Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food

Bartosh, Anastasiya V.; Urusov, Alexandr Е.; Petrakova, Alina V.; Kuang, Hua; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.1080/09540105.2020.1750570

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…Mycotoxin analysis is a topical issue and many researchers have reported various LFAs with different labels for the sensitive detection of mycotoxins. [ 145 ] Because these toxins are often present together in agricultural crops, they tend to be monitored simultaneously. [ 16,27a,63b,146 ] For example, Xiong's group [ 147 ] developed a dual‐signal‐based LFA for the multiplex detection of mycotoxins, which used a smartphone‐based device to integrate both visible‐light and fluorescence detection modes.…”

Section: Applicationsmentioning

confidence: 99%

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang

Zhao

et al. 2021

Small Methods

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The development of the lateral flow assay (LFA) has received much attention in both academia and industry because of their broad applications to food safety, environmental monitoring, clinical diagnosis, and so forth. The user friendliness, low cost, and easy operation are the most attractive advantages of the LFA. In recent years, quantitative detection has become another focus of LFA development. Here, the most recent studies of quantitative LFAs are reviewed. First, the principles and corresponding formats of quantitative LFAs are introduced. In the biomaterial and nanomaterial sections, the detection, capture, and signal amplification biomolecules and the optical, fluorescent, luminescent, and magnetic labels used in LFAs are described. The invention of dedicated strip readers has drawn further interest in exploiting the better performance of LFAs. Therefore, next, the development of dedicated reader devices is described and the usefulness and specifications of these devices for LFAs are discussed. Finally, the applications of LFAs in the detection of metal ions, biotoxins, pathogenic microorganisms, veterinary drugs, and pesticides in the fields of food safety and environmental health and the detection of nucleic acids, biomarkers, and viruses in clinical analyses are summarized.

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Section: Applicationsmentioning

confidence: 99%

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang

Zhao

et al. 2021

Small Methods

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“…ICAs not only have high sensitivity and specificity but also do not require complicated instruments and can produce results in a few minutes, features that are important for rapid screening and real-time detection. In the study of Dzantiev et al [ 152 ], a scheme for competitive ICA with indirect labeling was implemented and developed for the detection of ZEN in baby food. Two separate reagents, free specific Abs and anti-species Abs conjugated with AuNPs (30 nm), were used to improve the sensitivity and the reliability of measurements at the same time.…”

Section: Nanoscale Materials For Rapid Detection and Screeningmentioning

confidence: 99%

Nanoscale Materials Applying for the Detection of Mycotoxins in Foods

Hu,

Li,

Yang

et al. 2023

Foods

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Trace amounts of mycotoxins in food matrices have caused a very serious problem of food safety and have attracted widespread attention. Developing accurate, sensitive, rapid mycotoxin detection and control strategies adapted to the complex matrices of food is crucial for in safeguarding public health. With the continuous development of nanotechnology and materials science, various nanoscale materials have been developed for the purification of complex food matrices or for providing response signals to achieve the accurate and rapid detection of various mycotoxins in food products. This article reviews and summarizes recent research (from 2018 to 2023) on new strategies and methods for the accurate or rapid detection of mold toxins in food samples using nanoscale materials. It places particular emphasis on outlining the characteristics of various nanoscale or nanostructural materials and their roles in the process of detecting mycotoxins. The aim of this paper is to promote the in-depth research and application of various nanoscale or structured materials and to provide guidance and reference for the development of strategies for the detection and control of mycotoxin contamination in complex matrices of food.

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“…The corn flour was pretreated according to the protocol [55,56]. Briefly, corn flour was extracted with a mixture of methanol and water in a ratio of 30:70.…”

Section: Sample Preparationmentioning

confidence: 99%

A Fluorescence Resonance Energy Transfer Aptasensor for Aflatoxin B1 Based on Ligand-Induced ssDNA Displacement

Serebrennikova,

Samokhvalov,

Zherdev

et al. 2023

Molecules

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In this study, a fluorescence resonance energy transfer (FRET)-based aptasensor for the detection of aflatoxin B1 (AFB1) was designed using a carboxyfluorescein (FAM)-labeled aptamer and short complementary DNA (cDNA) labeled with low molecular quencher RTQ1. The sensing principle was based on the detection of restored FAM-aptamer fluorescence due to the ligand-induced displacement of cDNA in the presence of AFB1, leading to the destruction of the aptamer/cDNA duplex and preventing the convergence of FAM and RTQ1 at the effective FRET distance. Under optimal sensing conditions, a linear correlation was obtained between the fluorescence intensity of the FAM-aptamer and the AFB1 concentration in the range of 2.5–208.3 ng/mL with the detection limit of the assay equal to 0.2 ng/mL. The assay time was 30 min. The proposed FRET aptasensor has been successfully validated by analyzing white wine and corn flour samples, with recovery ranging from 76.7% to 91.9% and 84.0% to 86.5%, respectively. This work demonstrates the possibilities of labeled cDNA as an effective and easily accessible tool for sensitive AFB1 detection. The homogeneous FRET aptasensor is an appropriate choice for contaminant screening in complex matrices.

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Highly sensitive lateral flow test with indirect labelling for zearalenone in baby food

Cited by 12 publications

References 44 publications

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Nanoscale Materials Applying for the Detection of Mycotoxins in Foods

A Fluorescence Resonance Energy Transfer Aptasensor for Aflatoxin B1 Based on Ligand-Induced ssDNA Displacement

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