2008
DOI: 10.1016/j.jchromb.2008.03.005
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Highly sensitive method for quantitative determination of bilirubin in biological fluids and tissues

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Cited by 75 publications
(57 citation statements)
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“…Tissue Bilirubin Quantification UCB content was determined on rapidly thawed samples using high-performance liquid chromatography with diode array detector (Agilent, Santa Clara, CA) as described (10). Briefly, 300 pmol of mesobilirubin in dimethylsulfoxide (internal standard) was added, and samples were homogenized on ice.…”
Section: Animal Sacrifice and Sample Collectionmentioning
confidence: 99%
See 1 more Smart Citation
“…Tissue Bilirubin Quantification UCB content was determined on rapidly thawed samples using high-performance liquid chromatography with diode array detector (Agilent, Santa Clara, CA) as described (10). Briefly, 300 pmol of mesobilirubin in dimethylsulfoxide (internal standard) was added, and samples were homogenized on ice.…”
Section: Animal Sacrifice and Sample Collectionmentioning
confidence: 99%
“…The first aim of this work was to apply a sensitive, specific high-performance liquid chromatography method (10) to assess tissue UCB levels spontaneously present in Gunn (jj) rats with jaundice due to a homozygous mutation, leading to loss of function of bilirubin-UDP glucuronosyl transferase (UGT1A1), and their heterozygous (Jj) and wild-type (JJ) littermates. The second aim was to study serial changes in regional brain UCB levels when jj Gunn rats were treated with sulphadimethoxine to acutely displace UCB from albumin, mimicking the effects of acute increases in unconjugated hyperbilirubinemia that can occur in jaundiced newborns and patients with Crigler-Najjar type I.…”
mentioning
confidence: 99%
“…Serum bilirubin levels were measured using a BNISTAT Bilirubinometer (Reichert Technologies, USA). Tissue extraction and bilirubin quantification for bilirubin measurement in the brain tissue were modified based on the assay published previously (18). In brief, the brain tissue (100 mg) was disintegrated to a fine suspension by grinding and subsequently diluted with extraction solvent: methanol/chloroform/n-hexane (63:31:6, v/v).…”
Section: Methodsmentioning
confidence: 99%
“…Concentrations of biliary UCB and bilirubin-glucuronic acid conjugates in 2-month-old mice were determined by HPLC as previously described by Zelenka et al (2008) and by Spivak and Carey (1985), respectively.…”
Section: Bilirubin Measurementsmentioning
confidence: 99%
“…Quantitative determination of UCB in tissues of 2-monthold mice was performed as previously described by Zelenka et al (2008).…”
Section: Bilirubin Measurementsmentioning
confidence: 99%