Highly Sensitive Molecularly Imprinted Electrochemical Sensor Based on the Double Amplification by an Inorganic Prussian Blue Catalytic Polymer and the Enzymatic Effect of Glucose Oxidase

Abstract

A novel strategy to improve the sensitivity of molecularly imprinted polymer (MIP) sensors was proposed. An electrocatalytic Prussian blue (PB) film was electrochemically polymerized on an electrode surface to fabricate an MIP electrochemical sensor using oxytetracycline (OTC) as a template. The OTC determination relied on a competition reaction between OTC and glucose-oxidase-labeled OTC and the catalytic reduction of hydrogen peroxide by the modified PB film. Experimental results show that double amplification, which is based on the catalysis of inorganic PB films and the enzymatic effect of glucose oxidase, can remarkably increase the assay sensitivity. The main experimental conditions (including electrocatalysis of the PB film, pH effects, incubation and competition times, and anti-interference) were optimized. This novel MIP sensor can offer an femtomole detection limit for OTC. In addition, the feasibility of its practical applications has been demonstrated in the analysis of a series of real milk samples.

A novel strategy to improve the sensitivity of antibiotics determination based on bioelectrocatalysis at molecularly imprinted polymer film electrodes

“…The procedure and strategy for the CV measurement of KA by the MIP film system coupled with bioelectrocatalysis is illustrated in Scheme 1. While the enzymatic reaction was introduced in the voltammetric determination of some analytes at MIP film electrodes by Li's group to improve the detection sensitivity, the "isolation-incubationcompetition" procedure was quite complicated and time-consuming (Li et al, 2010(Li et al, , 2012b. Thus, our novel strategy for the direct amplification of CV response of the electroactive probe by bioelectrocatalysis in the testing solution at MIP film electrodes shows obvious advantages.…”

Multisensor System Based on Layer by Layer Films. Discrimination of Milks

“…Moreover, hybrid structures can be developed combining different materials in the layered structure that can improve the final sensing properties. Finally, it is an advantageous method to immobilize enzymes in a biomimetic environment [11][12][13][14][15] 2 of 13 Phthalocyanines have been widely used as sensing materials in electrochemical sensors due to their chemical reactivity, their excellent electrocatalytic properties as well as the capability to form LbL nanostructured films [11,16]. The LbL technique can be used to combine the water soluble anionic metal tetrasulfonated phthalocyanines (MPc S ) with other cationic materials to obtain hybrid layers with improved catalytic properties [17].…”

Facile and controllable one-step fabrication of molecularly imprinted polymer membrane by magnetic field directed self-assembly for electrochemical sensing of glutathione

“…Among these methods, electrochemical method has attracted tremendous attention because of its higher sensitivity, lower cost and faster response compared with other methods [18][19][20]. Meanwhile, GSH is an electroactive compound which can be oxidized to glutathione disulphide (GSSG) by the loss of 2 M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 4 protons and 2 electrons [21,22].…”