Highly sensitive LC–MS/MS methods for urinary biological monitoring of occupational exposure to cyclophosphamide, ifosfamide, and methotrexate antineoplastic drugs and routine application

Canal-Raffin, Mireille; Khennoufa, Karim; Martínez, Béatrice; Goujon, Yves; Folch, Cinta; Ducint, Dominique; Titier, Karine; Brochard, Patrick; Verdun-Esquer, Catherine; Molimard, Mathiéu

doi:10.1016/j.jchromb.2016.10.021

Cited by 30 publications

(37 citation statements)

References 27 publications

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“…A second extraction technique liquid-liquid extraction (LLE) was used in several nitrogen mustard methods [21,22, 25, 28, 29,30] and in the methods for taxanes (e.g. docetaxel) [23, 32].…”

Section: Tabular Summaries Of Selected Methodsmentioning

confidence: 99%

“…In four of the methods for their quantitation (Table 1), CP is the single target using either IF [21,24] or deuterated-CP [22,23] as an internal standard (IS). The remainder target both CP and IF using structural analogs phencyclidine, trophosphamide, and prednisolone as ISs [25–27], while three target CP, IF and one or more stable metabolites using a deuterated-CP IS [28–30]. The majority use sample preparation with either LLE or SPE C18 with ethylacetate to remove matrix interferences, while one method uses methanol to remove protein while capturing polar CP metabolites [30].…”

Section: Overview Of the Methodsmentioning

confidence: 99%

“…The methods summarized in Table 3 have similar chromatographic and detection conditions using acidic mobile phases to promote positive ion formation for MRM + [28, 36] or SRM + detection [37, 38] and all use similar mass transitions for detection and quantification. Rule et al [37], uses a microbore column (2mm) and focuses on a high-throughput determination of MTX and its metabolite 7-OH-MTX, The method features a multi-well plate SPE C18 extraction, 0.15 ml/minute flow rate and a 2.2 minute run-time [37].…”

Section: Overview Of the Methodsmentioning

confidence: 99%

“…Oddly, neither method includes the amount of urine that was extracted to achieve the reported LOD values. In contrast, Turci et al [36] and Canal-Raffin et al [28] developed determinations specifically for biomonitoring of healthcare workers. Turci et al, [36] used manual SPE and a conventional RP analytical column.…”

Section: Overview Of the Methodsmentioning

confidence: 99%

“…A 10 μl injection provided for an LOD of 0.2 μg/L for MTX as single the analyte [36]. In the more recent method, Canal-Raffin et al, [28] also used SPE with HAX medium that combines non-polar (C8) interactions and strong anion exchange for analyte concentration. To remove matrix components from 5 ml of urine, samples were diluted with an equal volume of formate buffer.…”

Section: Overview Of the Methodsmentioning

confidence: 99%

See 4 more Smart Citations

A review of high performance liquid chromatographic-mass spectrometric urinary methods for anticancer drug exposure of health care workers

Mathias

Connor

B’Hymer

2017

Journal of Chromatography B

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The medical treatment of cancer with antineoplastic drugs is routine, but careful biomonitoring for these powerful drugs in individual healthcare worker exposure is necessary to ensure that engineering controls and exposure intervention measures are effective. This review describes published high performance liquid chromatography/mass spectrometry (HPLC-MS) methods for the determination of anticancer drugs in human urine as non-invasive tools for monitoring of healthcare worker exposure to antineoplastic and cytotoxic drugs. HPLC-MS is a sensitive and specific method for analysis of anticancer drugs and their metabolites in biological fluids. In this review, a tabular summary and overview of published HPLC-MS methods are presented, as well as future trends and limitations in this area of research.

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“…A second extraction technique liquid-liquid extraction (LLE) was used in several nitrogen mustard methods [21,22, 25, 28, 29,30] and in the methods for taxanes (e.g. docetaxel) [23, 32].…”

Section: Tabular Summaries Of Selected Methodsmentioning

confidence: 99%

Section: Overview Of the Methodsmentioning

confidence: 99%

Section: Overview Of the Methodsmentioning

confidence: 99%

Section: Overview Of the Methodsmentioning

confidence: 99%

Section: Overview Of the Methodsmentioning

confidence: 99%

See 3 more Smart Citations

A review of high performance liquid chromatographic-mass spectrometric urinary methods for anticancer drug exposure of health care workers

Mathias

Connor

B’Hymer

2017

Journal of Chromatography B

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Bioinspired Polyoxomolybdate Nanoclusters Peroxidase‐Mimicking Nanozyme for Dual‐Mode Detection of Methotrexate in Real Samples

Azarian,

Dehghan,

Amini

2024

Applied Organom Chemis

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Methotrexate (MTX) is a chemotherapy agent and immune system suppressant that is widely used to treat a variety of malignant and nonmalignant diseases. Due to some drawbacks of the existing methods for the determination of MTX in aquatic and real samples, we prepared a new, simple, and selective dual‐mode fluorometric and electrochemical approach based on peroxidase‐mimic [Mo36] polyoxomolybdate nanoclusters (POMo) for sensing MTX. A turn‐on–off fluorometric approach for the detection of MTX was developed, which was based on the inhibition of the emission intensity of the terephthalic acid (TA)‐H2O2 system upon the addition of MTX to the reaction solution. The electrocatalytic performance of the obtained POMo was studied after making a carbon paste electrode (CPE) utilizing cyclic voltammetry and amperometry analysis. Under the optimum condition, good linearity was observed between the quenched emission intensity of the system and MTX dosage in the range of 0.5–250 μM with a limit of detection (LOD) of 0.91 μM. The electrochemical method illustrated a much better response with an extensive linear range of 0.01–475 μM and a lower LOD (1.61 nM). Analytical recoveries and RSD% values were, respectively, in the range of 87.72%–110% and 0.33%–1.32% for the fluorometric system and 96%–106% and 0.77%–1.02% for the electrochemical platform. Finally, the selectivity of the sensor was evaluated against various common interfering species, and the results indicated satisfactory selectivity of the POMo dual‐mode system towards MTX.

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A review of bioanalytical methods for chronic lymphocytic leukemia drugs and metabolites in biological matrices

Suresh

Trivedi

Srinivas³

et al. 2019

Biomedical Chromatography

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Quantitation of drugs used for the treatment of chronic lymphocytic leukemia in various biological matrices during both pre‐clinical and clinical developments is very important, often in routine therapeutic drug monitoring. The first developed methods for quantitation were traditionally done on LC in combination with either UV or fluorescence detection. However, the emergence of LC with mass spectrometry in tandem in early 1990s has revolutionized the quantitation as it has provided better sensitivity and selectivity within a shorter run time; therefore it has become the choice of method for the analysis of various drugs. In this article, an overview of various bioanalytical methods (HPLC or LC–MS/MS) for the quantification of drugs for the treatment of chronic lymphocytic leukemia, along with applicability of these methods, is given.

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Highly sensitive LC–MS/MS methods for urinary biological monitoring of occupational exposure to cyclophosphamide, ifosfamide, and methotrexate antineoplastic drugs and routine application

Cited by 30 publications

References 27 publications

A review of high performance liquid chromatographic-mass spectrometric urinary methods for anticancer drug exposure of health care workers

A review of high performance liquid chromatographic-mass spectrometric urinary methods for anticancer drug exposure of health care workers

Bioinspired Polyoxomolybdate Nanoclusters Peroxidase‐Mimicking Nanozyme for Dual‐Mode Detection of Methotrexate in Real Samples

A review of bioanalytical methods for chronic lymphocytic leukemia drugs and metabolites in biological matrices

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