease in the nervous system resulting from recurrent and complicated central nervous system (CNS) dysfunction, due to abnormal brain neurons [1, 2]. Due to its repeated, irregular, and sudden onset, patients are prone to physical injury and mental stress, which poses a serious threat to global health [3, 4]. The incidence of epilepsy in China appears to be increasing each year [5, 6].…”

mentioning

confidence: 99%

Cu‐Zn SOD suppresses epilepsy in pilocarpine‐treated rats and alters SCN2A/Nrf2/HO‐1 expression

Wen

Tan

Xiang

2022

Epileptic Disorders

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Objective. Copper-zinc superoxide dismutase (Cu-Zn SOD) is downregulated in epilepsy, however, the role of Cu-Zn SOD in epilepsy remains unclear. Methods. Based on the pilocarpine hydrochloride-induced rat model of epilepsy, cortical-striatum brain slices of rats were examined based on field excitatory post-synaptic potentials. Pathological changes were observed by transmission electron microscope. Also using SH-SY5Y cells, flow cytometry and TUNEL staining were applied to investigate cell apoptosis, and ELISA was applied to detect SOD activity. In addition, qRT-PCR and western blot were performed to detect SCN2A/Nrf2/HO-1 gene and protein expression levels, respectively. Results. Cu-Zn SOD over-expression suppressed epilepsy in vivo. In addition, Cu-Zn SOD knockdown notably decreased SOD activity and induced apoptosis in SH-SY5Y cells. Moreover, Cu-Zn SOD silencing decreased the levels of SCN2A, Nrf2 and HO-1. Lastly, Cu-Zn SOD was shown to modulate the NaV1.2/ Nrf2/HO-1 axis in rats.Significance. In this model, Cu-Zn SOD attenuated epilepsy and was shown to alter the expression level of proteins of the NaV1.2 /Nrf2/HO-1 signalling pathway, indicating that Cu-Zn SOD might be a target for the treatment of epilepsy.

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Highly sensitive HPLC-MS/MS assay for the quantitation of ondansetron in rat plasma and rat brain tissue homogenate following administration of a very low subcutaneous dose

Cited by 9 publications

References 31 publications

Application of the HPLC Method in Parenteral Nutrition Assessment: Stability Studies of Ondansetron

Application of the HPLC Method in Parenteral Nutrition Assessment: Stability Studies of Ondansetron

Determination of Multiple Neurotransmitters through LC-MS/MS to Confirm the Therapeutic Effects of Althaea rosea Flower on TTX-Intoxicated Rats

Cu‐Zn SOD suppresses epilepsy in pilocarpine‐treated rats and alters SCN2A/Nrf2/HO‐1 expression

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